本文主要研究内容
作者范现成（2019）在《毒害艾美耳球虫感染雏鸡小肠lncRNAs、miRNAs和CircRNAs表达谱分析》一文中研究指出：毒害艾美耳球虫(Eimeria necatrix)是鸡球虫病中主要致病虫种之一,常寄生于鸡小肠和盲肠的上皮细胞内,导致受损肠道严重出血,病鸡常表现腹泻、血痢、生长缓慢等临床症状,有较高发病率和死亡率,对鸡的养殖造成严重威胁。目前,该病的防治主要依赖药物和疫苗,药物虽然具有较好的驱虫效果,但易产生药物残留和耐药性;疫苗在鸡球虫的预防控制中可有效避免化学药物带来的许多副作用,但其存在高成本、易散毒、免疫保护力不够等缺点。近些年来的研究表明,非编码RNA(non-coding RNA,ncRNA)可在宿主与寄生虫的相互作用中发挥重要的调节作用,特别是长链非编码RNA(long non-coding RNA,lncRNA)、环状RNA(Circular RNA,CircRNA)和微小RNA(microRNA,miRNA)参与了许多疾病的发生和发展过程,并已作为多种疾病防治的潜在靶标和诊断、预后的生物标志。基于此,本研究以E.necatrix感染雏鸡为模型,利用高通量测序技术系统研究与E.necatrix感染相关的宿主小肠的全转录组表达谱。1.获得了E.necatrix感染雏鸡小肠的mRNA和lncRNA表达谱:将8000个E.necatrix卵囊经口感染10 d的雏鸡,在感染后108 h取雏鸡小肠中段进行RNA-seq分析,共获得1543个显著差异表达的mRNA(707个上调mRNA和836个下调mRNA)和95个显著差异表达的lncRNA(49个上调lncRNA和46个下调lncRNA)。共表达网络分析显示,这些lncRNA和mRNA之间存在37134条共表达关系,其中38个lncRNA顺式调节73个mRNA,而87个lncRNA反式调节1453个mRNA。GO功能和KEGG通路富集分析发现,差异表达的lncRNA的靶基因显著富集于E.necatrix感染相关的AMPK、PPAR和JAK-STAT等免疫通路。2.获得了E.necatrix感染雏鸡小肠的miRNA表达谱:在雏鸡小肠中段组织中鉴定出1266个miRNA,包括663个已知的miRNA和603个新的miRNA。进一步研究发现,E.necatrix感染引起了雏鸡小肠35个miRNA显著差异表达(16个上调和19个下调);功能分析发现其中的21个miRNA的4568个靶mRNA显著富集于2047个GO条目(转录、转录后调控和粘附分子连接等)和54个KEGG通路(钙信号通路、MAPK信号通路和胰岛素信号通路等)。3.获得了E.necatrix感染雏鸡小肠的CircRNA表达谱:在雏鸡小肠中段组织中共鉴定出4153个CircRNA序列,E.necatrix感染引起了雏鸡小肠13个CircRNA显著差异表达(9个上调CircRNA和4个下调CircRNA)。功能分析发现,这些CircRNAs来源基因主要参与正调控NF-κB信号通路、磷脂酰肌醇信号通路等。CircRNA-miRNAmRNA互做网络分析发现,本研究中差异表达的CircRNAs可能通过吸附2个差异表达的miRNA,间接调控62个差异表达的靶mRNA,而这些靶mRNAs主要富集在MAPK、JAK-STAT等通路。综上所述,本研究运用RNA-seq技术对E.necatrix感染雏鸡的小肠中段组织进行测序分析发现,E.necatrix感染改变了小肠中的mRNAs、lncRNAs、miRNAs和CircRNAs表达谱,功能分析发现这些RNA分子可能参与了E.necatrix与雏鸡小肠的互作过程。这些研究结果为深入揭示E.necatrix与雏鸡的互作机制提供了基础数据。
Abstract
du hai ai mei er qiu chong (Eimeria necatrix)shi ji qiu chong bing zhong zhu yao zhi bing chong chong zhi yi ,chang ji sheng yu ji xiao chang he mang chang de shang pi xi bao nei ,dao zhi shou sun chang dao yan chong chu xie ,bing ji chang biao xian fu xie 、xie li 、sheng chang huan man deng lin chuang zheng zhuang ,you jiao gao fa bing lv he si wang lv ,dui ji de yang shi zao cheng yan chong wei xie 。mu qian ,gai bing de fang zhi zhu yao yi lai yao wu he yi miao ,yao wu sui ran ju you jiao hao de qu chong xiao guo ,dan yi chan sheng yao wu can liu he nai yao xing ;yi miao zai ji qiu chong de yu fang kong zhi zhong ke you xiao bi mian hua xue yao wu dai lai de hu duo fu zuo yong ,dan ji cun zai gao cheng ben 、yi san du 、mian yi bao hu li bu gou deng que dian 。jin xie nian lai de yan jiu biao ming ,fei bian ma RNA(non-coding RNA,ncRNA)ke zai su zhu yu ji sheng chong de xiang hu zuo yong zhong fa hui chong yao de diao jie zuo yong ,te bie shi chang lian fei bian ma RNA(long non-coding RNA,lncRNA)、huan zhuang RNA(Circular RNA,CircRNA)he wei xiao RNA(microRNA,miRNA)can yu le hu duo ji bing de fa sheng he fa zhan guo cheng ,bing yi zuo wei duo chong ji bing fang zhi de qian zai ba biao he zhen duan 、yu hou de sheng wu biao zhi 。ji yu ci ,ben yan jiu yi E.necatrixgan ran chu ji wei mo xing ,li yong gao tong liang ce xu ji shu ji tong yan jiu yu E.necatrixgan ran xiang guan de su zhu xiao chang de quan zhuai lu zu biao da pu 。1.huo de le E.necatrixgan ran chu ji xiao chang de mRNAhe lncRNAbiao da pu :jiang 8000ge E.necatrixluan nang jing kou gan ran 10 dde chu ji ,zai gan ran hou 108 hqu chu ji xiao chang zhong duan jin hang RNA-seqfen xi ,gong huo de 1543ge xian zhe cha yi biao da de mRNA(707ge shang diao mRNAhe 836ge xia diao mRNA)he 95ge xian zhe cha yi biao da de lncRNA(49ge shang diao lncRNAhe 46ge xia diao lncRNA)。gong biao da wang lao fen xi xian shi ,zhe xie lncRNAhe mRNAzhi jian cun zai 37134tiao gong biao da guan ji ,ji zhong 38ge lncRNAshun shi diao jie 73ge mRNA,er 87ge lncRNAfan shi diao jie 1453ge mRNA。GOgong neng he KEGGtong lu fu ji fen xi fa xian ,cha yi biao da de lncRNAde ba ji yin xian zhe fu ji yu E.necatrixgan ran xiang guan de AMPK、PPARhe JAK-STATdeng mian yi tong lu 。2.huo de le E.necatrixgan ran chu ji xiao chang de miRNAbiao da pu :zai chu ji xiao chang zhong duan zu zhi zhong jian ding chu 1266ge miRNA,bao gua 663ge yi zhi de miRNAhe 603ge xin de miRNA。jin yi bu yan jiu fa xian ,E.necatrixgan ran yin qi le chu ji xiao chang 35ge miRNAxian zhe cha yi biao da (16ge shang diao he 19ge xia diao );gong neng fen xi fa xian ji zhong de 21ge miRNAde 4568ge ba mRNAxian zhe fu ji yu 2047ge GOtiao mu (zhuai lu 、zhuai lu hou diao kong he nian fu fen zi lian jie deng )he 54ge KEGGtong lu (gai xin hao tong lu 、MAPKxin hao tong lu he yi dao su xin hao tong lu deng )。3.huo de le E.necatrixgan ran chu ji xiao chang de CircRNAbiao da pu :zai chu ji xiao chang zhong duan zu zhi zhong gong jian ding chu 4153ge CircRNAxu lie ,E.necatrixgan ran yin qi le chu ji xiao chang 13ge CircRNAxian zhe cha yi biao da (9ge shang diao CircRNAhe 4ge xia diao CircRNA)。gong neng fen xi fa xian ,zhe xie CircRNAslai yuan ji yin zhu yao can yu zheng diao kong NF-κBxin hao tong lu 、lin zhi xian ji chun xin hao tong lu deng 。CircRNA-miRNAmRNAhu zuo wang lao fen xi fa xian ,ben yan jiu zhong cha yi biao da de CircRNAske neng tong guo xi fu 2ge cha yi biao da de miRNA,jian jie diao kong 62ge cha yi biao da de ba mRNA,er zhe xie ba mRNAszhu yao fu ji zai MAPK、JAK-STATdeng tong lu 。zeng shang suo shu ,ben yan jiu yun yong RNA-seqji shu dui E.necatrixgan ran chu ji de xiao chang zhong duan zu zhi jin hang ce xu fen xi fa xian ,E.necatrixgan ran gai bian le xiao chang zhong de mRNAs、lncRNAs、miRNAshe CircRNAsbiao da pu ,gong neng fen xi fa xian zhe xie RNAfen zi ke neng can yu le E.necatrixyu chu ji xiao chang de hu zuo guo cheng 。zhe xie yan jiu jie guo wei shen ru jie shi E.necatrixyu chu ji de hu zuo ji zhi di gong le ji chu shu ju 。
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论文作者分别是来自西北农林科技大学的范现成，发表于刊物西北农林科技大学2019-07-11论文，是一篇关于毒害艾美耳球虫论文,表达谱论文,西北农林科技大学2019-07-11论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自西北农林科技大学2019-07-11论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。