本文主要研究内容
作者何凌云（2019）在《大豆油或棕榈油替代鱼油对斜带石斑鱼生长性能、脂肪酸组成及脂肪代谢的影响》一文中研究指出：本试验以鱼粉、豆粕、酪蛋白和明胶为蛋白源,鱼油、豆油和棕榈油作为脂肪源,配制大豆油或棕榈油替代鱼油水平分别0、25%、50%、75%、100%9种等氮等脂试验饲料(对照组、25SO、50SO、75SO、100SO、25PO、50PO、75PO和100PO饲料组),将810尾初始均重约为12.59 g的斜带石斑鱼随机分到27个网箱中,每组3个重复,每个重复30尾鱼,每天早晚饲喂至表观饱食,试验周期为8周,比较探究大豆油、棕榈油替代鱼油对斜带石斑鱼生长性能、脂肪酸组成及脂肪代谢相关基因表达的影响及差异机制。主要研究结果如下:1.生长性能和体成分:无论大豆油还是棕榈油,随着替代鱼油水平增加,增重率(WGR)及肝脏与肌肉粗蛋白含量呈先升高后降低的趋势,但肝脏粗脂肪含量则呈逐渐升高趋势。5SO、100SO和100PO组肝脏粗脂肪含量显著高于对照组(P<0.05)。油源不影响斜带石斑鱼生长性能,但棕榈油组全鱼和肌肉粗蛋白含量高于大豆油组(P<0.05)。以增重率为测定指标,经过二次回归分析得到大豆油和棕榈油替代鱼油的最佳比例分别为32.9%和47.4%,以上结果表明,大豆油、棕榈油替代鱼油提高了肝脏粗脂肪含量,棕榈油组脂肪沉积高于大豆油组,棕榈油替代鱼油比例高于大豆油。2.血浆生化指标、肝脏抗氧化及脂肪代谢酶活:无论大豆油还是棕榈油,随替代鱼油水平增加,血浆T-CHO和HLD-C含量呈先升高后降低趋势,肝脏CAT活性和MDA含量呈逐渐降低趋势,而肝脏LPL和TL活性呈先降低后升高趋势。对照组和25SO组HLD-C、GSH-Px活性和MDA含量均显著高于100SO组(P<0.05),对照组HL活性显著低于100SO组(P<0.05),但50SO组中ACC和HSL活性显著低于100SO(P<0.05)。50PO组中血浆T-CHO和TG含量显著高于100PO组(P<0.05),50PO组TL活性显著低于75PO和100SO组(P<0.05),100PO组中血浆CAT活性和MDA含量显著低于对照组(P<0.05)。油源不影响肝脏代谢酶活性,但影响血浆TG含量和CAT活性和MDA含量。以上结果表明,25SO和50PO可相对提高机体脂肪代谢机能,100SO和100PO组的抗氧化能力和脂肪分解酶活性降低,脂肪酸合成酶活性升高。3.脂肪酸组成和肝脏组织形态:无论大豆油还是棕榈油,随替代鱼油水平的增加,肌肉和肝脏C18:1n-9和C18:2n-6含量呈逐渐升高的趋势,ARA、DHA、EPA含量呈逐渐降低的趋势。50SO-100SO组肌肉C18:1n-9含量显著低于对照组(P<0.05),50PO-100PO组肌肉ARA含量显著低于对照组(P<0.05);75PO和100PO组肝脏C18:1n-9含量显著高于对照组(P<0.05),50SO-100SO和100PO组肝脏C18:2n-6含量显著高于对照组(P<0.05),DHA均从50SO和50PO显著低于对照组(P<0.05)。油源明显影响肌肉中C18:1n-9、C18:2n-6、ARA、EPA、DHA含量以及肝脏C18:1n-9和C18:2n-6含量。肝脏组织结构中发现,与对照组比,100SO和100PO组出现细胞核偏移或消失,脂肪滴明显增加,细胞空泡化增加明显。以上结果表明,大豆油、棕榈油替代鱼油降低DHA和EPA含量,饲料脂肪酸含量对鱼体不同组织脂肪酸有不同的影响。4.肝脏代谢相关基因表达:随替代鱼油水平的增加,肝脏FAS、ACC、G6PD、PPARα、LPL、Δ6fad和SREBP-1c基因表达量均呈上升的趋势,但ATGL、FABP和HSL基因表达量呈降低的趋势,而CPT-1、Elovl5、Elovl4基因表达量呈现先升高后降低的趋势,SCD基因表达无变化。100SO组中FAS、ACC、G6PD和LPL基因表达量和100PO组FAS、ACC、G6PD、HSL、PPARα、LPL、Δ6fad和SREBP-1c基因表达量均显著高于对照组(P<0.05),但100PO和100SO组ATGL基因表达量和100PO组FABP和Elovl5基因表达量显著低于对照组(P<0.05),25SO组Elovl5和SREBP-1c基因表达量和25PO组Elovl4基因表达量显著高于对照组(P<0.05)。以上结果表明,100SO和100PO组上调了脂肪酸合成相关基因表达,下调了脂肪酸分解相关基因表达。综上,在本试验条件下,大豆油或棕榈油大比例甚至完全替代鱼油也不会对斜带石斑鱼生长产生明显影响,但降低了机体营养状况、抗氧化能力、脂肪分解酶活性和肝脏和肌肉DHA和EPA的含量,促进了肝脏脂肪沉积并造成肝脏组织结构损伤。
Abstract
ben shi yan yi yu fen 、dou po 、lao dan bai he ming jiao wei dan bai yuan ,yu you 、dou you he zong lv you zuo wei zhi fang yuan ,pei zhi da dou you huo zong lv you ti dai yu you shui ping fen bie 0、25%、50%、75%、100%9chong deng dan deng zhi shi yan si liao (dui zhao zu 、25SO、50SO、75SO、100SO、25PO、50PO、75POhe 100POsi liao zu ),jiang 810wei chu shi jun chong yao wei 12.59 gde xie dai dan ban yu sui ji fen dao 27ge wang xiang zhong ,mei zu 3ge chong fu ,mei ge chong fu 30wei yu ,mei tian zao wan si wei zhi biao guan bao shi ,shi yan zhou ji wei 8zhou ,bi jiao tan jiu da dou you 、zong lv you ti dai yu you dui xie dai dan ban yu sheng chang xing neng 、zhi fang suan zu cheng ji zhi fang dai xie xiang guan ji yin biao da de ying xiang ji cha yi ji zhi 。zhu yao yan jiu jie guo ru xia :1.sheng chang xing neng he ti cheng fen :mo lun da dou you hai shi zong lv you ,sui zhao ti dai yu you shui ping zeng jia ,zeng chong lv (WGR)ji gan zang yu ji rou cu dan bai han liang cheng xian sheng gao hou jiang di de qu shi ,dan gan zang cu zhi fang han liang ze cheng zhu jian sheng gao qu shi 。5SO、100SOhe 100POzu gan zang cu zhi fang han liang xian zhe gao yu dui zhao zu (P<0.05)。you yuan bu ying xiang xie dai dan ban yu sheng chang xing neng ,dan zong lv you zu quan yu he ji rou cu dan bai han liang gao yu da dou you zu (P<0.05)。yi zeng chong lv wei ce ding zhi biao ,jing guo er ci hui gui fen xi de dao da dou you he zong lv you ti dai yu you de zui jia bi li fen bie wei 32.9%he 47.4%,yi shang jie guo biao ming ,da dou you 、zong lv you ti dai yu you di gao le gan zang cu zhi fang han liang ,zong lv you zu zhi fang chen ji gao yu da dou you zu ,zong lv you ti dai yu you bi li gao yu da dou you 。2.xie jiang sheng hua zhi biao 、gan zang kang yang hua ji zhi fang dai xie mei huo :mo lun da dou you hai shi zong lv you ,sui ti dai yu you shui ping zeng jia ,xie jiang T-CHOhe HLD-Chan liang cheng xian sheng gao hou jiang di qu shi ,gan zang CAThuo xing he MDAhan liang cheng zhu jian jiang di qu shi ,er gan zang LPLhe TLhuo xing cheng xian jiang di hou sheng gao qu shi 。dui zhao zu he 25SOzu HLD-C、GSH-Pxhuo xing he MDAhan liang jun xian zhe gao yu 100SOzu (P<0.05),dui zhao zu HLhuo xing xian zhe di yu 100SOzu (P<0.05),dan 50SOzu zhong ACChe HSLhuo xing xian zhe di yu 100SO(P<0.05)。50POzu zhong xie jiang T-CHOhe TGhan liang xian zhe gao yu 100POzu (P<0.05),50POzu TLhuo xing xian zhe di yu 75POhe 100SOzu (P<0.05),100POzu zhong xie jiang CAThuo xing he MDAhan liang xian zhe di yu dui zhao zu (P<0.05)。you yuan bu ying xiang gan zang dai xie mei huo xing ,dan ying xiang xie jiang TGhan liang he CAThuo xing he MDAhan liang 。yi shang jie guo biao ming ,25SOhe 50POke xiang dui di gao ji ti zhi fang dai xie ji neng ,100SOhe 100POzu de kang yang hua neng li he zhi fang fen jie mei huo xing jiang di ,zhi fang suan ge cheng mei huo xing sheng gao 。3.zhi fang suan zu cheng he gan zang zu zhi xing tai :mo lun da dou you hai shi zong lv you ,sui ti dai yu you shui ping de zeng jia ,ji rou he gan zang C18:1n-9he C18:2n-6han liang cheng zhu jian sheng gao de qu shi ,ARA、DHA、EPAhan liang cheng zhu jian jiang di de qu shi 。50SO-100SOzu ji rou C18:1n-9han liang xian zhe di yu dui zhao zu (P<0.05),50PO-100POzu ji rou ARAhan liang xian zhe di yu dui zhao zu (P<0.05);75POhe 100POzu gan zang C18:1n-9han liang xian zhe gao yu dui zhao zu (P<0.05),50SO-100SOhe 100POzu gan zang C18:2n-6han liang xian zhe gao yu dui zhao zu (P<0.05),DHAjun cong 50SOhe 50POxian zhe di yu dui zhao zu (P<0.05)。you yuan ming xian ying xiang ji rou zhong C18:1n-9、C18:2n-6、ARA、EPA、DHAhan liang yi ji gan zang C18:1n-9he C18:2n-6han liang 。gan zang zu zhi jie gou zhong fa xian ,yu dui zhao zu bi ,100SOhe 100POzu chu xian xi bao he pian yi huo xiao shi ,zhi fang di ming xian zeng jia ,xi bao kong pao hua zeng jia ming xian 。yi shang jie guo biao ming ,da dou you 、zong lv you ti dai yu you jiang di DHAhe EPAhan liang ,si liao zhi fang suan han liang dui yu ti bu tong zu zhi zhi fang suan you bu tong de ying xiang 。4.gan zang dai xie xiang guan ji yin biao da :sui ti dai yu you shui ping de zeng jia ,gan zang FAS、ACC、G6PD、PPARα、LPL、Δ6fadhe SREBP-1cji yin biao da liang jun cheng shang sheng de qu shi ,dan ATGL、FABPhe HSLji yin biao da liang cheng jiang di de qu shi ,er CPT-1、Elovl5、Elovl4ji yin biao da liang cheng xian xian sheng gao hou jiang di de qu shi ,SCDji yin biao da mo bian hua 。100SOzu zhong FAS、ACC、G6PDhe LPLji yin biao da liang he 100POzu FAS、ACC、G6PD、HSL、PPARα、LPL、Δ6fadhe SREBP-1cji yin biao da liang jun xian zhe gao yu dui zhao zu (P<0.05),dan 100POhe 100SOzu ATGLji yin biao da liang he 100POzu FABPhe Elovl5ji yin biao da liang xian zhe di yu dui zhao zu (P<0.05),25SOzu Elovl5he SREBP-1cji yin biao da liang he 25POzu Elovl4ji yin biao da liang xian zhe gao yu dui zhao zu (P<0.05)。yi shang jie guo biao ming ,100SOhe 100POzu shang diao le zhi fang suan ge cheng xiang guan ji yin biao da ,xia diao le zhi fang suan fen jie xiang guan ji yin biao da 。zeng shang ,zai ben shi yan tiao jian xia ,da dou you huo zong lv you da bi li shen zhi wan quan ti dai yu you ye bu hui dui xie dai dan ban yu sheng chang chan sheng ming xian ying xiang ,dan jiang di le ji ti ying yang zhuang kuang 、kang yang hua neng li 、zhi fang fen jie mei huo xing he gan zang he ji rou DHAhe EPAde han liang ,cu jin le gan zang zhi fang chen ji bing zao cheng gan zang zu zhi jie gou sun shang 。
论文参考文献
论文详细介绍
论文作者分别是来自集美大学的何凌云，发表于刊物集美大学2019-07-12论文，是一篇关于斜带石斑鱼论文,大豆油论文,棕榈油论文,生长论文,脂肪酸论文,脂肪代谢论文,集美大学2019-07-12论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自集美大学2019-07-12论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：斜带石斑鱼论文; 大豆油论文; 棕榈油论文; 生长论文; 脂肪酸论文; 脂肪代谢论文; 集美大学2019-07-12论文;