本文主要研究内容
作者邓婷（2019）在《邻苯二甲酸二丁酯对小鼠2型糖尿病的影响及分子机制探究》一文中研究指出：糖尿病的发病率在世界范围内不断增加,已成为危害人类健康的主要疾病之一,邻苯二甲酸酯被认为与糖尿病的发生发展密切相关。流行病学研究表明某些邻苯二甲酸酯暴露与糖尿病之间存在密切联系。然而,关于邻苯二甲酸二丁酯(Dibutyl phthalate,DBP)暴露对糖尿病发展的影响和作用机制还知之甚少。为了确定DBP暴露对2型糖尿病发生发展的作用,通过给予小鼠高脂高糖饮食和注射40 mg/kg低剂量链脲酶素(Streptozotocin,STZ)建立2型糖尿病(Type 2 diabetes mellitus,T2DM)小鼠模型。同时对小鼠进行剂量分别为0.5,5,50 mg/kg/day DBP经口暴露7周。实验结果显示单独暴露于50 mg/kg/day DBP能够导致胰岛素分泌和葡萄糖耐量的显著降低,但对胰岛素抵抗没有影响。而DBP结合高脂高糖饮食和STZ建模处理的实验结果显示,DBP暴露显著加重葡萄糖耐受不良,胰岛素耐受和胰岛素抵抗,并诱导胰腺和肾脏病变。胰岛素信号及其响应机制与T2DM的发生密切相关。本研究发现DBP暴露可破坏磷脂酰肌醇-3-激酶(Phosphatidylinositol-3-kinase,PI3K)表达和AKT磷酸化,并降低胰腺中葡萄糖转运蛋白-2(Glucose transporter-2,GLUT-2)的表达水平。给予脱甲基苯醌-B1(Demethylbenzoquinone-Bl,DMAQ-B1)处理,则显著提高了PI3K,AKT磷酸化和GLUT-2表达水平,能够有效抑制糖尿病的加重。研究结果表明,DBP通过破坏胰岛素信号通路和损害胰岛素分泌而加重T2DM。实验数据还显示DBP暴露诱导肝肾ROS水平增加,以及胰腺抗氧化因子超氧化物歧化酶(Superoxide dismutase,SOD)表达减少,并伴随着胰岛素分泌的减少和空腹血糖水平和胰岛素抵抗水平的增加。而使用抗氧化剂维生素E(Vitamin E,VE)以阻断氧化应激,可缓解这些症状,DBP暴露引起的氧化损伤可能是糖尿病病变的原因之一。本研究结果表明DBP通过氧化损伤导致葡萄糖运输和胰岛细胞的破坏,胰岛细胞凋亡促进2型糖尿病的发展。此外,DBP暴露导致了低密度脂蛋白(Low density lipoprotein,LDL-C),高密度脂蛋白(High density lipoprotein,HDL-C)、总胆固醇(Total cholesterol,TC)的增加以及游离脂肪酸(Free fatty acids,FFA)的降低,这表明DBP可能影响脂质代谢。综上,本实验的研究结果表明,DBP可以通过干扰胰岛素信号,导致氧化损伤及脂代谢异常等机制促进T2DM的发展。
Abstract
tang niao bing de fa bing lv zai shi jie fan wei nei bu duan zeng jia ,yi cheng wei wei hai ren lei jian kang de zhu yao ji bing zhi yi ,lin ben er jia suan zhi bei ren wei yu tang niao bing de fa sheng fa zhan mi qie xiang guan 。liu hang bing xue yan jiu biao ming mou xie lin ben er jia suan zhi bao lou yu tang niao bing zhi jian cun zai mi qie lian ji 。ran er ,guan yu lin ben er jia suan er ding zhi (Dibutyl phthalate,DBP)bao lou dui tang niao bing fa zhan de ying xiang he zuo yong ji zhi hai zhi zhi shen shao 。wei le que ding DBPbao lou dui 2xing tang niao bing fa sheng fa zhan de zuo yong ,tong guo gei yu xiao shu gao zhi gao tang yin shi he zhu she 40 mg/kgdi ji liang lian niao mei su (Streptozotocin,STZ)jian li 2xing tang niao bing (Type 2 diabetes mellitus,T2DM)xiao shu mo xing 。tong shi dui xiao shu jin hang ji liang fen bie wei 0.5,5,50 mg/kg/day DBPjing kou bao lou 7zhou 。shi yan jie guo xian shi chan du bao lou yu 50 mg/kg/day DBPneng gou dao zhi yi dao su fen bi he pu tao tang nai liang de xian zhe jiang di ,dan dui yi dao su di kang mei you ying xiang 。er DBPjie ge gao zhi gao tang yin shi he STZjian mo chu li de shi yan jie guo xian shi ,DBPbao lou xian zhe jia chong pu tao tang nai shou bu liang ,yi dao su nai shou he yi dao su di kang ,bing you dao yi xian he shen zang bing bian 。yi dao su xin hao ji ji xiang ying ji zhi yu T2DMde fa sheng mi qie xiang guan 。ben yan jiu fa xian DBPbao lou ke po huai lin zhi xian ji chun -3-ji mei (Phosphatidylinositol-3-kinase,PI3K)biao da he AKTlin suan hua ,bing jiang di yi xian zhong pu tao tang zhuai yun dan bai -2(Glucose transporter-2,GLUT-2)de biao da shui ping 。gei yu tuo jia ji ben kun -B1(Demethylbenzoquinone-Bl,DMAQ-B1)chu li ,ze xian zhe di gao le PI3K,AKTlin suan hua he GLUT-2biao da shui ping ,neng gou you xiao yi zhi tang niao bing de jia chong 。yan jiu jie guo biao ming ,DBPtong guo po huai yi dao su xin hao tong lu he sun hai yi dao su fen bi er jia chong T2DM。shi yan shu ju hai xian shi DBPbao lou you dao gan shen ROSshui ping zeng jia ,yi ji yi xian kang yang hua yin zi chao yang hua wu qi hua mei (Superoxide dismutase,SOD)biao da jian shao ,bing ban sui zhao yi dao su fen bi de jian shao he kong fu xie tang shui ping he yi dao su di kang shui ping de zeng jia 。er shi yong kang yang hua ji wei sheng su E(Vitamin E,VE)yi zu duan yang hua ying ji ,ke huan jie zhe xie zheng zhuang ,DBPbao lou yin qi de yang hua sun shang ke neng shi tang niao bing bing bian de yuan yin zhi yi 。ben yan jiu jie guo biao ming DBPtong guo yang hua sun shang dao zhi pu tao tang yun shu he yi dao xi bao de po huai ,yi dao xi bao diao wang cu jin 2xing tang niao bing de fa zhan 。ci wai ,DBPbao lou dao zhi le di mi du zhi dan bai (Low density lipoprotein,LDL-C),gao mi du zhi dan bai (High density lipoprotein,HDL-C)、zong dan gu chun (Total cholesterol,TC)de zeng jia yi ji you li zhi fang suan (Free fatty acids,FFA)de jiang di ,zhe biao ming DBPke neng ying xiang zhi zhi dai xie 。zeng shang ,ben shi yan de yan jiu jie guo biao ming ,DBPke yi tong guo gan rao yi dao su xin hao ,dao zhi yang hua sun shang ji zhi dai xie yi chang deng ji zhi cu jin T2DMde fa zhan 。
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论文详细介绍
论文作者分别是来自华中师范大学的邓婷，发表于刊物华中师范大学2019-09-29论文，是一篇关于邻苯二甲酸二丁酯论文,型糖尿病论文,胰岛素抵抗论文,胰岛素信号通路论文,氧化应激论文,华中师范大学2019-09-29论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自华中师范大学2019-09-29论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：邻苯二甲酸二丁酯论文; 型糖尿病论文; 胰岛素抵抗论文; 胰岛素信号通路论文; 氧化应激论文; 华中师范大学2019-09-29论文;