本文主要研究内容
作者赖瑞强（2019）在《烟草青枯病抗性的连锁与连锁不平衡联合作图》一文中研究指出：烟草青枯病是影响烟草产质量的主要病害之一,该病的发生常造成重大的经济损失。选育优良抗病品种是减少青枯病发生的最佳措施,而运用与抗病相关的分子标记辅助抗病材料的筛选能加速品种选育的进程。因此,了解青枯病抗病遗传特性,探测和发掘与青枯病抗性密切相关的优异等位基因,辅助烟草抗病材料的筛选,对提高烟草抗青枯病的能力至关重要。现阶段,青枯病抗病遗传分析主要采用连锁分析或关联分析;然而,烟草青枯病抗性属于数量性状遗传,单纯利用连锁分析或关联分析对其进行研究,探测到的信息有限。因此,本研究利用一个包含133份F7单粒传重组自交系（Recombinant inbred lines,RILs）群体和一个包含94份种质的自然群体分别进行连锁分析和关联分析,并利用共同的分子标记开展联合作图,探讨烟草青枯病的抗病遗传。主要研究结果如下:1、应用R语言对RIL群体和自然群体多年多点的青枯病病情指数进行主效可加互作可乘（Additive main effects and multiplicative interaction,AMMI）模型分析,获悉基因型和环境对供试烟草表型的作用力。结果发现环境因子对两个群体的烟草青枯病抗性的影响最大,其次为基因型,而基因型与环境的交互作用的影响效应最小。对各株系和各种质材料对青枯病抗性的稳定性进行评价,发现RIL群体的稳定性较自然群体的高;其中,RIL群体各株系的稳定性参数在0.0070.798之间,平均为0.510,株系稳定性具有明显差异,稳定性最好的材料为株系48;而自然群体各材料的稳定性参数在0.5320.619之间,平均为0.605,材料间稳定性差异不明显,稳定性最好的种质为“丰字6号”。2、利用253个SSRs和293个InDels共546个分子标记对133份RILs进行基因分型,继而构建了一张包含192个SSRs和271个InDels共463个标记的遗传连锁图谱。该图谱覆盖基因组总长度为1230.73 cM,标记平均间距为2.66 cM。据此,利用4个环境中RIL群体各株系的病情指数数据进行QTL定位,探测到4个QTLs,即qTBWR-6-E4、qTBWR-11-E3、qTBWR-17-E1和qTBWR-18-E2。这些QTLs的LOD值均大于3,且青枯病病情指数的表型变异解释率均超过10%,分别位于连锁群6、连锁群11、连锁群17和连锁群18。该结果丰富了青枯病抗性的QTL位点,为分子标记辅助育种积累了理论基础。3、利用546个分子标记对94份烟草种质组成的自然群体进行基因分型和群体结构分析,开展基于连锁不平衡的关联分析探测与青枯病抗性相关的分子标记。结果表明546个标记在94份烟草种质中检测到1503个等位变异位点,平均每个标记为2.75个位点;这些标记的多态性信息量（PIC）值在0.10070.8760之间,平均为0.4584。基于等位变异位点信息,构建了376个单倍型;在此基础上进行群体结构分析,将94份种质划分为2个类群,表明本研究烟草种质的群体结构简单,有利于减少伪关联。二年田间鉴定数据分别与376个单倍型和1503个标记位点进行关联分析,发现8个单倍型和17个等位位点对青枯病病情指数的表现变异解释率分别达到8.21%11.42%和7.91%23.31%。4、利用RIL群体和自然群体进行连锁与连锁不平衡联合作图分析,在平行作图分析中发现单倍型Hap227与qTBWR-18-E2在连锁群LG18上的距离仅1.14cM,而单倍型Hap363与qTBWR-17-E1在连锁群LG17的距离为3.15 cM,因此这两个区段可能存在青枯病抗性的相关基因。在整合作图中共发现到8个单倍型和10个等位变异与烟草青枯病抗病性相关,对青枯病病情指数的表现变异解释率分别在4.95%7.06%和3.49%6.27%之间。另外,在连锁分析中检测到的QTL qTBWR-6-E4其左翼标记RAD-66中的两个变异位点（亦为单倍型Hap’159）在整合作图分析中被探测到与抗病性相关;还有单倍型Hap’545、Hap’546（亦为单倍型Hap363）和变异位点S’1及S’3在94份材料的关联分析和整合作图分析中均被探测到与抗病性相关。5、检测经连锁分析、关联分析和整合作图分析其中两种或以上方法共同发现的与抗病性相关的4个单倍型（Hap’159、Hap’545、Hap’546、Hap227）和2个变异位点（S’1和S’3）的效应,发现Hap’159和Hap’546对青枯病病情指数具有减效效应,而Hap’545、Hap227、S’1和S’3为增效效应。利用这些单倍型和位点共同评价烟草材料,发现减效效应可达到-12.91,远高于单一单倍型或位点或它们间的其他不同组合评价的平均效应值,且其载体材料全部表现出抗性。通过分子标记结合稳定性参数,发现1个具有高抗且较稳定的重组自交系株系106,其稳定性参数为0.094,两年多点环境下的平均病情指数仅为5.56。研究结果可为烟草青枯病抗病材料的分子标记辅助筛选提供参考。
Abstract
yan cao qing ku bing shi ying xiang yan cao chan zhi liang de zhu yao bing hai zhi yi ,gai bing de fa sheng chang zao cheng chong da de jing ji sun shi 。shua yo you liang kang bing pin chong shi jian shao qing ku bing fa sheng de zui jia cuo shi ,er yun yong yu kang bing xiang guan de fen zi biao ji fu zhu kang bing cai liao de shai shua neng jia su pin chong shua yo de jin cheng 。yin ci ,le jie qing ku bing kang bing wei chuan te xing ,tan ce he fa jue yu qing ku bing kang xing mi qie xiang guan de you yi deng wei ji yin ,fu zhu yan cao kang bing cai liao de shai shua ,dui di gao yan cao kang qing ku bing de neng li zhi guan chong yao 。xian jie duan ,qing ku bing kang bing wei chuan fen xi zhu yao cai yong lian suo fen xi huo guan lian fen xi ;ran er ,yan cao qing ku bing kang xing shu yu shu liang xing zhuang wei chuan ,chan chun li yong lian suo fen xi huo guan lian fen xi dui ji jin hang yan jiu ,tan ce dao de xin xi you xian 。yin ci ,ben yan jiu li yong yi ge bao han 133fen F7chan li chuan chong zu zi jiao ji （Recombinant inbred lines,RILs）qun ti he yi ge bao han 94fen chong zhi de zi ran qun ti fen bie jin hang lian suo fen xi he guan lian fen xi ,bing li yong gong tong de fen zi biao ji kai zhan lian ge zuo tu ,tan tao yan cao qing ku bing de kang bing wei chuan 。zhu yao yan jiu jie guo ru xia :1、ying yong Ryu yan dui RILqun ti he zi ran qun ti duo nian duo dian de qing ku bing bing qing zhi shu jin hang zhu xiao ke jia hu zuo ke cheng （Additive main effects and multiplicative interaction,AMMI）mo xing fen xi ,huo xi ji yin xing he huan jing dui gong shi yan cao biao xing de zuo yong li 。jie guo fa xian huan jing yin zi dui liang ge qun ti de yan cao qing ku bing kang xing de ying xiang zui da ,ji ci wei ji yin xing ,er ji yin xing yu huan jing de jiao hu zuo yong de ying xiang xiao ying zui xiao 。dui ge zhu ji he ge chong zhi cai liao dui qing ku bing kang xing de wen ding xing jin hang ping jia ,fa xian RILqun ti de wen ding xing jiao zi ran qun ti de gao ;ji zhong ,RILqun ti ge zhu ji de wen ding xing can shu zai 0.0070.798zhi jian ,ping jun wei 0.510,zhu ji wen ding xing ju you ming xian cha yi ,wen ding xing zui hao de cai liao wei zhu ji 48;er zi ran qun ti ge cai liao de wen ding xing can shu zai 0.5320.619zhi jian ,ping jun wei 0.605,cai liao jian wen ding xing cha yi bu ming xian ,wen ding xing zui hao de chong zhi wei “feng zi 6hao ”。2、li yong 253ge SSRshe 293ge InDelsgong 546ge fen zi biao ji dui 133fen RILsjin hang ji yin fen xing ,ji er gou jian le yi zhang bao han 192ge SSRshe 271ge InDelsgong 463ge biao ji de wei chuan lian suo tu pu 。gai tu pu fu gai ji yin zu zong chang du wei 1230.73 cM,biao ji ping jun jian ju wei 2.66 cM。ju ci ,li yong 4ge huan jing zhong RILqun ti ge zhu ji de bing qing zhi shu shu ju jin hang QTLding wei ,tan ce dao 4ge QTLs,ji qTBWR-6-E4、qTBWR-11-E3、qTBWR-17-E1he qTBWR-18-E2。zhe xie QTLsde LODzhi jun da yu 3,ju qing ku bing bing qing zhi shu de biao xing bian yi jie shi lv jun chao guo 10%,fen bie wei yu lian suo qun 6、lian suo qun 11、lian suo qun 17he lian suo qun 18。gai jie guo feng fu le qing ku bing kang xing de QTLwei dian ,wei fen zi biao ji fu zhu yo chong ji lei le li lun ji chu 。3、li yong 546ge fen zi biao ji dui 94fen yan cao chong zhi zu cheng de zi ran qun ti jin hang ji yin fen xing he qun ti jie gou fen xi ,kai zhan ji yu lian suo bu ping heng de guan lian fen xi tan ce yu qing ku bing kang xing xiang guan de fen zi biao ji 。jie guo biao ming 546ge biao ji zai 94fen yan cao chong zhi zhong jian ce dao 1503ge deng wei bian yi wei dian ,ping jun mei ge biao ji wei 2.75ge wei dian ;zhe xie biao ji de duo tai xing xin xi liang （PIC）zhi zai 0.10070.8760zhi jian ,ping jun wei 0.4584。ji yu deng wei bian yi wei dian xin xi ,gou jian le 376ge chan bei xing ;zai ci ji chu shang jin hang qun ti jie gou fen xi ,jiang 94fen chong zhi hua fen wei 2ge lei qun ,biao ming ben yan jiu yan cao chong zhi de qun ti jie gou jian chan ,you li yu jian shao wei guan lian 。er nian tian jian jian ding shu ju fen bie yu 376ge chan bei xing he 1503ge biao ji wei dian jin hang guan lian fen xi ,fa xian 8ge chan bei xing he 17ge deng wei wei dian dui qing ku bing bing qing zhi shu de biao xian bian yi jie shi lv fen bie da dao 8.21%11.42%he 7.91%23.31%。4、li yong RILqun ti he zi ran qun ti jin hang lian suo yu lian suo bu ping heng lian ge zuo tu fen xi ,zai ping hang zuo tu fen xi zhong fa xian chan bei xing Hap227yu qTBWR-18-E2zai lian suo qun LG18shang de ju li jin 1.14cM,er chan bei xing Hap363yu qTBWR-17-E1zai lian suo qun LG17de ju li wei 3.15 cM,yin ci zhe liang ge ou duan ke neng cun zai qing ku bing kang xing de xiang guan ji yin 。zai zheng ge zuo tu zhong gong fa xian dao 8ge chan bei xing he 10ge deng wei bian yi yu yan cao qing ku bing kang bing xing xiang guan ,dui qing ku bing bing qing zhi shu de biao xian bian yi jie shi lv fen bie zai 4.95%7.06%he 3.49%6.27%zhi jian 。ling wai ,zai lian suo fen xi zhong jian ce dao de QTL qTBWR-6-E4ji zuo yi biao ji RAD-66zhong de liang ge bian yi wei dian （yi wei chan bei xing Hap’159）zai zheng ge zuo tu fen xi zhong bei tan ce dao yu kang bing xing xiang guan ;hai you chan bei xing Hap’545、Hap’546（yi wei chan bei xing Hap363）he bian yi wei dian S’1ji S’3zai 94fen cai liao de guan lian fen xi he zheng ge zuo tu fen xi zhong jun bei tan ce dao yu kang bing xing xiang guan 。5、jian ce jing lian suo fen xi 、guan lian fen xi he zheng ge zuo tu fen xi ji zhong liang chong huo yi shang fang fa gong tong fa xian de yu kang bing xing xiang guan de 4ge chan bei xing （Hap’159、Hap’545、Hap’546、Hap227）he 2ge bian yi wei dian （S’1he S’3）de xiao ying ,fa xian Hap’159he Hap’546dui qing ku bing bing qing zhi shu ju you jian xiao xiao ying ,er Hap’545、Hap227、S’1he S’3wei zeng xiao xiao ying 。li yong zhe xie chan bei xing he wei dian gong tong ping jia yan cao cai liao ,fa xian jian xiao xiao ying ke da dao -12.91,yuan gao yu chan yi chan bei xing huo wei dian huo ta men jian de ji ta bu tong zu ge ping jia de ping jun xiao ying zhi ,ju ji zai ti cai liao quan bu biao xian chu kang xing 。tong guo fen zi biao ji jie ge wen ding xing can shu ,fa xian 1ge ju you gao kang ju jiao wen ding de chong zu zi jiao ji zhu ji 106,ji wen ding xing can shu wei 0.094,liang nian duo dian huan jing xia de ping jun bing qing zhi shu jin wei 5.56。yan jiu jie guo ke wei yan cao qing ku bing kang bing cai liao de fen zi biao ji fu zhu shai shua di gong can kao 。
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论文作者分别是来自广州大学的赖瑞强，发表于刊物广州大学2019-10-11论文，是一篇关于烟草论文,青枯病论文,连锁分析论文,关联分析论文,连锁和连锁不平衡联合分析论文,广州大学2019-10-11论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自广州大学2019-10-11论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：烟草论文; 青枯病论文; 连锁分析论文; 关联分析论文; 连锁和连锁不平衡联合分析论文; 广州大学2019-10-11论文;