本文主要研究内容
作者刘思巧,罗丹,李高钰,魏玉兰,马明东（2019）在《银杏毛状根褐化机制的初步研究》一文中研究指出：该实验依据现有发根农杆菌侵染法,以银杏无菌苗叶片为外植体,对农杆菌诱导银杏毛状根的实验操作进行优化,并观察记录银杏毛状根继代生长情况,测定继代不同天数的毛状根组织内多酚含量、多酚合成相关酶基因和POD基因相对表达量,以探讨银杏毛状根褐化的生理机制。结果显示:(1)银杏农杆菌侵染最适预培养时间为3d、菌种为R1601、共培养基为White、侵染时间为30min,优化侵染操作后的银杏毛状根诱导率可达72.36%;银杏毛状根继代生长的最适IBA浓度为0.5mg/L。(2)银杏毛状根初始发根时颜色为乳白色,多在伤口处簇生,伸长生长时无向地性,不分叶正背面都能在伤口处诱导出根,且在除菌培养20d时毛状根长可达0.4～0.8cm;继代培养25d时毛状根为白色,继代35d时部分毛状根从根部至中部开始转黄,继代45d时大部分毛状根由白转黄,根部至中部开始有褐化出现。(3)继代35d时毛状根的PAL、C4 H、4CL和POD基因表达量分别为25d的68%、44%、51%、52%;继代45d时PAL、C4 H、4CL、POD基因表达量分别为35d的19%、75%、86%、105%。(4)继代35d、45d的银杏毛状根总酚类含量分别为17.14和13.78mg/g。研究发现,PAL基因表达量变化与其下游的两个关键酶基因(C4 H、4CL)表达量变化不一致,且PAL基因表达量的大幅降低并未对下游C4 H、4CL基因表达有明显影响,说明PAL基因表达量变化对其下游合成速率影响不大,可能不是银杏毛状根苯丙氨酸代谢途径的限速酶;而C4 H与4CL基因表达量变化趋势高度一致,说明C4H应是该途径中的限速酶之一;推测继代前期可能是由PPO作为关键酶参与毛状根褐化反应,导致后期毛状根生长受抑制,POD基因表达水平随之上升,POD参与褐化反应。
Abstract
gai shi yan yi ju xian you fa gen nong gan jun qin ran fa ,yi yin xing mo jun miao xie pian wei wai zhi ti ,dui nong gan jun you dao yin xing mao zhuang gen de shi yan cao zuo jin hang you hua ,bing guan cha ji lu yin xing mao zhuang gen ji dai sheng chang qing kuang ,ce ding ji dai bu tong tian shu de mao zhuang gen zu zhi nei duo fen han liang 、duo fen ge cheng xiang guan mei ji yin he PODji yin xiang dui biao da liang ,yi tan tao yin xing mao zhuang gen he hua de sheng li ji zhi 。jie guo xian shi :(1)yin xing nong gan jun qin ran zui kuo yu pei yang shi jian wei 3d、jun chong wei R1601、gong pei yang ji wei White、qin ran shi jian wei 30min,you hua qin ran cao zuo hou de yin xing mao zhuang gen you dao lv ke da 72.36%;yin xing mao zhuang gen ji dai sheng chang de zui kuo IBAnong du wei 0.5mg/L。(2)yin xing mao zhuang gen chu shi fa gen shi yan se wei ru bai se ,duo zai shang kou chu cu sheng ,shen chang sheng chang shi mo xiang de xing ,bu fen xie zheng bei mian dou neng zai shang kou chu you dao chu gen ,ju zai chu jun pei yang 20dshi mao zhuang gen chang ke da 0.4～0.8cm;ji dai pei yang 25dshi mao zhuang gen wei bai se ,ji dai 35dshi bu fen mao zhuang gen cong gen bu zhi zhong bu kai shi zhuai huang ,ji dai 45dshi da bu fen mao zhuang gen you bai zhuai huang ,gen bu zhi zhong bu kai shi you he hua chu xian 。(3)ji dai 35dshi mao zhuang gen de PAL、C4 H、4CLhe PODji yin biao da liang fen bie wei 25dde 68%、44%、51%、52%;ji dai 45dshi PAL、C4 H、4CL、PODji yin biao da liang fen bie wei 35dde 19%、75%、86%、105%。(4)ji dai 35d、45dde yin xing mao zhuang gen zong fen lei han liang fen bie wei 17.14he 13.78mg/g。yan jiu fa xian ,PALji yin biao da liang bian hua yu ji xia you de liang ge guan jian mei ji yin (C4 H、4CL)biao da liang bian hua bu yi zhi ,ju PALji yin biao da liang de da fu jiang di bing wei dui xia you C4 H、4CLji yin biao da you ming xian ying xiang ,shui ming PALji yin biao da liang bian hua dui ji xia you ge cheng su lv ying xiang bu da ,ke neng bu shi yin xing mao zhuang gen ben bing an suan dai xie tu jing de xian su mei ;er C4 Hyu 4CLji yin biao da liang bian hua qu shi gao du yi zhi ,shui ming C4Hying shi gai tu jing zhong de xian su mei zhi yi ;tui ce ji dai qian ji ke neng shi you PPOzuo wei guan jian mei can yu mao zhuang gen he hua fan ying ,dao zhi hou ji mao zhuang gen sheng chang shou yi zhi ,PODji yin biao da shui ping sui zhi shang sheng ,PODcan yu he hua fan ying 。
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论文作者分别是来自西北植物学报的刘思巧,罗丹,李高钰,魏玉兰,马明东，发表于刊物西北植物学报2019年03期论文，是一篇关于毛状根论文,银杏论文,优化论文,酶促褐化论文,西北植物学报2019年03期论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自西北植物学报2019年03期论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：毛状根论文; 银杏论文; 优化论文; 酶促褐化论文; 西北植物学报2019年03期论文;