本文主要研究内容
作者（2019）在《Rapid Creation of New Photoperiod-/Thermo-Sensitive Genic Male-Sterile Rice Materials by CRISPR/Cas9 System》一文中研究指出：Rice is an important food crop in China, and the development of hybrid rice is a crucial way to increase grain yield. The creation of dual-purpose nuclear-sterile lines for two-line hybrid breeding has become vital for commercial rice breeding. We constructed the pC1300-2 x35 S::Cas9-sgRNAPTGMS2-1 expression vector for editing the male fertility gene PTGMS2-1 in two widely compatible rice varieties, 93-11 and Huazhan, by using the CRISPR/Cas9 system. We obtained the marker-free photoperiod-/thermo-sensitive genic male-sterile（P/TGMS） lines in T1 generation. According to the experiments in phytotron with four temperature and photoperiod treatments, we found the temperature is the main factor for restoring the pollen fertility of ptgms2-1 mutants in 93-11 and Huazhan, and the photoperiod also has some effects on pollen fertility in two different rice backgrounds. The application of cultivating new male-sterile lines by genome editing system will significantly accelerate the rice breeding process.
Abstract
Rice is an important food crop in China, and the development of hybrid rice is a crucial way to increase grain yield. The creation of dual-purpose nuclear-sterile lines for two-line hybrid breeding has become vital for commercial rice breeding. We constructed the pC1300-2 x35 S::Cas9-sgRNAPTGMS2-1 expression vector for editing the male fertility gene PTGMS2-1 in two widely compatible rice varieties, 93-11 and Huazhan, by using the CRISPR/Cas9 system. We obtained the marker-free photoperiod-/thermo-sensitive genic male-sterile（P/TGMS） lines in T1 generation. According to the experiments in phytotron with four temperature and photoperiod treatments, we found the temperature is the main factor for restoring the pollen fertility of ptgms2-1 mutants in 93-11 and Huazhan, and the photoperiod also has some effects on pollen fertility in two different rice backgrounds. The application of cultivating new male-sterile lines by genome editing system will significantly accelerate the rice breeding process.
论文参考文献
[1].Isolation of cDNA fragment of fertility-related gene inphotoperiod-sensitive genic male sterile rice[J]. KANG Jian, CHEN Fan and WU Naihu * 1. Institute of Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China. * Corresponding author. Chinese Science Bulletin.1998(24)[2].Inducing fertility restoration in the genic male sterile line of rice with chemical regulators[J]. WANG Kaizhi,CNRRI. Chinese Rice Research Newsletter.1994(03)[3].基于体细胞组织培养的甘蓝型油菜核三系保持系绵7MB-1亲本繁殖与F1制种（英文）[J]. 蒙大庆,袁代斌,张跃非,郭子荣,胥岚,李芝凡,蒲定福,汤天泽,贺启川,陈军. Agricultural Science & Technology.2009(01)[4].Studies on the Photoperiod Sensitive Characters of Male Fertility Alteration of Peiai64S’Main Male Genie Sterile Gene[J]. ZENG Han-lai,ZHANG Duan-pin,ZHANG Zhi-yu,YI Wen-kai,ZHU Xin and MENG Hui-jun(National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University , Wuhan 430070 , P. R . China ). Agricultural Sciences in China.2002(05)[5].温光条件对胞质不育系V太911289a雄性育性影响的初步研究（英文）[J]. 王丰青,巍荷,何觉民,何仪. Agricultural Science & Technology.2008(02)[6].Plant Temperature for Sterile Alteration of a Temperature-Sensitive Genic Male Sterile Rice,Peiai64S[J]. L Chuan-gen1,ZOU Jiang-shi1,HU Ning2 and YAO Ke-min2 1 Jiangsu Academy of Agricultural Sciences,Nanjing 210014,P.R.China 2 Nanjing University of Information Science and Technology,Nanjing 210044,P.R.China. Agricultural Sciences in China.2007(11)[7].Effect of day length and temperature on the pollen fertility in photo(thermo)-sensitive genic male-sterile rice[J]. HUANG Qinglui and TANG Xihua,Shanghai Inst of Piant physiology,Acad Sinica,Xhanghai 200032,China. Chinese Rice Research Newsletter.1996(02)[8].Effect of day length and temperature on the pollen fertility in photo (thermo)-sensitive genic male-sterile rice[J]. HUANG Qingliu and TANG Xihua,Shanghai Inst of Plant physiology,Acad Sinica,Shanghai 200032,China. Chinese Rice Research Newsletter.1996(02)[9].Breeding of Maintainer Line and Sterile Line Brown Planthopper Resistant Rice Using Marker-assisted Selection[J]. Hui GUO,Rui FENG,Can CHEN,Xiaoli ZHANG,Bailong LIU,Xueyi QIN. Asian Agricultural Research.2017(07)[10].The effects of dark-broken by UV-B,UV-A,and blue light on fertility of PGMR Nongken 58s[J]. YUE Bing LI Hesheng FENG Shengqiu Agro Dept.Huazhong Agri Univ,Wuhan 430070,China. Chinese Rice Research Newsletter.1998(02)
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：Rapid Creation of New Photoperiod-/Thermo-Sensitive Genic Male-Sterile Rice Materials by CRISPR/Cas9 System论文
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