本文主要研究内容
作者张亮（2019）在《益生菌复合固体饮料对便秘的缓解作用及其机制研究》一文中研究指出：便秘是一种常见的肠道疾病,主要表现为排便困难、粪便干硬和每周排便次数小于3次,发病率呈现出逐年上升的趋势。短期便秘影响人们的正常工作和生活,长期便秘甚至会导致肾病、心血管疾病的发生,因此对便秘的预防和治疗应引起人们的重视。便秘的产生受多种因素的影响,包括生活和饮食习惯的改变、心理状态的变化等。临床上常使用大黄、泻剂等治疗便秘,长期使用会带来很大的依赖性。研究表明,便秘患者肠道内肠道菌群产生紊乱,双歧杆菌和乳杆菌减少,致病菌和条件致病菌增加。这提示我们可以通过体外补充益生菌来调节肠道菌群,从而恢复正常的肠道运动,达到缓解便秘的目的。因此,本研究使用含有已在便秘动物模型上验证有效的Bifidobacterium bifidum CCFM 16与其他功能菌株复配,并添加低聚果糖、低聚木糖、菊粉等益生元成分,得到新型商业化益生菌复合固体饮料,验证商业化益生菌复合固体饮料对小鼠便秘的缓解作用及其相关机制,并探究其对人群便秘的缓解能力和机制,分析动物数据和人群数据的对应性。首先建立便秘小鼠动物模型,使用盐酸洛哌丁胺诱导小鼠便秘,随后灌胃不同剂量的商业化益生菌复合固体饮料,测定便秘小鼠的排便状态(排便粒数和粪便含水量)、小肠推进率和首粒黑便时间,发现中、高剂量的益生菌溶液能显著提高便秘小鼠的排便粒数和粪便含水量,并缩短首粒黑便时间和提高小肠推进率,与CCFM 16缓解便秘的效果相当,且商业化益生菌复合固体饮料在整体上更趋向于恢复便秘症状至正常值。研究商业化益生菌复合固体饮料缓解便秘的机制,发现中、高益生菌剂量组中小鼠粪便的乙酸、丁酸水平显著升高,乙酸和丁酸对肠道蠕动具有促进作用,与CCFM 16提高乙酸和丙酸的实验结果有所差异。商业化益生菌复合固体饮料的摄入显著改变了便秘小鼠肠道中紊乱的菌群结构,提高了Firmicutes、Actinobacteria的水平,降低了Bacteroidetes、Proteobacteria的水平;在属水平上提高了Bifidobacterium、Lactobacillus、Clostridium、Ruminococcus和Coprococcus相对丰度,使Blautia的相对丰度降低,其中Clostridium、Ruminococcus和Coprococcus与SCFAs(short-chain fatty acids,SCFAs)的产生有关。商业化益生菌复合固体饮料也能够调节血清中相关胃肠调节肽的水平,使其恢复至正常值,但是对5-羟色胺(5-HT)的浓度没有影响。其次为了探究商业化益生菌复合固体饮料对人群便秘的缓解能力及其机制,本实验进行了一项针对便秘患者的产品试食反馈实验,结果显示商业化益生菌复合固体饮料、益生元和安慰剂(麦芽糊精)缓解便秘的总有效率分别为80.0%、41.0%和33.0%。与便秘动物模型相似,通过对人群粪便SCFAs的测定,发现益生菌复合固体饮料显著提高了乙酸的含量。商业化益生菌复合固体饮料对便秘患者的肠道菌群产生了复杂的影响,各组患者在服用商业化益生菌复合固体饮料前后其肠道菌群的多样性和门水平上的相对丰度无明显差异,在属水平上外源性益生菌Bifidobacterium和Lactobacillus呈现出上升的趋势,同时Parabacteroides和Coprococcus也有所提升,Clostridium的相对丰度显著上升且Blautia的相对丰度显著下降,这与便秘小鼠模型的结果部分吻合。总之,商业化益生菌复合固体饮料在合理的剂量内具有良好的缓解便秘能力,与CCFM 16相比更倾向于恢复小鼠的正常排便功能,其缓解便秘的机制可能与乙酸含量的提高有关。商业化益生菌复合固体饮料一方面通过益生菌的发酵作用直接提高乙酸含量,另一方面通过乳酸和氨基酸等其他代谢产物促进与产SCFAs相关的Clostridium和Coprococcus的生长繁殖,进而引起SCFAs浓度的变化,刺激肠道蠕动,从而恢复肠道的正常转运。
Abstract
bian bi shi yi chong chang jian de chang dao ji bing ,zhu yao biao xian wei pai bian kun nan 、fen bian gan ying he mei zhou pai bian ci shu xiao yu 3ci ,fa bing lv cheng xian chu zhu nian shang sheng de qu shi 。duan ji bian bi ying xiang ren men de zheng chang gong zuo he sheng huo ,chang ji bian bi shen zhi hui dao zhi shen bing 、xin xie guan ji bing de fa sheng ,yin ci dui bian bi de yu fang he zhi liao ying yin qi ren men de chong shi 。bian bi de chan sheng shou duo chong yin su de ying xiang ,bao gua sheng huo he yin shi xi guan de gai bian 、xin li zhuang tai de bian hua deng 。lin chuang shang chang shi yong da huang 、xie ji deng zhi liao bian bi ,chang ji shi yong hui dai lai hen da de yi lai xing 。yan jiu biao ming ,bian bi huan zhe chang dao nei chang dao jun qun chan sheng wen luan ,shuang qi gan jun he ru gan jun jian shao ,zhi bing jun he tiao jian zhi bing jun zeng jia 。zhe di shi wo men ke yi tong guo ti wai bu chong yi sheng jun lai diao jie chang dao jun qun ,cong er hui fu zheng chang de chang dao yun dong ,da dao huan jie bian bi de mu de 。yin ci ,ben yan jiu shi yong han you yi zai bian bi dong wu mo xing shang yan zheng you xiao de Bifidobacterium bifidum CCFM 16yu ji ta gong neng jun zhu fu pei ,bing tian jia di ju guo tang 、di ju mu tang 、ju fen deng yi sheng yuan cheng fen ,de dao xin xing shang ye hua yi sheng jun fu ge gu ti yin liao ,yan zheng shang ye hua yi sheng jun fu ge gu ti yin liao dui xiao shu bian bi de huan jie zuo yong ji ji xiang guan ji zhi ,bing tan jiu ji dui ren qun bian bi de huan jie neng li he ji zhi ,fen xi dong wu shu ju he ren qun shu ju de dui ying xing 。shou xian jian li bian bi xiao shu dong wu mo xing ,shi yong yan suan luo pai ding an you dao xiao shu bian bi ,sui hou guan wei bu tong ji liang de shang ye hua yi sheng jun fu ge gu ti yin liao ,ce ding bian bi xiao shu de pai bian zhuang tai (pai bian li shu he fen bian han shui liang )、xiao chang tui jin lv he shou li hei bian shi jian ,fa xian zhong 、gao ji liang de yi sheng jun rong ye neng xian zhe di gao bian bi xiao shu de pai bian li shu he fen bian han shui liang ,bing su duan shou li hei bian shi jian he di gao xiao chang tui jin lv ,yu CCFM 16huan jie bian bi de xiao guo xiang dang ,ju shang ye hua yi sheng jun fu ge gu ti yin liao zai zheng ti shang geng qu xiang yu hui fu bian bi zheng zhuang zhi zheng chang zhi 。yan jiu shang ye hua yi sheng jun fu ge gu ti yin liao huan jie bian bi de ji zhi ,fa xian zhong 、gao yi sheng jun ji liang zu zhong xiao shu fen bian de yi suan 、ding suan shui ping xian zhe sheng gao ,yi suan he ding suan dui chang dao ru dong ju you cu jin zuo yong ,yu CCFM 16di gao yi suan he bing suan de shi yan jie guo you suo cha yi 。shang ye hua yi sheng jun fu ge gu ti yin liao de she ru xian zhe gai bian le bian bi xiao shu chang dao zhong wen luan de jun qun jie gou ,di gao le Firmicutes、Actinobacteriade shui ping ,jiang di le Bacteroidetes、Proteobacteriade shui ping ;zai shu shui ping shang di gao le Bifidobacterium、Lactobacillus、Clostridium、Ruminococcushe Coprococcusxiang dui feng du ,shi Blautiade xiang dui feng du jiang di ,ji zhong Clostridium、Ruminococcushe Coprococcusyu SCFAs(short-chain fatty acids,SCFAs)de chan sheng you guan 。shang ye hua yi sheng jun fu ge gu ti yin liao ye neng gou diao jie xie qing zhong xiang guan wei chang diao jie tai de shui ping ,shi ji hui fu zhi zheng chang zhi ,dan shi dui 5-qiang se an (5-HT)de nong du mei you ying xiang 。ji ci wei le tan jiu shang ye hua yi sheng jun fu ge gu ti yin liao dui ren qun bian bi de huan jie neng li ji ji ji zhi ,ben shi yan jin hang le yi xiang zhen dui bian bi huan zhe de chan pin shi shi fan kui shi yan ,jie guo xian shi shang ye hua yi sheng jun fu ge gu ti yin liao 、yi sheng yuan he an wei ji (mai ya hu jing )huan jie bian bi de zong you xiao lv fen bie wei 80.0%、41.0%he 33.0%。yu bian bi dong wu mo xing xiang shi ,tong guo dui ren qun fen bian SCFAsde ce ding ,fa xian yi sheng jun fu ge gu ti yin liao xian zhe di gao le yi suan de han liang 。shang ye hua yi sheng jun fu ge gu ti yin liao dui bian bi huan zhe de chang dao jun qun chan sheng le fu za de ying xiang ,ge zu huan zhe zai fu yong shang ye hua yi sheng jun fu ge gu ti yin liao qian hou ji chang dao jun qun de duo yang xing he men shui ping shang de xiang dui feng du mo ming xian cha yi ,zai shu shui ping shang wai yuan xing yi sheng jun Bifidobacteriumhe Lactobacilluscheng xian chu shang sheng de qu shi ,tong shi Parabacteroideshe Coprococcusye you suo di sheng ,Clostridiumde xiang dui feng du xian zhe shang sheng ju Blautiade xiang dui feng du xian zhe xia jiang ,zhe yu bian bi xiao shu mo xing de jie guo bu fen wen ge 。zong zhi ,shang ye hua yi sheng jun fu ge gu ti yin liao zai ge li de ji liang nei ju you liang hao de huan jie bian bi neng li ,yu CCFM 16xiang bi geng qing xiang yu hui fu xiao shu de zheng chang pai bian gong neng ,ji huan jie bian bi de ji zhi ke neng yu yi suan han liang de di gao you guan 。shang ye hua yi sheng jun fu ge gu ti yin liao yi fang mian tong guo yi sheng jun de fa jiao zuo yong zhi jie di gao yi suan han liang ,ling yi fang mian tong guo ru suan he an ji suan deng ji ta dai xie chan wu cu jin yu chan SCFAsxiang guan de Clostridiumhe Coprococcusde sheng chang fan shi ,jin er yin qi SCFAsnong du de bian hua ,ci ji chang dao ru dong ,cong er hui fu chang dao de zheng chang zhuai yun 。
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论文作者分别是来自江南大学的张亮，发表于刊物江南大学2019-10-21论文，是一篇关于益生菌论文,便秘论文,肠道菌群论文,江南大学2019-10-21论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自江南大学2019-10-21论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：益生菌论文; 便秘论文; 肠道菌群论文; 江南大学2019-10-21论文;