论文摘要
目的1.肾间质纤维化是多种肾脏疾病走向肾功能衰竭的共同病理过程。其发病机制迄今为止仍未得到确切的阐明。通常认为是多因素综合作用的结果。其中转化生长因子（Transforming growth factor-β,TGF-β）作为一种强效的致纤维化因子,在肾间质纤维化发生、发展的多个环节起作用,被认为是肾纤维化最重要的作用因子之一。Smads家族蛋白是新近发现的参与TGF-β超家族在细胞内信号传导的一族蛋白质,是TGF-β超家族成员参与生命活动的重要介质。本研究应用单侧输尿管结扎术（unilateral ureteral obstruction ,UUO）制作肾间质纤维化模型,并用体外人重组TGF-β1刺激人肾近端小管上皮细胞（human kidney proximal tubular epithelial cell, HKC）,从整体、细胞和分子等不同水平,系统观察TGF-?/Smads信号传导途径在肾间质纤维化时细胞外基质的形成、细胞凋亡和增殖中的作用。2.他汀类药物在高脂血症治疗中的作用已被得到普遍的认可。大量实验和临床研究提示,他汀类药物对肾脏具有直接保护作用,能延缓肾脏病变的进展,且与其降脂作用无关。其可能机制包括调节增殖/凋亡平衡、炎性细胞因子的产生及细胞内信号传导通路。另外,肾素-血管紧张素-醛固酮系统（rennin angiotensin system, RAS）尤其是血管紧张素II（Ang II）,在血流动力学调节和在肾硬化以及纤维化中起重要作用。Ang II是通过与其受体结合而进一步发挥作用的。缬沙坦（Valsartan）是近年来发现的一种血管紧张素II 1型受体拮抗剂（ang II type 1 receptor antagonism,AT1RA）,从受体水平上阻断Ang II的生物学作用。它不但具有肯定且稳定的降压作用,而且对靶器官的保护作用也是独一无二的。在本实验中,我们分别选用辛伐他汀和缬沙坦进行干预研究,观察这些药物对TGF-β1、Smad2/3、p- Smad2/3和Smad7以及I型胶原蛋白和/或mRNA表达的影响,以探讨辛伐他汀和缬沙坦的肾脏保护作用机制。方法
论文目录
相关论文文献
- [1].Regulatory Effect of Shenge Yifei Capsule on TGF-β1/Smad Signaling Pathway in Rats with Chronic Obstructive Pulmonary Disease[J]. Medicinal Plant 2020(01)
- [2].Linggui Zhugan Decoction(苓桂术甘汤) Inhibits Ventricular Remodeling after Acute Myocardial Infarction in Rats by Suppressing TGF-β1/Smad Signaling Pathway[J]. Chinese Journal of Integrative Medicine 2020(05)
- [3].TGFβ/Smad信号通路在口腔鳞癌热疗中的表达变化[J]. 实用口腔医学杂志 2019(01)
- [4].Wulong Xiaozheng Wan medicated serum inhibits epithelial-mesenchymal transition in human gastric carcinoma cell line BGC823 by modulation of transforming growth factor-β_1/Smad signaling[J]. Journal of Traditional Chinese Medicine 2019(03)
- [5].Involvement of Rho-associated coiled-coil kinase signaling inhibition in TGF-β1/Smad2,3 signal transduction in vitro[J]. International Journal of Ophthalmology 2017(12)
- [6].The Analysis of Relationship between MSTN/Smad Signal Path and Turpan Black Sheep Meat Production[J]. Agricultural Science & Technology 2016(01)
- [7].Association of Down-regulation of CD109 Expression with Up-expression of Smad7 in Pathogenesis of Psoriasis[J]. Journal of Huazhong University of Science and Technology(Medical Sciences) 2016(01)
- [8].脑星形细胞瘤组织TGF-β1、Smad7与VEGF的表达及意义[J]. 健康之路 2016(12)
- [9].Qingxuan Jiangya Decoction(清眩降压汤) Prevents Blood Pressure Elevation and Ameliorates Vascular Structural Remodeling via Modulating TGF-β 1/Smad Pathway in Spontaneously Hypertensive Rats[J]. Chinese Journal of Integrative Medicine 2020(03)
- [10].Early intervention with Di-Dang Decoction prevents macrovascular fibrosis in diabetic rats by regulating the TGF-β1/Smad signalling pathway[J]. Chinese Journal of Natural Medicines 2020(08)
- [11].Naringenin prevents experimental liver fibrosis by blocking TGFβ-Smad3 and JNK-Smad3 pathways[J]. World Journal of Gastroenterology 2017(24)
- [12].Anterior segment dysgenesis correlation with epithelial-mesenchymal transition in Smad4 knockout mice[J]. International Journal of Ophthalmology 2016(07)
- [13].Effect of bone marrow mesenchymal stem cells on the Smad expression of hepatic fibrosis rats[J]. Asian Pacific Journal of Tropical Medicine 2014(04)
- [14].Expression of USP15, TβR-Ⅰand Smad7 in Psoriasis[J]. Journal of Huazhong University of Science and Technology(Medical Sciences) 2014(03)
- [15].Smad蛋白研究进展[J]. 中国医学创新 2013(12)
- [16].Effect of the Protease Inhibitor MG132 on the Transforming Growth Factor-β/Smad Signaling Pathway in HSC-T6 Cells[J]. Journal of Huazhong University of Science and Technology(Medical Sciences) 2013(04)
- [17].Role of Smad7 in inflammatory bowel diseases[J]. World Journal of Gastroenterology 2012(40)
- [18].HSP70 decreases receptor-dependent phosphorylation of Smad2 and blocks TGF-β-induced epithelial-mesenchymal transition[J]. 遗传学报 2011(03)
- [19].Berbamine inhibits proliferation and induces apoptosis of KU812 cells by increasing Smad3 activity[J]. Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011(07)
- [20].Smad信号调控软骨形成机制的研究进展[J]. 国际口腔医学杂志 2010(05)
- [21].Smad7 dependent expression signature highlights BMP2 and HK2 signaling in HSC transdifferentiation[J]. World Journal of Gastroenterology 2010(41)
- [22].Smad蛋白在转化生长因子-β信号转导通路中的作用与机制[J]. 医学分子生物学杂志 2008(04)
- [23].微小RNA-21-5p靶向Smad7调控转化生长因子-β_1诱导心肌纤维化促进房颤的发生[J]. 临床内科杂志 2019(07)
- [24].孕期尼古丁暴露所致子代成骨细胞BMP-4/Smad信号通路的变化[J]. 医学研究杂志 2018(11)
- [25].白细胞介素37/Smad3在兔腹主动脉粥样硬化斑块中的表达研究[J]. 中国心血管杂志 2019(02)
- [26].三步序贯法对激素依赖型哮喘患者TGF-β_1/Smad信号通路及肺功能的影响[J]. 中华中医药杂志 2019(09)
- [27].牦牛bta-miR-1434-5p和Smad1基因组织表达谱及荧光素酶报告载体构建[J]. 中国农业大学学报 2018(05)
- [28].Effect of Banxia Xiexin decoction on Helicobacter pylori-related peptic ulcers and its possible mechanism via the TGF-β/Smad signaling pathway[J]. Journal of Traditional Chinese Medicine 2018(03)
- [29].San-Cao Granule (三草颗粒) Ameliorates Hepatic Fibrosis through High Mobility Group Box-1 Protein/Smad Signaling Pathway[J]. Chinese Journal of Integrative Medicine 2018(07)
- [30].Smad1基因在敖汉细毛羊发情不同时期卵巢中的表达规律研究[J]. 黑龙江畜牧兽医 2017(21)