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作者（2019）在《Effects of Macronutrients on Primary and Secondary Metabolism in Scutellaria baicalensis Georgi》一文中研究指出：[Objectives] This study was conducted to clarify the roles of macronutrients in regulating plant primary and secondary metabolism of Scutellaria baicalensis Georgi. [Methods] The contents of chlorophyll（Chl） and soluble sugar were detected with ultraviolet spectrophotometry. The activities of phenylalanine ammonia-lyase（PAL）, cinnamate 4-hydroxylase（C4H） and chalcone synthase（CHS） were determined with ultraviolet spectrophotometry. Secondary metabolites were detected by high performance liquid chromatography（HPLC）. [Results] The content of chlorophyll in treatments N, P and K was increased, showing significant difference from that of the control（P<0.05）. Among them, Treatment N has the highest content of chlorophyll. Soluble sugar content in treatments N, P and K（K>N>P>CK） was increased considerably and had significant difference from that of the control（P<0.05）. Compared with the control, PAL and C4H activity in treatments N, P and K were increased dramatically by 186.57%, 134.09%, 306.91% and 73.21%, 28.91%, 247.57%, respectively. CHS activity was also increased sharply in treatments N, P and K. Scutellarin, baicalin, wogonoside, baicalein, wogonin and oroxylin A contents in all treatments N, P and K（K>N>P） were increased, and had significant difference from that of the control（P<0.05）. [Conclusions] The results showed that macronutrients can increase leaf chlorophyll content, soluble sugar content, PAL activity, C4H activity and secondary metabolites of S. baicalensis.
Abstract
[Objectives] This study was conducted to clarify the roles of macronutrients in regulating plant primary and secondary metabolism of Scutellaria baicalensis Georgi. [Methods] The contents of chlorophyll（Chl） and soluble sugar were detected with ultraviolet spectrophotometry. The activities of phenylalanine ammonia-lyase（PAL）, cinnamate 4-hydroxylase（C4H） and chalcone synthase（CHS） were determined with ultraviolet spectrophotometry. Secondary metabolites were detected by high performance liquid chromatography（HPLC）. [Results] The content of chlorophyll in treatments N, P and K was increased, showing significant difference from that of the control（P<0.05）. Among them, Treatment N has the highest content of chlorophyll. Soluble sugar content in treatments N, P and K（K>N>P>CK） was increased considerably and had significant difference from that of the control（P<0.05）. Compared with the control, PAL and C4H activity in treatments N, P and K were increased dramatically by 186.57%, 134.09%, 306.91% and 73.21%, 28.91%, 247.57%, respectively. CHS activity was also increased sharply in treatments N, P and K. Scutellarin, baicalin, wogonoside, baicalein, wogonin and oroxylin A contents in all treatments N, P and K（K>N>P） were increased, and had significant difference from that of the control（P<0.05）. [Conclusions] The results showed that macronutrients can increase leaf chlorophyll content, soluble sugar content, PAL activity, C4H activity and secondary metabolites of S. baicalensis.
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