本文主要研究内容
作者王思竹（2019）在《星星草PutACBP1基因的克隆及其功能分析》一文中研究指出：酰基辅酶A结合蛋白(Acyl-CoA-bindingproteins,ACBPs)是一类脂类载体蛋白,具有结合和运输脂类物质的功能,在植物生长发育和逆境(低温、氧化、干旱和重金属)应答调控过程中发挥了重要作用。但其在植物盐碱胁迫应答中的作用未见报道。星星草(Puccinellia tenuiflora 是一种单子叶禾本科盐生植物,是研究植物盐碱应答机制的理想模式植物。本研究,利用分子遗传学技术从星星草中克隆到PutACBP1基因,其开放阅读框为981 bp,编码326个氨基酸。PutACBPl是ACBP家族Class II亚族的成员之一,含有一个ACB结构域和Ankyrin结构域。荧光定量PCR分析表明,PutACBP1基因在星星草根、茎、叶、叶鞘、花、穗和种子均有表达,在叶中的表达量最高,在茎中表达量最低。在逆境(200 mmol/L NaCl、100 mmol/L NaHCO3、75 mmol/L Na2C03 胁迫、160 μmol/L CdCl2、10 mmol/L H2O2 和 4℃)胁迫时,根和叶中的PutACBP1都受诱导表达。这暗示着PutACBP1可能参与了盐碱、氧化、重金属和低温胁迫的应答过程。烟草(Nicotiana tabacum L.)叶片细胞和拟南芥(Arabidopsis thaliana)叶片细胞中定位分析表明,正常条件下PutACBP1定位于在细胞质。通过PutACBP1基因原核表达体系,诱导并纯化到(His)6-PutACBP1融合蛋白,利用磷脂结合实验初步证实了 PutACBP1具有与磷脂酰胆碱(Phosphatidylcholine,PC)结合的能力。通过构建pBI121-PutACBP1重组质粒,并利用农杆菌侵染法分别获得PutACBP1过表达拟南芥植株。对转PutACBP1基因拟南芥植株的耐盐性分析表明,在100 mmol/L和125 mmol/L NaCl胁迫下,过表达PutACBP1基因拟南芥的根长和鲜重都优于野生型拟南芥。拟南芥同源基因AtACBP2突变体植株(SALK-023077)的耐盐性分析表明,在100 mmol/L和125 mmol/L NaCl胁迫下,突变体拟南芥植株的根长和鲜重都低于野生型拟南芥。综上所述,在逆境应答过程中,PutACBP1对于植物盐胁迫应答具有正调控作用。同时PutACBP1可能通过与磷脂酰胆碱结合调控其运输到质膜,参与植物的脂类代谢。这些结果为深入研究星星草PuACBP1基因盐碱应答分子调控机理提供了新的证据。
Abstract
xian ji fu mei Ajie ge dan bai (Acyl-CoA-bindingproteins,ACBPs)shi yi lei zhi lei zai ti dan bai ,ju you jie ge he yun shu zhi lei wu zhi de gong neng ,zai zhi wu sheng chang fa yo he ni jing (di wen 、yang hua 、gan han he chong jin shu )ying da diao kong guo cheng zhong fa hui le chong yao zuo yong 。dan ji zai zhi wu yan jian xie pai ying da zhong de zuo yong wei jian bao dao 。xing xing cao (Puccinellia tenuiflora shi yi chong chan zi xie he ben ke yan sheng zhi wu ,shi yan jiu zhi wu yan jian ying da ji zhi de li xiang mo shi zhi wu 。ben yan jiu ,li yong fen zi wei chuan xue ji shu cong xing xing cao zhong ke long dao PutACBP1ji yin ,ji kai fang yue dou kuang wei 981 bp,bian ma 326ge an ji suan 。PutACBPlshi ACBPjia zu Class IIya zu de cheng yuan zhi yi ,han you yi ge ACBjie gou yu he Ankyrinjie gou yu 。ying guang ding liang PCRfen xi biao ming ,PutACBP1ji yin zai xing xing cao gen 、jing 、xie 、xie qiao 、hua 、sui he chong zi jun you biao da ,zai xie zhong de biao da liang zui gao ,zai jing zhong biao da liang zui di 。zai ni jing (200 mmol/L NaCl、100 mmol/L NaHCO3、75 mmol/L Na2C03 xie pai 、160 μmol/L CdCl2、10 mmol/L H2O2 he 4℃)xie pai shi ,gen he xie zhong de PutACBP1dou shou you dao biao da 。zhe an shi zhao PutACBP1ke neng can yu le yan jian 、yang hua 、chong jin shu he di wen xie pai de ying da guo cheng 。yan cao (Nicotiana tabacum L.)xie pian xi bao he ni na gai (Arabidopsis thaliana)xie pian xi bao zhong ding wei fen xi biao ming ,zheng chang tiao jian xia PutACBP1ding wei yu zai xi bao zhi 。tong guo PutACBP1ji yin yuan he biao da ti ji ,you dao bing chun hua dao (His)6-PutACBP1rong ge dan bai ,li yong lin zhi jie ge shi yan chu bu zheng shi le PutACBP1ju you yu lin zhi xian dan jian (Phosphatidylcholine,PC)jie ge de neng li 。tong guo gou jian pBI121-PutACBP1chong zu zhi li ,bing li yong nong gan jun qin ran fa fen bie huo de PutACBP1guo biao da ni na gai zhi zhu 。dui zhuai PutACBP1ji yin ni na gai zhi zhu de nai yan xing fen xi biao ming ,zai 100 mmol/Lhe 125 mmol/L NaClxie pai xia ,guo biao da PutACBP1ji yin ni na gai de gen chang he xian chong dou you yu ye sheng xing ni na gai 。ni na gai tong yuan ji yin AtACBP2tu bian ti zhi zhu (SALK-023077)de nai yan xing fen xi biao ming ,zai 100 mmol/Lhe 125 mmol/L NaClxie pai xia ,tu bian ti ni na gai zhi zhu de gen chang he xian chong dou di yu ye sheng xing ni na gai 。zeng shang suo shu ,zai ni jing ying da guo cheng zhong ,PutACBP1dui yu zhi wu yan xie pai ying da ju you zheng diao kong zuo yong 。tong shi PutACBP1ke neng tong guo yu lin zhi xian dan jian jie ge diao kong ji yun shu dao zhi mo ,can yu zhi wu de zhi lei dai xie 。zhe xie jie guo wei shen ru yan jiu xing xing cao PuACBP1ji yin yan jian ying da fen zi diao kong ji li di gong le xin de zheng ju 。
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论文作者分别是来自东北林业大学的王思竹，发表于刊物东北林业大学2019-11-18论文，是一篇关于星星草论文,酰基辅酶结合蛋白论文,胁迫论文,功能论文,东北林业大学2019-11-18论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自东北林业大学2019-11-18论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：星星草论文; 酰基辅酶结合蛋白论文; 胁迫论文; 功能论文; 东北林业大学2019-11-18论文;