论文摘要
龙须菜(Gracilaria lemaneiformis)已经成为我国继海带和紫菜之后的第三大栽培海藻,不仅是江蓠属的一个重要的产琼胶物种,而且还是遗传学研究的好材料。因此,进行龙须菜的基础研究,对更好地开发其经济效益具有重要意义和推动作用。微卫星(SSR)是一种广泛用于遗传多样性、系统发生和种质鉴定等领域的分子标记技术。本论文从三个方面对龙须菜微卫星进行了初步的开发,并对开发出的微卫星进行了多态性检测及种间转移扩增。1.利用生物信息学方法,从公开发表的龙须菜公共核苷酸数据库中寻找多态信息含量丰富的微卫星位点,在筛选出的微卫星位点中选取序列,成功设计合成了5对引物,并在青岛野生龙须菜群体中进行多态性检测。结果显示,5对引物均有扩增产物,但却未检测到多态性。2.从江蓠属其它种的EST数据库中查找微卫星位点,根据Gracilaria changii的EST序列设计合成了10对微卫星引物。通过对青岛野生龙须菜群体中的多态性检测,其中1对引物的扩增产物具有多态性。3.为了获得更多的微卫星引物,通过查找相关文献获得江蓠属其他种(细江蓠Gracilaria gracilis)的4对已知微卫星引物,用在龙须菜上进行引物种间转移扩增研究,有2对引物在龙须菜中有具多态性的扩增产物。实验获得的微卫星引物可用来进行龙须菜种群遗传多样性研究、遗传图谱构建。此外,为比较龙须菜不同品系及江蓠属不同物种在微卫星位点的差异,我们将从龙须菜核酸公共数据库中查找到的微卫星引物在相关品系和相关种间做了转移扩增研究。根据计算得到的各样品间Nei氏遗传相似系数进行聚类分析,结果显示青岛野生型龙须菜样品QD与养殖选育品系样品981、石岛的龙须菜样品SD与龙须岛的龙须菜样品LD之间的遗传相似系数最高;LV与龙须菜之间的差异远远高于种内水平的差异,而成为一种种间水平的差异。
论文目录
相关论文文献
- [1].In Vitro Anti-influenza Virus Activities of Sulfated Polysaccharide Fractions from Gracilaria lemaneiformis[J]. Virologica Sinica 2010(05)
- [2].Structure of reproductive apparatus of Gracilaria/Gracilariopsis lemaneiformis (Gracilariaceae,Rhodophyta)[J]. Science in China(Series C:Life Sciences) 2008(12)
- [3].Identification of a Putative Tetrasporophyte-Specific Gene in Gracilaria lemaneiformis(Gracilariales,Rhodophyte)[J]. Journal of Ocean University of China 2008(03)
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