本文主要研究内容
作者朱启良,刘洪凯,陈旭,董玉峰,王延平（2019）在《杨树人工林根际古菌群落随细根生长的演变》一文中研究指出：研究细根不同生长时期根际土壤古菌群落组成结构差异,对深入了解林木细根与土壤微生物互作关系具有重要理论意义.依据细根表面颜色,采集杨树一级细根不同生长时期(白色新生根、黄色成熟根、褐色衰老根)根际土壤并提取微生物总DNA,采用特异性引物对古菌16S rDNA V4-V5区进行扩增,利用Illumina MiSeq平台进行古菌高通量测序分析.结果表明:新生根和衰老根根际土壤古菌群落操作分类单元(OTU)丰富度相似,而成熟根根际土壤古菌群落OTU数量较少.新生根和成熟根根际土壤共同含有134个OTU;成熟根和衰老根根际土壤共同含有87个OTU,新生根和衰老根根际土壤共同拥有90个OTU.α多样性分析表明,成熟根根际土壤古菌群落Chao1指数和ACE指数显著低于新生根和衰老根根际土壤,而衰老根根际土壤古菌群落Simpson指数和Shannon指数显著低于新生根和成熟根根际土壤.PERMANOVA分析表明,新生根和衰老根根际土壤古菌群落组成有显著差异.物种注释显示,杨树根际土壤共包含12个古菌属,其中新生根5个、成熟根10个、衰老根6个.β多样性指数表明,杨树根际土壤古菌群落相似度随着细根的生长逐渐下降,不同生长阶段细根根际土壤的古菌群落结构有较大差异.其中,占绝对优势的古菌为氨氧化古菌Candidatus_Nitrososphaera,其相对丰度超过70%.且随细根生长发育,该类古菌在根际土壤中的丰度呈现上升趋势,表明其可能与细根的生长发育关系密切.
Abstract
yan jiu xi gen bu tong sheng chang shi ji gen ji tu rang gu jun qun la zu cheng jie gou cha yi ,dui shen ru le jie lin mu xi gen yu tu rang wei sheng wu hu zuo guan ji ju you chong yao li lun yi yi .yi ju xi gen biao mian yan se ,cai ji yang shu yi ji xi gen bu tong sheng chang shi ji (bai se xin sheng gen 、huang se cheng shou gen 、he se cui lao gen )gen ji tu rang bing di qu wei sheng wu zong DNA,cai yong te yi xing yin wu dui gu jun 16S rDNA V4-V5ou jin hang kuo zeng ,li yong Illumina MiSeqping tai jin hang gu jun gao tong liang ce xu fen xi .jie guo biao ming :xin sheng gen he cui lao gen gen ji tu rang gu jun qun la cao zuo fen lei chan yuan (OTU)feng fu du xiang shi ,er cheng shou gen gen ji tu rang gu jun qun la OTUshu liang jiao shao .xin sheng gen he cheng shou gen gen ji tu rang gong tong han you 134ge OTU;cheng shou gen he cui lao gen gen ji tu rang gong tong han you 87ge OTU,xin sheng gen he cui lao gen gen ji tu rang gong tong yong you 90ge OTU.αduo yang xing fen xi biao ming ,cheng shou gen gen ji tu rang gu jun qun la Chao1zhi shu he ACEzhi shu xian zhe di yu xin sheng gen he cui lao gen gen ji tu rang ,er cui lao gen gen ji tu rang gu jun qun la Simpsonzhi shu he Shannonzhi shu xian zhe di yu xin sheng gen he cheng shou gen gen ji tu rang .PERMANOVAfen xi biao ming ,xin sheng gen he cui lao gen gen ji tu rang gu jun qun la zu cheng you xian zhe cha yi .wu chong zhu shi xian shi ,yang shu gen ji tu rang gong bao han 12ge gu jun shu ,ji zhong xin sheng gen 5ge 、cheng shou gen 10ge 、cui lao gen 6ge .βduo yang xing zhi shu biao ming ,yang shu gen ji tu rang gu jun qun la xiang shi du sui zhao xi gen de sheng chang zhu jian xia jiang ,bu tong sheng chang jie duan xi gen gen ji tu rang de gu jun qun la jie gou you jiao da cha yi .ji zhong ,zhan jue dui you shi de gu jun wei an yang hua gu jun Candidatus_Nitrososphaera,ji xiang dui feng du chao guo 70%.ju sui xi gen sheng chang fa yo ,gai lei gu jun zai gen ji tu rang zhong de feng du cheng xian shang sheng qu shi ,biao ming ji ke neng yu xi gen de sheng chang fa yo guan ji mi qie .
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论文作者分别是来自应用生态学报的朱启良,刘洪凯,陈旭,董玉峰,王延平，发表于刊物应用生态学报2019年03期论文，是一篇关于细根生长论文,古菌群落论文,根际土壤论文,高通量测序论文,杨树人工林论文,应用生态学报2019年03期论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自应用生态学报2019年03期论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：细根生长论文; 古菌群落论文; 根际土壤论文; 高通量测序论文; 杨树人工林论文; 应用生态学报2019年03期论文;