本文主要研究内容
作者（2019）在《母代效应影响飞蝗胚胎滞育调控的分子机制》一文中研究指出：飞蝗是全球范围的、最具破坏性的农业害虫。前人对它的生态学、生物学和防控方法有较全面的研究。如:飞蝗子代滞育受母代光周期影响。研究表明,FOXO在调控昆虫滞育中发挥重要作用。但光周期诱导母代飞蝗调控子代滞育的分子机制仍不清楚。本研究分别对飞蝗脂肪体、卵巢和中枢神经系统进行了转录组测序。其中长日照和短日照处理组对比,脂肪体和卵巢中有71个共有差异表达基因,中枢神经系统有84个差异表达基因涉及滞育诱导并且与FOXO信号途径密切相关,包括三羧酸循环、糖酵解,氧化磷酸化和PI3K-Akt途径等。随后,在所有处理组中随机选取36个差异基因进行qRT-PCR验证。结果显示差异基因的相对表达量与转录组数据一致。进一步选取rai1,shkC和takeout三个基因研究它们的滞育调控功能。我们分别克隆了这三个基因,合成了它们的双链RNA并注射羽化72小时内的飞蝗雌成虫。非滞育诱导条件的光周期为16 h:8h(L:D,温度28°C,相对湿度为60%。非滞育诱导条件的光周期为10 h:14 h(L:D),温度28°C,相对湿度为60%。结果表明,在长日照或短日照下与对照相比,干扰候选基因后,候选基因的相对表达量均显著降低。短日照下,dsrai1和dstakeout处理飞蝗卵滞育率显著高于对照蝗卵滞育率。dsshkC处理飞蝗卵滞育率显著低于对照蝗卵滞育率。另外,我们检测了dsrai1处理中SOD和ROS活性。结果表明,长日照和短日照下RNA干扰飞蝗rai1后,SOD和ROS活性均显著升高。本研究显示干扰母代飞蝗rai1和takeout能够抑制子代卵滞育,而干扰干扰母代飞蝗shkC能够促进子代卵滞育。另外,rai1负调控SOD和ROS。因此,我们推测光周期通过母代rai1和takeout基因影响FOXO信号途径,最终将环境信号传递至下一代蝗卵中诱导蝗卵滞育。本研究有助于揭示昆虫中母代光周期影响子代滞育的分子机制,对研究飞蝗种群爆发具有重要意义。
Abstract
fei huang shi quan qiu fan wei de 、zui ju po huai xing de nong ye hai chong 。qian ren dui ta de sheng tai xue 、sheng wu xue he fang kong fang fa you jiao quan mian de yan jiu 。ru :fei huang zi dai zhi yo shou mu dai guang zhou ji ying xiang 。yan jiu biao ming ,FOXOzai diao kong kun chong zhi yo zhong fa hui chong yao zuo yong 。dan guang zhou ji you dao mu dai fei huang diao kong zi dai zhi yo de fen zi ji zhi reng bu qing chu 。ben yan jiu fen bie dui fei huang zhi fang ti 、luan chao he zhong shu shen jing ji tong jin hang le zhuai lu zu ce xu 。ji zhong chang ri zhao he duan ri zhao chu li zu dui bi ,zhi fang ti he luan chao zhong you 71ge gong you cha yi biao da ji yin ,zhong shu shen jing ji tong you 84ge cha yi biao da ji yin she ji zhi yo you dao bing ju yu FOXOxin hao tu jing mi qie xiang guan ,bao gua san suo suan xun huan 、tang jiao jie ,yang hua lin suan hua he PI3K-Akttu jing deng 。sui hou ,zai suo you chu li zu zhong sui ji shua qu 36ge cha yi ji yin jin hang qRT-PCRyan zheng 。jie guo xian shi cha yi ji yin de xiang dui biao da liang yu zhuai lu zu shu ju yi zhi 。jin yi bu shua qu rai1,shkChe takeoutsan ge ji yin yan jiu ta men de zhi yo diao kong gong neng 。wo men fen bie ke long le zhe san ge ji yin ,ge cheng le ta men de shuang lian RNAbing zhu she yu hua 72xiao shi nei de fei huang ci cheng chong 。fei zhi yo you dao tiao jian de guang zhou ji wei 16 h:8h(L:D,wen du 28°C,xiang dui shi du wei 60%。fei zhi yo you dao tiao jian de guang zhou ji wei 10 h:14 h(L:D),wen du 28°C,xiang dui shi du wei 60%。jie guo biao ming ,zai chang ri zhao huo duan ri zhao xia yu dui zhao xiang bi ,gan rao hou shua ji yin hou ,hou shua ji yin de xiang dui biao da liang jun xian zhe jiang di 。duan ri zhao xia ,dsrai1he dstakeoutchu li fei huang luan zhi yo lv xian zhe gao yu dui zhao huang luan zhi yo lv 。dsshkCchu li fei huang luan zhi yo lv xian zhe di yu dui zhao huang luan zhi yo lv 。ling wai ,wo men jian ce le dsrai1chu li zhong SODhe ROShuo xing 。jie guo biao ming ,chang ri zhao he duan ri zhao xia RNAgan rao fei huang rai1hou ,SODhe ROShuo xing jun xian zhe sheng gao 。ben yan jiu xian shi gan rao mu dai fei huang rai1he takeoutneng gou yi zhi zi dai luan zhi yo ,er gan rao gan rao mu dai fei huang shkCneng gou cu jin zi dai luan zhi yo 。ling wai ,rai1fu diao kong SODhe ROS。yin ci ,wo men tui ce guang zhou ji tong guo mu dai rai1he takeoutji yin ying xiang FOXOxin hao tu jing ,zui zhong jiang huan jing xin hao chuan di zhi xia yi dai huang luan zhong you dao huang luan zhi yo 。ben yan jiu you zhu yu jie shi kun chong zhong mu dai guang zhou ji ying xiang zi dai zhi yo de fen zi ji zhi ,dui yan jiu fei huang chong qun bao fa ju you chong yao yi yi 。
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论文作者分别是来自中国农业科学院的，发表于刊物中国农业科学院2019-07-05论文，是一篇关于飞蝗论文,卵滞育论文,光周期论文,通路论文,实时荧光定量论文,中国农业科学院2019-07-05论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自中国农业科学院2019-07-05论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。