本文主要研究内容
作者叶文（2019）在《自体动静脉内瘘流出道静脉狭窄组织中非编码RNA的表达谱及生物信息学分析》一文中研究指出：目的:通过高通量测序(High-throughput sequencing)检测肾衰竭患者自体动静脉内瘘(autologous arteriovenous fistula,AVF)流出道静脉狭窄组织中长链非编码RNA(long noncoding RNA,lncRNA)的表达谱变化,探讨lncRNA与AVF流出道静脉狭窄的关系。方法:选择2017年2至6月在南昌大学第一附属医院肾内科诊断AVF失功患者再次手术的血管组织28例(实验组),纳入标准为内瘘使用时间≥3个月;内瘘透析时血流量≤200 ml/min;内瘘狭窄程度大于50%,排除有明显血栓形成者;上肢血管超声检查资料完整者。对照组选自同一机构尿毒症患者初次行动静脉内瘘术的血管组织75例,纳入标准为流出道血管内径≥1.6 mm,且无血管狭窄或其他血管疾病者。将103个样本采用Trizol法提取总RNA,分别选取实验组和对照组各4例,利用高通量测序分别检测其lncRNA及mRNA表达谱的差异,随后筛选出差异表达的lncRNA及mRNA,进行聚类分析,GO功能分析、KEGG pathway分析;并用实时荧光定量PCR(real-time RT-PCR,qRT-PCR)对RNA-seq结果进行验证;最后扩大样本量验证LINC01615在动静脉内瘘流出道静脉狭窄组织中高表达。结果:与对照组相比,表达变化≥2倍并且差异有统计学意义(P<0.05)的lncRNA或mRNA,认为是显著差异表达的lncRNA或mRNA。(1)lncRNA表达变化≥2倍的共247个,实验组表达上调≥2倍的有141个,表达下调≥2倍的有106个;(2)mRNA表达变化≥2倍的共1386个,实验组表达上调≥2倍的有874个,表达下调≥2倍的有512个;(3)GO功能分析提示显著差异表达的lncRNA主要参与了细胞的分化、凋亡;血管形态发生、血管发育、脉管系统发育等;(4)KEGG pathway分析提示差异表达的基因注释到PI3K-AKT信号通路是最多的,其显著性最高的是TGF-β信号通路。(5)在显著差异表达的ncRNA中,使用qRT-PCR技术对关键性的9个lncRNA及7个mRNA表达水平进行验证,其结果与测序一致;(6)与对照组相比,LINC01615在动静脉内瘘流出道静脉狭窄组织中高表达。结论:尿毒症血液透析患者动静脉内瘘流出道静脉组织存在显著差异性表达的lncRNA,这些lncRNA及某些信号通路可能在尿毒症患者AVF流出道静脉狭窄发生和发展中发挥重要作用。本研究可能为AVF流出道狭窄的发病机制的阐述奠定基础,并为AVF失功患者提供潜在的预防靶点。
Abstract
mu de :tong guo gao tong liang ce xu (High-throughput sequencing)jian ce shen cui jie huan zhe zi ti dong jing mai nei lou (autologous arteriovenous fistula,AVF)liu chu dao jing mai xia zhai zu zhi zhong chang lian fei bian ma RNA(long noncoding RNA,lncRNA)de biao da pu bian hua ,tan tao lncRNAyu AVFliu chu dao jing mai xia zhai de guan ji 。fang fa :shua ze 2017nian 2zhi 6yue zai na chang da xue di yi fu shu yi yuan shen nei ke zhen duan AVFshi gong huan zhe zai ci shou shu de xie guan zu zhi 28li (shi yan zu ),na ru biao zhun wei nei lou shi yong shi jian ≥3ge yue ;nei lou tou xi shi xie liu liang ≤200 ml/min;nei lou xia zhai cheng du da yu 50%,pai chu you ming xian xie shuan xing cheng zhe ;shang zhi xie guan chao sheng jian cha zi liao wan zheng zhe 。dui zhao zu shua zi tong yi ji gou niao du zheng huan zhe chu ci hang dong jing mai nei lou shu de xie guan zu zhi 75li ,na ru biao zhun wei liu chu dao xie guan nei jing ≥1.6 mm,ju mo xie guan xia zhai huo ji ta xie guan ji bing zhe 。jiang 103ge yang ben cai yong Trizolfa di qu zong RNA,fen bie shua qu shi yan zu he dui zhao zu ge 4li ,li yong gao tong liang ce xu fen bie jian ce ji lncRNAji mRNAbiao da pu de cha yi ,sui hou shai shua chu cha yi biao da de lncRNAji mRNA,jin hang ju lei fen xi ,GOgong neng fen xi 、KEGG pathwayfen xi ;bing yong shi shi ying guang ding liang PCR(real-time RT-PCR,qRT-PCR)dui RNA-seqjie guo jin hang yan zheng ;zui hou kuo da yang ben liang yan zheng LINC01615zai dong jing mai nei lou liu chu dao jing mai xia zhai zu zhi zhong gao biao da 。jie guo :yu dui zhao zu xiang bi ,biao da bian hua ≥2bei bing ju cha yi you tong ji xue yi yi (P<0.05)de lncRNAhuo mRNA,ren wei shi xian zhe cha yi biao da de lncRNAhuo mRNA。(1)lncRNAbiao da bian hua ≥2bei de gong 247ge ,shi yan zu biao da shang diao ≥2bei de you 141ge ,biao da xia diao ≥2bei de you 106ge ;(2)mRNAbiao da bian hua ≥2bei de gong 1386ge ,shi yan zu biao da shang diao ≥2bei de you 874ge ,biao da xia diao ≥2bei de you 512ge ;(3)GOgong neng fen xi di shi xian zhe cha yi biao da de lncRNAzhu yao can yu le xi bao de fen hua 、diao wang ;xie guan xing tai fa sheng 、xie guan fa yo 、mai guan ji tong fa yo deng ;(4)KEGG pathwayfen xi di shi cha yi biao da de ji yin zhu shi dao PI3K-AKTxin hao tong lu shi zui duo de ,ji xian zhe xing zui gao de shi TGF-βxin hao tong lu 。(5)zai xian zhe cha yi biao da de ncRNAzhong ,shi yong qRT-PCRji shu dui guan jian xing de 9ge lncRNAji 7ge mRNAbiao da shui ping jin hang yan zheng ,ji jie guo yu ce xu yi zhi ;(6)yu dui zhao zu xiang bi ,LINC01615zai dong jing mai nei lou liu chu dao jing mai xia zhai zu zhi zhong gao biao da 。jie lun :niao du zheng xie ye tou xi huan zhe dong jing mai nei lou liu chu dao jing mai zu zhi cun zai xian zhe cha yi xing biao da de lncRNA,zhe xie lncRNAji mou xie xin hao tong lu ke neng zai niao du zheng huan zhe AVFliu chu dao jing mai xia zhai fa sheng he fa zhan zhong fa hui chong yao zuo yong 。ben yan jiu ke neng wei AVFliu chu dao xia zhai de fa bing ji zhi de chan shu dian ding ji chu ,bing wei AVFshi gong huan zhe di gong qian zai de yu fang ba dian 。
论文参考文献
论文详细介绍
论文作者分别是来自南昌大学的叶文，发表于刊物南昌大学2019-09-12论文，是一篇关于自体动静脉内瘘论文,长链非编码论文,测序论文,南昌大学2019-09-12论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自南昌大学2019-09-12论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：自体动静脉内瘘论文; 长链非编码论文; 测序论文; 南昌大学2019-09-12论文;