本文主要研究内容
作者张宇靖（2019）在《高纯竹叶碳苷黄酮纳米粒子及其护肤功效研究》一文中研究指出：本文以四个碳苷黄酮总量在90%以上的高纯竹叶碳苷黄酮制剂(H-BLF)为芯材,以芫根阴离子多糖(TP)为壁材,以ε-聚赖氨酸(ε-PL)为阳离子桥,采用离子交联法制成纳米混悬液,进而在γ-环糊精(γ-CD)的保护下、通过真空冷冻干燥得到高纯竹叶碳苷黄酮的纳米粒子(H-BLFnp),采用多种技术手段对其结构和性能进行表征;并分别采用体内外评价体系,对H-BLFnp的保湿、美白和延缓衰老的护肤功效进行研究。主要研究内容及结果汇总如下:(1)以高纯竹叶碳苷黄酮制剂(H-BLF;用HPLC法测得荭草苷、异荭草苷、牡荆苷、异牡荆苷四个竹叶特征性碳苷的总量合计≥90%)为芯材,采用离子交联法键合形成竹叶碳苷黄酮的纳米混悬液(H-BLFnd);采用正交试验得出其优化的制备工艺参数为:TP浓度3mg/mL,TP与H-BLF的质量比12:1(w/w),ε-PL浓度0.4 mg/mL,恒流泵流速300 mL/h。然后,在混悬液中添加8%(w/v)的Y-环糊精(γ-CD)作为冻干保护剂,经真空冷冻干燥得到高纯竹叶黄酮的纳米粒子(H-BLFnp)。H-BLFnp外观呈白色,粉体光洁平整,测得其包埋率为(92.39±0.34)%;复溶于水后平均粒径为(679.7±10.2)nm,分散系数(PDI)为0.349±0.004;表明H-BLFnp粒径分布均匀,分散度良好。(2)采用紫外光谱(UV)、红外光谱(FTIR)对H-BLFnp的结构进行表征,H-BLFnp的UV光谱呈现出典型的黄酮带I和带II吸收峰,FTIR图谱显示H-BLFnd已进入γ-CD的空腔中,形成了环糊精的包合物。采用差示扫描量热法(DSC)及热重分析法(TG)研究H-BLFnp的热稳定性,结果表明,相较于纳米混悬液(H-BLFnd),纳米粒子(H-BLFnp)的熔融温度提高了 7.91 ℃、热焓值增加了 38.36 J/g,分解温度从246℃提高至324℃;H-BLFnp表现了更高的热稳定性。(3)以甘油为阳性对照,在恒湿条件下测定H-BLFnp的保湿率;结果表明在第36h时H-BLFnp的保湿率显著高于甘油(p<0.05)。采用小鼠B16黑色素瘤细胞模型,以β-熊果苷为阳性对照,研究H-BLFnp对B16细胞体外培养过程中相关指标的影响;结果表明,H-BLFnp对B16细胞增殖、酪氨酸酶活性和黑色素生成均表现出浓度依赖的抑制作用,当H-BLFnp试样浓度为100 μg/mL时,抑制率分别为45.14%、72.55%和54.01%,均显著优于β-熊果苷(p<0.05)。小鼠皮肤刺激性和眼刺激性实验表明,H-BLFnp对皮肤及粘膜均无刺激作用,外用安全性高。(4)应用系列皮肤测试仪,对受试者使用含有5.0%H-BLFnp试样(人体涂抹量为2mg/cm2)前后的多项生理指标进行测定。结果表明,H-BLFnp能显著提高人体皮肤的水分含量、皮肤弹性、明亮度和光泽度,降低皮肤黑色素含量和经皮水分散失,表明H-BLFnp具有补水和减少水分散失、提高皮肤弹性、淡化色素和光泽皮肤的功效。综上所述,高纯竹叶碳苷黄酮纳米粒子(H-BLFnp)是一种组分天然、工艺绿色、亲水性好、性能稳定、安全无刺激的复合生物纳米材料,同时具有显著的保湿、美白和延缓衰老的生物学功效,可作为新型护肤功能因子,在日化产品(尤其是水润型产品)中具有良好的应用前景。
Abstract
ben wen yi si ge tan gan huang tong zong liang zai 90%yi shang de gao chun zhu xie tan gan huang tong zhi ji (H-BLF)wei xin cai ,yi yan gen yin li zi duo tang (TP)wei bi cai ,yi ε-ju lai an suan (ε-PL)wei yang li zi qiao ,cai yong li zi jiao lian fa zhi cheng na mi hun xuan ye ,jin er zai γ-huan hu jing (γ-CD)de bao hu xia 、tong guo zhen kong leng dong gan zao de dao gao chun zhu xie tan gan huang tong de na mi li zi (H-BLFnp),cai yong duo chong ji shu shou duan dui ji jie gou he xing neng jin hang biao zheng ;bing fen bie cai yong ti nei wai ping jia ti ji ,dui H-BLFnpde bao shi 、mei bai he yan huan cui lao de hu fu gong xiao jin hang yan jiu 。zhu yao yan jiu nei rong ji jie guo hui zong ru xia :(1)yi gao chun zhu xie tan gan huang tong zhi ji (H-BLF;yong HPLCfa ce de hong cao gan 、yi hong cao gan 、mu jing gan 、yi mu jing gan si ge zhu xie te zheng xing tan gan de zong liang ge ji ≥90%)wei xin cai ,cai yong li zi jiao lian fa jian ge xing cheng zhu xie tan gan huang tong de na mi hun xuan ye (H-BLFnd);cai yong zheng jiao shi yan de chu ji you hua de zhi bei gong yi can shu wei :TPnong du 3mg/mL,TPyu H-BLFde zhi liang bi 12:1(w/w),ε-PLnong du 0.4 mg/mL,heng liu beng liu su 300 mL/h。ran hou ,zai hun xuan ye zhong tian jia 8%(w/v)de Y-huan hu jing (γ-CD)zuo wei dong gan bao hu ji ,jing zhen kong leng dong gan zao de dao gao chun zhu xie huang tong de na mi li zi (H-BLFnp)。H-BLFnpwai guan cheng bai se ,fen ti guang jie ping zheng ,ce de ji bao mai lv wei (92.39±0.34)%;fu rong yu shui hou ping jun li jing wei (679.7±10.2)nm,fen san ji shu (PDI)wei 0.349±0.004;biao ming H-BLFnpli jing fen bu jun yun ,fen san du liang hao 。(2)cai yong zi wai guang pu (UV)、gong wai guang pu (FTIR)dui H-BLFnpde jie gou jin hang biao zheng ,H-BLFnpde UVguang pu cheng xian chu dian xing de huang tong dai Ihe dai IIxi shou feng ,FTIRtu pu xian shi H-BLFndyi jin ru γ-CDde kong qiang zhong ,xing cheng le huan hu jing de bao ge wu 。cai yong cha shi sao miao liang re fa (DSC)ji re chong fen xi fa (TG)yan jiu H-BLFnpde re wen ding xing ,jie guo biao ming ,xiang jiao yu na mi hun xuan ye (H-BLFnd),na mi li zi (H-BLFnp)de rong rong wen du di gao le 7.91 ℃、re han zhi zeng jia le 38.36 J/g,fen jie wen du cong 246℃di gao zhi 324℃;H-BLFnpbiao xian le geng gao de re wen ding xing 。(3)yi gan you wei yang xing dui zhao ,zai heng shi tiao jian xia ce ding H-BLFnpde bao shi lv ;jie guo biao ming zai di 36hshi H-BLFnpde bao shi lv xian zhe gao yu gan you (p<0.05)。cai yong xiao shu B16hei se su liu xi bao mo xing ,yi β-xiong guo gan wei yang xing dui zhao ,yan jiu H-BLFnpdui B16xi bao ti wai pei yang guo cheng zhong xiang guan zhi biao de ying xiang ;jie guo biao ming ,H-BLFnpdui B16xi bao zeng shi 、lao an suan mei huo xing he hei se su sheng cheng jun biao xian chu nong du yi lai de yi zhi zuo yong ,dang H-BLFnpshi yang nong du wei 100 μg/mLshi ,yi zhi lv fen bie wei 45.14%、72.55%he 54.01%,jun xian zhe you yu β-xiong guo gan (p<0.05)。xiao shu pi fu ci ji xing he yan ci ji xing shi yan biao ming ,H-BLFnpdui pi fu ji nian mo jun mo ci ji zuo yong ,wai yong an quan xing gao 。(4)ying yong ji lie pi fu ce shi yi ,dui shou shi zhe shi yong han you 5.0%H-BLFnpshi yang (ren ti tu ma liang wei 2mg/cm2)qian hou de duo xiang sheng li zhi biao jin hang ce ding 。jie guo biao ming ,H-BLFnpneng xian zhe di gao ren ti pi fu de shui fen han liang 、pi fu dan xing 、ming liang du he guang ze du ,jiang di pi fu hei se su han liang he jing pi shui fen san shi ,biao ming H-BLFnpju you bu shui he jian shao shui fen san shi 、di gao pi fu dan xing 、dan hua se su he guang ze pi fu de gong xiao 。zeng shang suo shu ,gao chun zhu xie tan gan huang tong na mi li zi (H-BLFnp)shi yi chong zu fen tian ran 、gong yi lu se 、qin shui xing hao 、xing neng wen ding 、an quan mo ci ji de fu ge sheng wu na mi cai liao ,tong shi ju you xian zhe de bao shi 、mei bai he yan huan cui lao de sheng wu xue gong xiao ,ke zuo wei xin xing hu fu gong neng yin zi ,zai ri hua chan pin (you ji shi shui run xing chan pin )zhong ju you liang hao de ying yong qian jing 。
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论文作者分别是来自浙江大学的张宇靖，发表于刊物浙江大学2019-04-12论文，是一篇关于高纯竹叶碳苷黄酮论文,化妆品功能因子论文,复合生物纳米粒子论文,护肤作用论文,浙江大学2019-04-12论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自浙江大学2019-04-12论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：高纯竹叶碳苷黄酮论文; 化妆品功能因子论文; 复合生物纳米粒子论文; 护肤作用论文; 浙江大学2019-04-12论文;