本文主要研究内容
作者王莹（2019）在《链霉菌NEAU6固体发酵条件优化及其对四种蔬菜的促生作用研究》一文中研究指出：农药化肥的过度使用会引发严重的农业生态污染和食品安全问题,以益生菌为核心的微生物肥料不仅能够有效促进作物生长、改善作物品质,而且不会对土壤生态环境造成威胁。相比于传统液体发酵工艺,固体发酵工艺具有设备简单、成本低、污染少等优点,适于在农业领域应用。因此,通过固体发酵技术研发适用于农业生产的生物菌肥将为农业生产发展带来新机遇。本研究以具有促生作用的链霉菌NEAU6为研究对象,通过固体发酵方式进行培养,对其发酵基质进行筛选,并采用响应面分析法对固体发酵的培养条件进行优化。利用平板发芽实验和盆栽实验探究链霉菌NEAU6对生菜生长的促进效果,通过田间蔬菜种植实验,进一步探究了链霉菌NEAU6的最佳施用浓度,及其对蔬菜品质和叶绿素含量的影响,并研究了菌株在生菜根际的定殖能力,旨在为链霉菌NEAU6生物菌肥的工业化生产提供理论依据。同时探究了链霉菌NEAU6对土壤微生物种群数量的影响,在此过程中筛选出两株放线菌新种,并进行了多相分类学鉴定。本研究结果如下:（1）通过固体发酵基质筛选,确定链霉菌NEAU6的最佳固体发酵基质为蚯蚓粪和麦麸,比例为3:1。采用响应面分析法对链霉菌NEAU6的固体发酵培养条件进行优化,最佳发酵条件为接种量10%,含水量70%,温度27°C,发酵7天时,链霉菌NEAU6的孢子量达到最大,为6.05×1011CFU·g-1。（2）生菜种子平板发芽实验表明,链霉菌NEAU6能够显著提高生菜种子的芽长和根长,当孢子浓度为5.2×106CFU·mL-1时,芽长提高了27.78%,根长提高了14.71%。（3）生菜盆栽实验表明,当链霉菌NEAU6孢子量为6×107CFU·g-1时,生菜单株产量提高了26.00%,株高提高了12.35%,叶长和叶宽分别提高了8.00%和15.60%。（4）田间试验表明,当链霉菌NEAU6孢子量为6×107CFU·g-1时,显著的促进了生菜和小白菜的生长,与对照组相比,单株产量分别增加了48.00%和37.06%。当链霉菌NEAU6孢子量为6×106CFU·g-1时,对茼蒿和香菜的促生效果显著,与对照组相比,单株产量分别增加了40.84%和20.69%。（5）链霉菌NEAU6能够在生菜根际定殖,28天后在生菜根际土壤中数量为7.13×105CFU·g-1,根组织中数量为2.73×103CFU·g-1。（6）蔬菜品质指标的测定结果表明,当链霉菌NEAU6孢子量为6×107CFU·g-1时,生菜叶片中可溶性糖和可溶性蛋白含量分别提高了47.00%和20.53%;当链霉菌NEAU6孢子量为6×106CFU·g-1时,茼蒿、小白菜和香菜叶片中可溶性糖含量分别提高了79.65%、36.32%和46.45%。蔬菜叶绿素含量测定结果表明,链霉菌NEAU6对四种蔬菜叶片中叶绿素含量影响显著,生菜、茼蒿、小白菜和香菜叶片中叶绿素含量分别提高了51.89%、33.64%、52.14%和38.51%。（7）链霉菌NEAU6对蔬菜根际土壤中微生物种群数量的影响结果表明,经链霉菌NEAU6处理后,土壤中细菌数量增加了41.30%,放线菌数量增加了37.32%,真菌数量减少了24.64%。（8）通过对两株放线菌NEAU-85T和NEAU-YG30T进行多相分类学鉴定,确定NEAU-85T属于喜冷杆菌属,NEAU-YG30T属于野野村氏菌属。
Abstract
nong yao hua fei de guo du shi yong hui yin fa yan chong de nong ye sheng tai wu ran he shi pin an quan wen ti ,yi yi sheng jun wei he xin de wei sheng wu fei liao bu jin neng gou you xiao cu jin zuo wu sheng chang 、gai shan zuo wu pin zhi ,er ju bu hui dui tu rang sheng tai huan jing zao cheng wei xie 。xiang bi yu chuan tong ye ti fa jiao gong yi ,gu ti fa jiao gong yi ju you she bei jian chan 、cheng ben di 、wu ran shao deng you dian ,kuo yu zai nong ye ling yu ying yong 。yin ci ,tong guo gu ti fa jiao ji shu yan fa kuo yong yu nong ye sheng chan de sheng wu jun fei jiang wei nong ye sheng chan fa zhan dai lai xin ji yu 。ben yan jiu yi ju you cu sheng zuo yong de lian mei jun NEAU6wei yan jiu dui xiang ,tong guo gu ti fa jiao fang shi jin hang pei yang ,dui ji fa jiao ji zhi jin hang shai shua ,bing cai yong xiang ying mian fen xi fa dui gu ti fa jiao de pei yang tiao jian jin hang you hua 。li yong ping ban fa ya shi yan he pen zai shi yan tan jiu lian mei jun NEAU6dui sheng cai sheng chang de cu jin xiao guo ,tong guo tian jian shu cai chong zhi shi yan ,jin yi bu tan jiu le lian mei jun NEAU6de zui jia shi yong nong du ,ji ji dui shu cai pin zhi he xie lu su han liang de ying xiang ,bing yan jiu le jun zhu zai sheng cai gen ji de ding shi neng li ,zhi zai wei lian mei jun NEAU6sheng wu jun fei de gong ye hua sheng chan di gong li lun yi ju 。tong shi tan jiu le lian mei jun NEAU6dui tu rang wei sheng wu chong qun shu liang de ying xiang ,zai ci guo cheng zhong shai shua chu liang zhu fang xian jun xin chong ,bing jin hang le duo xiang fen lei xue jian ding 。ben yan jiu jie guo ru xia :（1）tong guo gu ti fa jiao ji zhi shai shua ,que ding lian mei jun NEAU6de zui jia gu ti fa jiao ji zhi wei qiu yin fen he mai fu ,bi li wei 3:1。cai yong xiang ying mian fen xi fa dui lian mei jun NEAU6de gu ti fa jiao pei yang tiao jian jin hang you hua ,zui jia fa jiao tiao jian wei jie chong liang 10%,han shui liang 70%,wen du 27°C,fa jiao 7tian shi ,lian mei jun NEAU6de bao zi liang da dao zui da ,wei 6.05×1011CFU·g-1。（2）sheng cai chong zi ping ban fa ya shi yan biao ming ,lian mei jun NEAU6neng gou xian zhe di gao sheng cai chong zi de ya chang he gen chang ,dang bao zi nong du wei 5.2×106CFU·mL-1shi ,ya chang di gao le 27.78%,gen chang di gao le 14.71%。（3）sheng cai pen zai shi yan biao ming ,dang lian mei jun NEAU6bao zi liang wei 6×107CFU·g-1shi ,sheng cai chan zhu chan liang di gao le 26.00%,zhu gao di gao le 12.35%,xie chang he xie kuan fen bie di gao le 8.00%he 15.60%。（4）tian jian shi yan biao ming ,dang lian mei jun NEAU6bao zi liang wei 6×107CFU·g-1shi ,xian zhe de cu jin le sheng cai he xiao bai cai de sheng chang ,yu dui zhao zu xiang bi ,chan zhu chan liang fen bie zeng jia le 48.00%he 37.06%。dang lian mei jun NEAU6bao zi liang wei 6×106CFU·g-1shi ,dui tong hao he xiang cai de cu sheng xiao guo xian zhe ,yu dui zhao zu xiang bi ,chan zhu chan liang fen bie zeng jia le 40.84%he 20.69%。（5）lian mei jun NEAU6neng gou zai sheng cai gen ji ding shi ,28tian hou zai sheng cai gen ji tu rang zhong shu liang wei 7.13×105CFU·g-1,gen zu zhi zhong shu liang wei 2.73×103CFU·g-1。（6）shu cai pin zhi zhi biao de ce ding jie guo biao ming ,dang lian mei jun NEAU6bao zi liang wei 6×107CFU·g-1shi ,sheng cai xie pian zhong ke rong xing tang he ke rong xing dan bai han liang fen bie di gao le 47.00%he 20.53%;dang lian mei jun NEAU6bao zi liang wei 6×106CFU·g-1shi ,tong hao 、xiao bai cai he xiang cai xie pian zhong ke rong xing tang han liang fen bie di gao le 79.65%、36.32%he 46.45%。shu cai xie lu su han liang ce ding jie guo biao ming ,lian mei jun NEAU6dui si chong shu cai xie pian zhong xie lu su han liang ying xiang xian zhe ,sheng cai 、tong hao 、xiao bai cai he xiang cai xie pian zhong xie lu su han liang fen bie di gao le 51.89%、33.64%、52.14%he 38.51%。（7）lian mei jun NEAU6dui shu cai gen ji tu rang zhong wei sheng wu chong qun shu liang de ying xiang jie guo biao ming ,jing lian mei jun NEAU6chu li hou ,tu rang zhong xi jun shu liang zeng jia le 41.30%,fang xian jun shu liang zeng jia le 37.32%,zhen jun shu liang jian shao le 24.64%。（8）tong guo dui liang zhu fang xian jun NEAU-85The NEAU-YG30Tjin hang duo xiang fen lei xue jian ding ,que ding NEAU-85Tshu yu xi leng gan jun shu ,NEAU-YG30Tshu yu ye ye cun shi jun shu 。
论文参考文献
论文详细介绍
论文作者分别是来自东北农业大学的王莹，发表于刊物东北农业大学2019-08-27论文，是一篇关于链霉菌论文,固体发酵论文,响应面分析论文,促生作用论文,东北农业大学2019-08-27论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自东北农业大学2019-08-27论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：链霉菌论文; 固体发酵论文; 响应面分析论文; 促生作用论文; 东北农业大学2019-08-27论文;