论文摘要
Smad蛋白是一种新发现的蛋白,它存在于人和动物多种细胞中,参与调节细胞增殖、分化、胚胎发育和骨骼重建,并在细胞外基质形成、创伤修复等生理病理过程中有重要作用,是TGF-β受体复合物的下游信号。它是目前所知的唯一的TGF-β R胞内激酶底物,Smad蛋白介导了TGF-β的胞内信号转导,能够与细胞膜上的TGF β受体（TGF β Preceptor,T β R）结合并将TGF β的信号传递至胞核内发挥生物效应。近年来随着对TGF-β/Smad信号传导系统研究的加深,TGF-β/Smad在器官纤维化中的作用逐渐引起了人们的重视。本实验应用四氯化碳诱发大鼠肝纤维化模型,在肝纤维化形成的不同时期采用RT-PCR和免疫组织化学方法检测肝组织中TGF-β/Smad信号传导通路中的Smad3,Smad7的表达变化,旨在进一步了解Smad3,Smad7在肝纤维化发生发展中的作用;另一部分研究内容为制备模型开始及4周起应用肝纤溶颗粒预防和治疗肝纤维化,并与正常对照组比较,观察各组肝纤维化血清学指标、肝功能情况、肝组织羟脯氨酸含量、病理纤维化程度分级及Smad3,Smad7,TGF-β 1基因及蛋白水平变化,旨在观察肝纤溶颗粒对TGF-β/Smad信号传导通路中的Smad3,Smad7及TGF-β 1表达的影响,并对其作用机制进行初步探讨,为临床防治肝纤维化提供依据。 本研究首次应用RT-PCR和免疫组化方法动态观察了TGF-β/Smad信号传导通路中Smad3,Smad7,TGF-β 1在肝纤维化形成过程中的表达变化,首次从细胞信号传导通路角度阐述肝纤溶颗粒抗肝纤维化的作用机制,提出了肝纤溶颗粒抗肝纤维化治疗的新靶点,为临床防治肝纤维化提供了一条新的途径和理论依据。
论文目录
相关论文文献
- [1].Regulatory Effect of Shenge Yifei Capsule on TGF-β1/Smad Signaling Pathway in Rats with Chronic Obstructive Pulmonary Disease[J]. Medicinal Plant 2020(01)
- [2].Linggui Zhugan Decoction(苓桂术甘汤) Inhibits Ventricular Remodeling after Acute Myocardial Infarction in Rats by Suppressing TGF-β1/Smad Signaling Pathway[J]. Chinese Journal of Integrative Medicine 2020(05)
- [3].TGFβ/Smad信号通路在口腔鳞癌热疗中的表达变化[J]. 实用口腔医学杂志 2019(01)
- [4].Wulong Xiaozheng Wan medicated serum inhibits epithelial-mesenchymal transition in human gastric carcinoma cell line BGC823 by modulation of transforming growth factor-β_1/Smad signaling[J]. Journal of Traditional Chinese Medicine 2019(03)
- [5].Involvement of Rho-associated coiled-coil kinase signaling inhibition in TGF-β1/Smad2,3 signal transduction in vitro[J]. International Journal of Ophthalmology 2017(12)
- [6].The Analysis of Relationship between MSTN/Smad Signal Path and Turpan Black Sheep Meat Production[J]. Agricultural Science & Technology 2016(01)
- [7].Association of Down-regulation of CD109 Expression with Up-expression of Smad7 in Pathogenesis of Psoriasis[J]. Journal of Huazhong University of Science and Technology(Medical Sciences) 2016(01)
- [8].脑星形细胞瘤组织TGF-β1、Smad7与VEGF的表达及意义[J]. 健康之路 2016(12)
- [9].Qingxuan Jiangya Decoction(清眩降压汤) Prevents Blood Pressure Elevation and Ameliorates Vascular Structural Remodeling via Modulating TGF-β 1/Smad Pathway in Spontaneously Hypertensive Rats[J]. Chinese Journal of Integrative Medicine 2020(03)
- [10].Early intervention with Di-Dang Decoction prevents macrovascular fibrosis in diabetic rats by regulating the TGF-β1/Smad signalling pathway[J]. Chinese Journal of Natural Medicines 2020(08)
- [11].Naringenin prevents experimental liver fibrosis by blocking TGFβ-Smad3 and JNK-Smad3 pathways[J]. World Journal of Gastroenterology 2017(24)
- [12].Anterior segment dysgenesis correlation with epithelial-mesenchymal transition in Smad4 knockout mice[J]. International Journal of Ophthalmology 2016(07)
- [13].Effect of bone marrow mesenchymal stem cells on the Smad expression of hepatic fibrosis rats[J]. Asian Pacific Journal of Tropical Medicine 2014(04)
- [14].Expression of USP15, TβR-Ⅰand Smad7 in Psoriasis[J]. Journal of Huazhong University of Science and Technology(Medical Sciences) 2014(03)
- [15].Smad蛋白研究进展[J]. 中国医学创新 2013(12)
- [16].Effect of the Protease Inhibitor MG132 on the Transforming Growth Factor-β/Smad Signaling Pathway in HSC-T6 Cells[J]. Journal of Huazhong University of Science and Technology(Medical Sciences) 2013(04)
- [17].Role of Smad7 in inflammatory bowel diseases[J]. World Journal of Gastroenterology 2012(40)
- [18].HSP70 decreases receptor-dependent phosphorylation of Smad2 and blocks TGF-β-induced epithelial-mesenchymal transition[J]. 遗传学报 2011(03)
- [19].Berbamine inhibits proliferation and induces apoptosis of KU812 cells by increasing Smad3 activity[J]. Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011(07)
- [20].Smad信号调控软骨形成机制的研究进展[J]. 国际口腔医学杂志 2010(05)
- [21].Smad7 dependent expression signature highlights BMP2 and HK2 signaling in HSC transdifferentiation[J]. World Journal of Gastroenterology 2010(41)
- [22].Smad蛋白在转化生长因子-β信号转导通路中的作用与机制[J]. 医学分子生物学杂志 2008(04)
- [23].微小RNA-21-5p靶向Smad7调控转化生长因子-β_1诱导心肌纤维化促进房颤的发生[J]. 临床内科杂志 2019(07)
- [24].孕期尼古丁暴露所致子代成骨细胞BMP-4/Smad信号通路的变化[J]. 医学研究杂志 2018(11)
- [25].白细胞介素37/Smad3在兔腹主动脉粥样硬化斑块中的表达研究[J]. 中国心血管杂志 2019(02)
- [26].三步序贯法对激素依赖型哮喘患者TGF-β_1/Smad信号通路及肺功能的影响[J]. 中华中医药杂志 2019(09)
- [27].牦牛bta-miR-1434-5p和Smad1基因组织表达谱及荧光素酶报告载体构建[J]. 中国农业大学学报 2018(05)
- [28].Effect of Banxia Xiexin decoction on Helicobacter pylori-related peptic ulcers and its possible mechanism via the TGF-β/Smad signaling pathway[J]. Journal of Traditional Chinese Medicine 2018(03)
- [29].San-Cao Granule (三草颗粒) Ameliorates Hepatic Fibrosis through High Mobility Group Box-1 Protein/Smad Signaling Pathway[J]. Chinese Journal of Integrative Medicine 2018(07)
- [30].Smad1基因在敖汉细毛羊发情不同时期卵巢中的表达规律研究[J]. 黑龙江畜牧兽医 2017(21)
标签：肝纤维化论文; 大鼠论文; 四氯化碳论文; 转化生长因子论文; 免疫组化论文; 预防与治疗论文; 肝纤溶颗粒论文;