本文主要研究内容
作者李洋（2019）在《亚急性瘤胃酸中毒对奶山羊瘤胃上皮挥发性脂肪酸吸收的影响及其机制研究》一文中研究指出：瘤胃发酵产生的挥发性脂肪酸(Volatile Fatty Acid,VFA)约50%～85%是直接被瘤胃上皮吸收、转运和代谢,对机体能量平衡调控起到了关键性作用。然而目前关于亚急性瘤胃酸中毒(Subacute Ruminal Acidosis,SARA)对瘤胃上皮VFA吸收的影响还尚不清楚。本研究以奶山羊为动物模型,采用瘤胃灌注技术和分子生物学技术研究SARA对瘤胃上皮乙酸、丙酸、丁酸的吸收率及其吸收代谢相关基因表达的影响,以期探索SARA对瘤胃上皮VFA吸收和代谢的影响机制。1.SARA对瘤胃上皮VFA吸收率的影响本试验采用完全随机设计,选取12只3岁左右、体重为62.13±4.76kg处于泌乳期的经产萨能奶山羊随机分成两组,即对照组(n=6)和SARA组(n=6)。对照组饲喂NFC/NDF=1.15日粮,SARA组依次饲喂NFC/NDF分别为1.15、1.49、2.12和2.66四种日粮。其中每种日粮饲喂15 d,以诱导奶山羊发生SARA。选取6只试验动物(对照组,n=3;SARA组,n=3)采用连续饲喂建立瘤胃相对稳态,通过乙酸、丙酸和丁酸启动-连续灌注,研究SARA对瘤胃上皮VFA产生速率、流通速率以及吸收速率的影响。结果显示:(1)与对照组相比,SARA组瘤胃pH值低于5.5持续时间达4.71 h/d。SARA组瘤胃液乙酸浓度和A/P显著下降(P<0.05),丙酸、丁酸和TVFA浓度显著升高(P<0.05);(2)SARA组乙酸产生速率和流通速率明显下降且吸收速率显著升高(P<0.05),丙酸和丁酸的产生速率、流通速率和吸收速率均明显升高(P<0.05),乙酸和丙酸的吸收率呈上升趋势(P>0.05),只有丁酸的吸收率显著提高(P<0.05)。这些结果表明:SARA改变瘤胃上皮对VFA产生速率、流通速率以及吸收率。2.SARA对瘤胃上皮VFA吸收代谢相关基因mRNA表达的影响试验动物及试验日粮同试验一。选取其余6只试验动物(对照组,n=3;SARA组,n=3)进行屠宰,采集瘤胃腹囊上皮组织采用实时定量PCR技术测定瘤胃上皮细胞VFA吸收和代谢相关基因mRNA表达量。结果显示:SARA组瘤胃上皮细胞VFA吸收相关基因MCT-1、NHE1、NHE3和DRA的mRNA表达量显著增加(P<0.05),瘤胃上皮细胞VFA代谢相关基因ACSS1和ACSS3的mRNA表达量显著降低(P<0.05),ACSS2、HMGCS1、HMGCS2 和 HMGCR 的 mRNA表达量则显著升高(P<0.05)。这些结果表明:SARA影响瘤胃上皮VFA吸收代谢相关基因mRNA表达。3.SARA对瘤胃上皮VFA吸收代谢相关基因蛋白表达量的影响试验动物、试验日粮及试验设计同试验二。采用Western blot技术检测瘤胃上皮细胞VFA吸收和代谢相关基因的蛋白表达量。结果显示:SARA组瘤胃上皮细胞VFA吸收基因NHE1、NHE3、NHE4、MCT-2、AE2和ATP1A1的蛋白表达量显著升高(P<0.05),同时VFA代谢基因ACSS2、ACSS3、HMGCL和ACAT1的蛋白表达量也显著升高(P<0.05)。这些结果表明:SARA影响瘤胃上皮VFA吸收代谢相关基因蛋白表达。上述结果表明:SARA使乙酸产生速率和流通速率明显下降而丙酸和丁酸反之,乙酸、丙酸和丁酸的吸收速率均明显升高,乙酸和丙酸的吸收率呈上升趋势,只有丁酸的吸收率显著提高,其机制与瘤胃pH值、瘤胃上皮细胞VFA吸收代谢相关基因mRNA以及蛋白表达最变化密切相关。
Abstract
liu wei fa jiao chan sheng de hui fa xing zhi fang suan (Volatile Fatty Acid,VFA)yao 50%～85%shi zhi jie bei liu wei shang pi xi shou 、zhuai yun he dai xie ,dui ji ti neng liang ping heng diao kong qi dao le guan jian xing zuo yong 。ran er mu qian guan yu ya ji xing liu wei suan zhong du (Subacute Ruminal Acidosis,SARA)dui liu wei shang pi VFAxi shou de ying xiang hai shang bu qing chu 。ben yan jiu yi nai shan yang wei dong wu mo xing ,cai yong liu wei guan zhu ji shu he fen zi sheng wu xue ji shu yan jiu SARAdui liu wei shang pi yi suan 、bing suan 、ding suan de xi shou lv ji ji xi shou dai xie xiang guan ji yin biao da de ying xiang ,yi ji tan suo SARAdui liu wei shang pi VFAxi shou he dai xie de ying xiang ji zhi 。1.SARAdui liu wei shang pi VFAxi shou lv de ying xiang ben shi yan cai yong wan quan sui ji she ji ,shua qu 12zhi 3sui zuo you 、ti chong wei 62.13±4.76kgchu yu bi ru ji de jing chan sa neng nai shan yang sui ji fen cheng liang zu ,ji dui zhao zu (n=6)he SARAzu (n=6)。dui zhao zu si wei NFC/NDF=1.15ri liang ,SARAzu yi ci si wei NFC/NDFfen bie wei 1.15、1.49、2.12he 2.66si chong ri liang 。ji zhong mei chong ri liang si wei 15 d,yi you dao nai shan yang fa sheng SARA。shua qu 6zhi shi yan dong wu (dui zhao zu ,n=3;SARAzu ,n=3)cai yong lian xu si wei jian li liu wei xiang dui wen tai ,tong guo yi suan 、bing suan he ding suan qi dong -lian xu guan zhu ,yan jiu SARAdui liu wei shang pi VFAchan sheng su lv 、liu tong su lv yi ji xi shou su lv de ying xiang 。jie guo xian shi :(1)yu dui zhao zu xiang bi ,SARAzu liu wei pHzhi di yu 5.5chi xu shi jian da 4.71 h/d。SARAzu liu wei ye yi suan nong du he A/Pxian zhe xia jiang (P<0.05),bing suan 、ding suan he TVFAnong du xian zhe sheng gao (P<0.05);(2)SARAzu yi suan chan sheng su lv he liu tong su lv ming xian xia jiang ju xi shou su lv xian zhe sheng gao (P<0.05),bing suan he ding suan de chan sheng su lv 、liu tong su lv he xi shou su lv jun ming xian sheng gao (P<0.05),yi suan he bing suan de xi shou lv cheng shang sheng qu shi (P>0.05),zhi you ding suan de xi shou lv xian zhe di gao (P<0.05)。zhe xie jie guo biao ming :SARAgai bian liu wei shang pi dui VFAchan sheng su lv 、liu tong su lv yi ji xi shou lv 。2.SARAdui liu wei shang pi VFAxi shou dai xie xiang guan ji yin mRNAbiao da de ying xiang shi yan dong wu ji shi yan ri liang tong shi yan yi 。shua qu ji yu 6zhi shi yan dong wu (dui zhao zu ,n=3;SARAzu ,n=3)jin hang tu zai ,cai ji liu wei fu nang shang pi zu zhi cai yong shi shi ding liang PCRji shu ce ding liu wei shang pi xi bao VFAxi shou he dai xie xiang guan ji yin mRNAbiao da liang 。jie guo xian shi :SARAzu liu wei shang pi xi bao VFAxi shou xiang guan ji yin MCT-1、NHE1、NHE3he DRAde mRNAbiao da liang xian zhe zeng jia (P<0.05),liu wei shang pi xi bao VFAdai xie xiang guan ji yin ACSS1he ACSS3de mRNAbiao da liang xian zhe jiang di (P<0.05),ACSS2、HMGCS1、HMGCS2 he HMGCR de mRNAbiao da liang ze xian zhe sheng gao (P<0.05)。zhe xie jie guo biao ming :SARAying xiang liu wei shang pi VFAxi shou dai xie xiang guan ji yin mRNAbiao da 。3.SARAdui liu wei shang pi VFAxi shou dai xie xiang guan ji yin dan bai biao da liang de ying xiang shi yan dong wu 、shi yan ri liang ji shi yan she ji tong shi yan er 。cai yong Western blotji shu jian ce liu wei shang pi xi bao VFAxi shou he dai xie xiang guan ji yin de dan bai biao da liang 。jie guo xian shi :SARAzu liu wei shang pi xi bao VFAxi shou ji yin NHE1、NHE3、NHE4、MCT-2、AE2he ATP1A1de dan bai biao da liang xian zhe sheng gao (P<0.05),tong shi VFAdai xie ji yin ACSS2、ACSS3、HMGCLhe ACAT1de dan bai biao da liang ye xian zhe sheng gao (P<0.05)。zhe xie jie guo biao ming :SARAying xiang liu wei shang pi VFAxi shou dai xie xiang guan ji yin dan bai biao da 。shang shu jie guo biao ming :SARAshi yi suan chan sheng su lv he liu tong su lv ming xian xia jiang er bing suan he ding suan fan zhi ,yi suan 、bing suan he ding suan de xi shou su lv jun ming xian sheng gao ,yi suan he bing suan de xi shou lv cheng shang sheng qu shi ,zhi you ding suan de xi shou lv xian zhe di gao ,ji ji zhi yu liu wei pHzhi 、liu wei shang pi xi bao VFAxi shou dai xie xiang guan ji yin mRNAyi ji dan bai biao da zui bian hua mi qie xiang guan 。
论文参考文献
论文详细介绍
论文作者分别是来自内蒙古农业大学的李洋，发表于刊物内蒙古农业大学2019-10-08论文，是一篇关于亚急性瘤胃酸中毒论文,挥发性脂肪酸论文,吸收率论文,吸收代谢相关基因论文,内蒙古农业大学2019-10-08论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自内蒙古农业大学2019-10-08论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：亚急性瘤胃酸中毒论文; 挥发性脂肪酸论文; 吸收率论文; 吸收代谢相关基因论文; 内蒙古农业大学2019-10-08论文;