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作者赵艳（2019）在《不同分子量褐藻酸钠对Cd2+、Pb2+所致斑马鱼胚胎发育毒性的保护作用》一文中研究指出：褐藻酸钠是一种从海带、紫菜等水生大型藻类中提取的多聚糖,广泛应用于食品、药品、化工业等多个领域。目前证实褐藻酸钠具有广泛生理功能,且发现分子量大小影响其活性。本文探讨以酸水解和/或氧化工艺降解褐藻酸,获得不同分子量范围的褐藻多糖,并考察其对Cd2+、Pb2+暴露所致斑马鱼胚胎发育毒性的保护作用,为其应用开发提供依据。（1）分别采用盐酸（HCl）、过氧化氢（H2O2）单独以及两者联用降解褐藻酸钠,并通过粘度法考察溶液粘度表征降解效果。研究发现,延长降解时间不能改善H2O2降解效果,可明显改善HCl降解效果;HCl-H2O2联用可加速褐藻酸钠降解。红外光谱法测定褐藻酸钠的官能团表明,降解后褐藻酸钠单元结构未发生明显变化,不同降解条件仅打断褐藻酸内部糖苷键,形成低分子量多聚糖。通过葡聚糖G-50凝胶柱分离降解后褐藻酸钠,采用HPLC-ELSD法检测分离产物分子量,获得了平均分子量分别为1575 D、7075 D、61518 D的三种多糖组分。（2）采用受精后6小时（6 hpf）斑马鱼卵,暴露于含氯化镉(Cd2+)和硝酸铅(Pb2+)的孵育液建立Cd2+和Pb2+染毒的发育毒性评价模型。结果表明,随着Cd2+、Pb2+浓度增加,斑马鱼胚胎的死亡率和畸形率升高,孵化率降低,两者均表现出明显的发育毒性。其中氯化镉对斑马鱼胚胎的半数致死浓度(LC50)为15.86 mg/L,硝酸铅的半数致死浓度(LC50)为125 mg/L。采用分离得到的不同分子量褐藻酸钠与Cd2+、Pb2+联合作用于斑马鱼胚胎,结果表明,1575 D、7075 D和61518 D平均分子量的褐藻酸钠对镉处理致斑马鱼胚胎死亡最强保护作用浓度分别为5 mg/L、10 mg/L和50 mg/L;仅1575 D褐藻酸对于Pb2+诱导的斑马鱼发育毒性有保护作用,最佳保护浓度为25 mg/L,7075 D和61518 D褐藻酸钠未见明显保护作用。（3）采用吖啶橙（AO）染色法观察胚胎凋亡,DCFH-DA探针法测定胚胎活性氧（ROS）水平,TBA比色法测定脂质过氧化物丙二醛（MDA）水平和超氧化物歧化酶（SOD）活性,ICP-MS法测定全卵和完整胚胎内Cd2+、Pb2+含量。结果表明,Cd2+和Pb2+均可诱导斑马鱼胚胎细胞凋亡,并致胚胎MDA和ROS水平上升,SOD活性下降。不同分子量褐藻酸钠均可降低Cd2+于斑马鱼全卵和胚胎内的富集,并改善Cd2+所致胚胎细胞凋亡,降低MDA和ROS水平,升高SOD活性,尤以低分子量褐藻酸活性最强。低分子量褐藻酸对Pb2+所致斑马鱼胚胎发育毒性有相似干预作用。研究结果表明,Cd2+和Pb2+均能诱导斑马鱼胚胎发育毒性和氧化应激损伤,降解后1575 D低分子量褐藻酸钠可明显改善Cd2+和Pb2+在斑马鱼卵和胚胎内的蓄积,并明显改善胚胎氧化应激水平,减少细胞凋亡,防护Cd2+和Pb2+所致斑马鱼胚胎发育毒性。
Abstract
he zao suan na shi yi chong cong hai dai 、zi cai deng shui sheng da xing zao lei zhong di qu de duo ju tang ,an fan ying yong yu shi pin 、yao pin 、hua gong ye deng duo ge ling yu 。mu qian zheng shi he zao suan na ju you an fan sheng li gong neng ,ju fa xian fen zi liang da xiao ying xiang ji huo xing 。ben wen tan tao yi suan shui jie he /huo yang hua gong yi jiang jie he zao suan ,huo de bu tong fen zi liang fan wei de he zao duo tang ,bing kao cha ji dui Cd2+、Pb2+bao lou suo zhi ban ma yu pei tai fa yo du xing de bao hu zuo yong ,wei ji ying yong kai fa di gong yi ju 。（1）fen bie cai yong yan suan （HCl）、guo yang hua qing （H2O2）chan du yi ji liang zhe lian yong jiang jie he zao suan na ,bing tong guo nian du fa kao cha rong ye nian du biao zheng jiang jie xiao guo 。yan jiu fa xian ,yan chang jiang jie shi jian bu neng gai shan H2O2jiang jie xiao guo ,ke ming xian gai shan HCljiang jie xiao guo ;HCl-H2O2lian yong ke jia su he zao suan na jiang jie 。gong wai guang pu fa ce ding he zao suan na de guan neng tuan biao ming ,jiang jie hou he zao suan na chan yuan jie gou wei fa sheng ming xian bian hua ,bu tong jiang jie tiao jian jin da duan he zao suan nei bu tang gan jian ,xing cheng di fen zi liang duo ju tang 。tong guo pu ju tang G-50ning jiao zhu fen li jiang jie hou he zao suan na ,cai yong HPLC-ELSDfa jian ce fen li chan wu fen zi liang ,huo de le ping jun fen zi liang fen bie wei 1575 D、7075 D、61518 Dde san chong duo tang zu fen 。（2）cai yong shou jing hou 6xiao shi （6 hpf）ban ma yu luan ,bao lou yu han lv hua ge (Cd2+)he xiao suan qian (Pb2+)de fu yo ye jian li Cd2+he Pb2+ran du de fa yo du xing ping jia mo xing 。jie guo biao ming ,sui zhao Cd2+、Pb2+nong du zeng jia ,ban ma yu pei tai de si wang lv he ji xing lv sheng gao ,fu hua lv jiang di ,liang zhe jun biao xian chu ming xian de fa yo du xing 。ji zhong lv hua ge dui ban ma yu pei tai de ban shu zhi si nong du (LC50)wei 15.86 mg/L,xiao suan qian de ban shu zhi si nong du (LC50)wei 125 mg/L。cai yong fen li de dao de bu tong fen zi liang he zao suan na yu Cd2+、Pb2+lian ge zuo yong yu ban ma yu pei tai ,jie guo biao ming ,1575 D、7075 Dhe 61518 Dping jun fen zi liang de he zao suan na dui ge chu li zhi ban ma yu pei tai si wang zui jiang bao hu zuo yong nong du fen bie wei 5 mg/L、10 mg/Lhe 50 mg/L;jin 1575 Dhe zao suan dui yu Pb2+you dao de ban ma yu fa yo du xing you bao hu zuo yong ,zui jia bao hu nong du wei 25 mg/L,7075 Dhe 61518 Dhe zao suan na wei jian ming xian bao hu zuo yong 。（3）cai yong a ding cheng （AO）ran se fa guan cha pei tai diao wang ,DCFH-DAtan zhen fa ce ding pei tai huo xing yang （ROS）shui ping ,TBAbi se fa ce ding zhi zhi guo yang hua wu bing er quan （MDA）shui ping he chao yang hua wu qi hua mei （SOD）huo xing ,ICP-MSfa ce ding quan luan he wan zheng pei tai nei Cd2+、Pb2+han liang 。jie guo biao ming ,Cd2+he Pb2+jun ke you dao ban ma yu pei tai xi bao diao wang ,bing zhi pei tai MDAhe ROSshui ping shang sheng ,SODhuo xing xia jiang 。bu tong fen zi liang he zao suan na jun ke jiang di Cd2+yu ban ma yu quan luan he pei tai nei de fu ji ,bing gai shan Cd2+suo zhi pei tai xi bao diao wang ,jiang di MDAhe ROSshui ping ,sheng gao SODhuo xing ,you yi di fen zi liang he zao suan huo xing zui jiang 。di fen zi liang he zao suan dui Pb2+suo zhi ban ma yu pei tai fa yo du xing you xiang shi gan yu zuo yong 。yan jiu jie guo biao ming ,Cd2+he Pb2+jun neng you dao ban ma yu pei tai fa yo du xing he yang hua ying ji sun shang ,jiang jie hou 1575 Ddi fen zi liang he zao suan na ke ming xian gai shan Cd2+he Pb2+zai ban ma yu luan he pei tai nei de xu ji ,bing ming xian gai shan pei tai yang hua ying ji shui ping ,jian shao xi bao diao wang ,fang hu Cd2+he Pb2+suo zhi ban ma yu pei tai fa yo du xing 。
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论文作者分别是来自烟台大学的赵艳，发表于刊物烟台大学2019-08-29论文，是一篇关于褐藻酸钠论文,降解条件论文,斑马鱼论文,发育毒性论文,氧化应激论文,烟台大学2019-08-29论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自烟台大学2019-08-29论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。