本文主要研究内容
作者郭泽,李子绅,代晓燕,王英锋（2019）在《低钾胁迫下外源生长素对烟草根系生长及钾吸收的影响》一文中研究指出：【目的】探明生长素参与低钾胁迫下植株根系的生长发育及吸钾机制,同时为提高植物体内钾素水平提供理论依据。【方法】采用室内水培法,以模式植物烟草为试验材料,通过设置2个钾浓度(5、0.15 mmol/L)和5个外源生长素(3–吲哚乙酸)浓度(0、5、10、20、40μmol/L),对植物根系生理特征、内源生长素浓度、钾素累积及钾吸收动力学和相关钾离子通道基因转录表达进行比较研究。【结果】1)与正常钾水平相比,在低钾胁迫条件下,植株地上部干重显著降低15.6%;根系扫描8项指标中,除根平均直径外,其余7项指标值均显著降低;ATPase活性显著降低43.3%;主根尖、侧根尖及叶片内源生长素浓度显著升高;钾吸收动力学参数Vmax、Km值分别显著降低了89.2%、99.6%;植株根系、叶片钾浓度分别显著降低了93.0%、62.2%;根系中内流型钾离子通道基因Ntkc1的表达量显著降低56%。2)添加外源生长素后,正常供钾植株的根系干物质重、根系活力、主根尖及侧根尖内源生长素浓度有增加的趋势,Vmax值和内流型钾离子通道基因NKT2、NtKC1的表达量明显增加;低钾条件下,植株表现出和正常供钾相似的规律,除此之外,低钾植株的根系生长得到明显改善,ATPase活性和地上地下部钾素浓度明显增加,外流型钾离子通道基因Ntork1的表达量明显降低。3)当添加生长素浓度为10μmol/L时,与未添加生长素相比,正常供钾植株的地上地下部干重显著增加了6.05%、8.54%;根体积及根系交叠数显著增加16.5%、23.2%;根系活力显著增加了298%;Vmax值显著增加了118%;低钾植株地上地下部干重与不添加相比显著提高了5.61%、28.6%;根系活力达到113μg/(g·h), FW,为无添加生长素时的3.3倍;根系ATPase活性相对增加了87.5%;根系钾浓度显著增加250%;钾离子通道基因NKT2在根系中表达量显著增加了7.04倍,Ntork1在根系及叶片中表达量显著降低了49.5%、72.5%。【结论】低钾胁迫影响烟草根系生长及植株对钾素的吸收累积,添加适当浓度外源生长素可改善植株根系生长发育状况,增加内流型钾离子通道基因NKT2、NtKC1的表达量,降低外流型钾离子通道基因Ntork1的表达量,且提高植株钾吸收动力学参数Vmax值、降低Km值,从而提高了植株对钾离子的吸收能力与亲和力,进而增加植株钾素浓度。
Abstract
【mu de 】tan ming sheng chang su can yu di jia xie pai xia zhi zhu gen ji de sheng chang fa yo ji xi jia ji zhi ,tong shi wei di gao zhi wu ti nei jia su shui ping di gong li lun yi ju 。【fang fa 】cai yong shi nei shui pei fa ,yi mo shi zhi wu yan cao wei shi yan cai liao ,tong guo she zhi 2ge jia nong du (5、0.15 mmol/L)he 5ge wai yuan sheng chang su (3–yin duo yi suan )nong du (0、5、10、20、40μmol/L),dui zhi wu gen ji sheng li te zheng 、nei yuan sheng chang su nong du 、jia su lei ji ji jia xi shou dong li xue he xiang guan jia li zi tong dao ji yin zhuai lu biao da jin hang bi jiao yan jiu 。【jie guo 】1)yu zheng chang jia shui ping xiang bi ,zai di jia xie pai tiao jian xia ,zhi zhu de shang bu gan chong xian zhe jiang di 15.6%;gen ji sao miao 8xiang zhi biao zhong ,chu gen ping jun zhi jing wai ,ji yu 7xiang zhi biao zhi jun xian zhe jiang di ;ATPasehuo xing xian zhe jiang di 43.3%;zhu gen jian 、ce gen jian ji xie pian nei yuan sheng chang su nong du xian zhe sheng gao ;jia xi shou dong li xue can shu Vmax、Kmzhi fen bie xian zhe jiang di le 89.2%、99.6%;zhi zhu gen ji 、xie pian jia nong du fen bie xian zhe jiang di le 93.0%、62.2%;gen ji zhong nei liu xing jia li zi tong dao ji yin Ntkc1de biao da liang xian zhe jiang di 56%。2)tian jia wai yuan sheng chang su hou ,zheng chang gong jia zhi zhu de gen ji gan wu zhi chong 、gen ji huo li 、zhu gen jian ji ce gen jian nei yuan sheng chang su nong du you zeng jia de qu shi ,Vmaxzhi he nei liu xing jia li zi tong dao ji yin NKT2、NtKC1de biao da liang ming xian zeng jia ;di jia tiao jian xia ,zhi zhu biao xian chu he zheng chang gong jia xiang shi de gui lv ,chu ci zhi wai ,di jia zhi zhu de gen ji sheng chang de dao ming xian gai shan ,ATPasehuo xing he de shang de xia bu jia su nong du ming xian zeng jia ,wai liu xing jia li zi tong dao ji yin Ntork1de biao da liang ming xian jiang di 。3)dang tian jia sheng chang su nong du wei 10μmol/Lshi ,yu wei tian jia sheng chang su xiang bi ,zheng chang gong jia zhi zhu de de shang de xia bu gan chong xian zhe zeng jia le 6.05%、8.54%;gen ti ji ji gen ji jiao die shu xian zhe zeng jia 16.5%、23.2%;gen ji huo li xian zhe zeng jia le 298%;Vmaxzhi xian zhe zeng jia le 118%;di jia zhi zhu de shang de xia bu gan chong yu bu tian jia xiang bi xian zhe di gao le 5.61%、28.6%;gen ji huo li da dao 113μg/(g·h), FW,wei mo tian jia sheng chang su shi de 3.3bei ;gen ji ATPasehuo xing xiang dui zeng jia le 87.5%;gen ji jia nong du xian zhe zeng jia 250%;jia li zi tong dao ji yin NKT2zai gen ji zhong biao da liang xian zhe zeng jia le 7.04bei ,Ntork1zai gen ji ji xie pian zhong biao da liang xian zhe jiang di le 49.5%、72.5%。【jie lun 】di jia xie pai ying xiang yan cao gen ji sheng chang ji zhi zhu dui jia su de xi shou lei ji ,tian jia kuo dang nong du wai yuan sheng chang su ke gai shan zhi zhu gen ji sheng chang fa yo zhuang kuang ,zeng jia nei liu xing jia li zi tong dao ji yin NKT2、NtKC1de biao da liang ,jiang di wai liu xing jia li zi tong dao ji yin Ntork1de biao da liang ,ju di gao zhi zhu jia xi shou dong li xue can shu Vmaxzhi 、jiang di Kmzhi ,cong er di gao le zhi zhu dui jia li zi de xi shou neng li yu qin he li ,jin er zeng jia zhi zhu jia su nong du 。
论文参考文献
论文详细介绍
论文作者分别是来自植物营养与肥料学报的郭泽,李子绅,代晓燕,王英锋，发表于刊物植物营养与肥料学报2019年07期论文，是一篇关于烟草论文,根系论文,生长素论文,吲哚乙酸论文,低钾胁迫论文,钾素吸收论文,植物营养与肥料学报2019年07期论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自植物营养与肥料学报2019年07期论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：烟草论文; 根系论文; 生长素论文; 吲哚乙酸论文; 低钾胁迫论文; 钾素吸收论文; 植物营养与肥料学报2019年07期论文;