本文主要研究内容
作者杨宗霖,王艺,马田田,霍春月,刘晓,阚云超,李丹丹（2019）在《有翅和无翅豌豆蚜中翅型分化信号通路相关微小RNA及其靶基因的表达差异》一文中研究指出：【目的】前期研究发现麦长管蚜Sitobion avenae孤雌蚜有翅和无翅个体中存在很多差异表达的微小RNA(microRNA, miRNA),本研究旨在进一步明确这些miRNA在豌豆蚜Acyrthosiphon pisum中发挥作用的发育阶段,探索miRNA调控孤雌蚜翅两型性分化的机制。【方法】选择在麦长管蚜有翅蚜和无翅蚜中显著差异表达,且靶基因为蜕皮激素、胰岛素信号通路及翅型发育关键基因的5个miRNA(Let-7,miR-92a,miR-92b,miR-92a-1-p5和miR-277),利用qPCR检测这些miRNA及其靶标基因在豌豆蚜3-4龄若蚜和成虫有翅和无翅个体中的表达谱;同时利用双荧光素酶活性检测法对上述miRNA的靶基因进行验证。【结果】表达谱分析发现,这5个miRNA在豌豆蚜成虫中表达量均高于其在若蚜中的表达量,而其预测的靶基因在4龄若蚜中的表达量均高于其在成虫中的表达量,表明miRNA对其靶基因的调控作用可能集中在成虫阶段。分析豌豆蚜有翅和无翅个体中5个miRNA的表达情况发现,在成虫有翅个体中5个miRNA的表达量均高于无翅个体中的,其中miR-277表达差异最显著,成虫有翅个体中的表达量是无翅个体中表达量的7.5倍;其次为Let-7,表达差异达3倍。而Let-7在3龄有翅若蚜和无翅若蚜中表达差异最显著,有翅个体中的表达量是无翅个体中的37.8倍;其次为miR-277,表达差异达7.6倍。比较5个miRNA与其靶基因在豌豆蚜3-4龄若蚜及成虫有翅和无翅个体中的表达发现,miRNA Let-7和miR-92b的表达趋势分别与其靶基因abrupt和Foxo的基本相反。荧光素酶活性检测结果显示,Let-7的真实靶标为abrupt,共转染Let-7模拟物后与对照相比,荧光素酶活性下降53%,达极显著水平。其他miRNA与靶标基因的互作不显著。【结论】首次发现miRNA对豌豆蚜孤雌蚜翅型分化相关基因的调控可能发生在成虫阶段。Let-7可能通过调控abrupt基因参与孤雌蚜翅型分化。该研究为进一步探索miRNA参与孤雌蚜翅两型性分化的机制奠定了基础。
Abstract
【mu de 】qian ji yan jiu fa xian mai chang guan ya Sitobion avenaegu ci ya you chi he mo chi ge ti zhong cun zai hen duo cha yi biao da de wei xiao RNA(microRNA, miRNA),ben yan jiu zhi zai jin yi bu ming que zhe xie miRNAzai wan dou ya Acyrthosiphon pisumzhong fa hui zuo yong de fa yo jie duan ,tan suo miRNAdiao kong gu ci ya chi liang xing xing fen hua de ji zhi 。【fang fa 】shua ze zai mai chang guan ya you chi ya he mo chi ya zhong xian zhe cha yi biao da ,ju ba ji yin wei tui pi ji su 、yi dao su xin hao tong lu ji chi xing fa yo guan jian ji yin de 5ge miRNA(Let-7,miR-92a,miR-92b,miR-92a-1-p5he miR-277),li yong qPCRjian ce zhe xie miRNAji ji ba biao ji yin zai wan dou ya 3-4ling re ya he cheng chong you chi he mo chi ge ti zhong de biao da pu ;tong shi li yong shuang ying guang su mei huo xing jian ce fa dui shang shu miRNAde ba ji yin jin hang yan zheng 。【jie guo 】biao da pu fen xi fa xian ,zhe 5ge miRNAzai wan dou ya cheng chong zhong biao da liang jun gao yu ji zai re ya zhong de biao da liang ,er ji yu ce de ba ji yin zai 4ling re ya zhong de biao da liang jun gao yu ji zai cheng chong zhong de biao da liang ,biao ming miRNAdui ji ba ji yin de diao kong zuo yong ke neng ji zhong zai cheng chong jie duan 。fen xi wan dou ya you chi he mo chi ge ti zhong 5ge miRNAde biao da qing kuang fa xian ,zai cheng chong you chi ge ti zhong 5ge miRNAde biao da liang jun gao yu mo chi ge ti zhong de ,ji zhong miR-277biao da cha yi zui xian zhe ,cheng chong you chi ge ti zhong de biao da liang shi mo chi ge ti zhong biao da liang de 7.5bei ;ji ci wei Let-7,biao da cha yi da 3bei 。er Let-7zai 3ling you chi re ya he mo chi re ya zhong biao da cha yi zui xian zhe ,you chi ge ti zhong de biao da liang shi mo chi ge ti zhong de 37.8bei ;ji ci wei miR-277,biao da cha yi da 7.6bei 。bi jiao 5ge miRNAyu ji ba ji yin zai wan dou ya 3-4ling re ya ji cheng chong you chi he mo chi ge ti zhong de biao da fa xian ,miRNA Let-7he miR-92bde biao da qu shi fen bie yu ji ba ji yin abrupthe Foxode ji ben xiang fan 。ying guang su mei huo xing jian ce jie guo xian shi ,Let-7de zhen shi ba biao wei abrupt,gong zhuai ran Let-7mo ni wu hou yu dui zhao xiang bi ,ying guang su mei huo xing xia jiang 53%,da ji xian zhe shui ping 。ji ta miRNAyu ba biao ji yin de hu zuo bu xian zhe 。【jie lun 】shou ci fa xian miRNAdui wan dou ya gu ci ya chi xing fen hua xiang guan ji yin de diao kong ke neng fa sheng zai cheng chong jie duan 。Let-7ke neng tong guo diao kong abruptji yin can yu gu ci ya chi xing fen hua 。gai yan jiu wei jin yi bu tan suo miRNAcan yu gu ci ya chi liang xing xing fen hua de ji zhi dian ding le ji chu 。
论文参考文献
论文详细介绍
论文作者分别是来自昆虫学报的杨宗霖,王艺,马田田,霍春月,刘晓,阚云超,李丹丹，发表于刊物昆虫学报2019年11期论文，是一篇关于豌豆蚜论文,孤雌生殖论文,翅型分化论文,靶基因论文,表达谱论文,昆虫学报2019年11期论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自昆虫学报2019年11期论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。