本文主要研究内容
作者白鑫（2019）在《转BADH基因玉米对土壤理化性质及微生物多样性的影响》一文中研究指出：随着转基因农作物的迅速发展及大量种植,使得越来越多的人开始关注转基因食品的环境安全问题。转甜菜碱醛脱氢酶(Betaine Aldehyde Dehydrogenase,BADH)基因玉米BZ-136能够有效地使玉米耐受盐碱胁迫,从而提高玉米在盐碱土中种植产量。转基因作物的代谢产物可通过根系分泌物或植物残体直接进入土壤,造成一定的环境风险。本研究通过盆栽试验,将玉米分别种植于中性和碱性土壤中,在苗期,拔节期,开花期和成熟期对根际土壤和玉米根部进行取样,测定了转基因玉米BZ-136,亲代非转基因玉米Zheng58和常规自交系玉米U8112根际土壤理化性质、酶活性和细菌群落多样性以及转基因玉米BZ-136和亲代非转基因玉米Zheng58根部丛枝菌根真菌侵染率和根际丛枝菌根真菌群落多样性的差异。本研究获得主要结果如下:(1)转BADH基因玉米对土壤理化性质的影响转BADH基因玉米BZ-136在某些生长时期对土壤电导率、有机碳和全氮等理化性质存在短暂的影响,其差异具有时期性,例如:对于土壤全氮含量,在中性土壤种植条件下,BZ-136在苗期显著低于Zheng58,在拔节期显著高于Zheng58。在碱性土壤种植条件下,BZ-136在拔节期显著低于Zheng58。(2)转BADH基因玉米对土壤酶活性的影响BZ-136根际土壤脲酶活性在碱性土壤中从拔节期到成熟期显著高于非转基因玉米。BZ-136根际土壤碱性磷酸酶活性在中性土壤中开花期和成熟期显著低于非转基因玉米Zheng58和U8112。根际土壤蔗糖酶、过氧化氢酶、酸性磷酸酶和中性磷酸酶活性几乎无显著性差异或仅在某一个生长时期存在显著性差异。(3)转BADH基因玉米对土壤细菌群落多样性的影响基于对根际土壤细菌的16S rRNA高通量测序显示,三个玉米品系细菌群落组成相似。基于OTU水平的NMDS和聚类分析显示,根际土壤中的细菌群落结构受土壤类型和生长时期的影响显著,玉米品系对土壤细菌群落无显著影响。(4)转BADH基因玉米对根部丛枝菌根真菌侵染率的影响在中性和碱性土壤中,BADH转基因玉米BZ-136的菌丝,囊泡和总AMF的侵染率仅在拔节期显著低于非转基因亲本玉米Zheng58(p<0.05)。基于ANOVA分析显示,转BADH基因玉米对根部丛枝菌根真菌的侵染率无显著影响。(5)转BADH基因玉米对土壤丛枝菌根真菌群落多样性的影响基于对根际土壤丛枝菌根真菌的18S rRNA高通量测序显示,BZ-136与Zheng58在属水平上群落组成相似,在种水平上存在一些差异。基于OTU水平的NMDS和聚类分析显示,转BADH基因玉米对根际丛枝菌根真菌的群落结构无显著影响。
Abstract
sui zhao zhuai ji yin nong zuo wu de xun su fa zhan ji da liang chong zhi ,shi de yue lai yue duo de ren kai shi guan zhu zhuai ji yin shi pin de huan jing an quan wen ti 。zhuai tian cai jian quan tuo qing mei (Betaine Aldehyde Dehydrogenase,BADH)ji yin yu mi BZ-136neng gou you xiao de shi yu mi nai shou yan jian xie pai ,cong er di gao yu mi zai yan jian tu zhong chong zhi chan liang 。zhuai ji yin zuo wu de dai xie chan wu ke tong guo gen ji fen bi wu huo zhi wu can ti zhi jie jin ru tu rang ,zao cheng yi ding de huan jing feng xian 。ben yan jiu tong guo pen zai shi yan ,jiang yu mi fen bie chong zhi yu zhong xing he jian xing tu rang zhong ,zai miao ji ,ba jie ji ,kai hua ji he cheng shou ji dui gen ji tu rang he yu mi gen bu jin hang qu yang ,ce ding le zhuai ji yin yu mi BZ-136,qin dai fei zhuai ji yin yu mi Zheng58he chang gui zi jiao ji yu mi U8112gen ji tu rang li hua xing zhi 、mei huo xing he xi jun qun la duo yang xing yi ji zhuai ji yin yu mi BZ-136he qin dai fei zhuai ji yin yu mi Zheng58gen bu cong zhi jun gen zhen jun qin ran lv he gen ji cong zhi jun gen zhen jun qun la duo yang xing de cha yi 。ben yan jiu huo de zhu yao jie guo ru xia :(1)zhuai BADHji yin yu mi dui tu rang li hua xing zhi de ying xiang zhuai BADHji yin yu mi BZ-136zai mou xie sheng chang shi ji dui tu rang dian dao lv 、you ji tan he quan dan deng li hua xing zhi cun zai duan zan de ying xiang ,ji cha yi ju you shi ji xing ,li ru :dui yu tu rang quan dan han liang ,zai zhong xing tu rang chong zhi tiao jian xia ,BZ-136zai miao ji xian zhe di yu Zheng58,zai ba jie ji xian zhe gao yu Zheng58。zai jian xing tu rang chong zhi tiao jian xia ,BZ-136zai ba jie ji xian zhe di yu Zheng58。(2)zhuai BADHji yin yu mi dui tu rang mei huo xing de ying xiang BZ-136gen ji tu rang niao mei huo xing zai jian xing tu rang zhong cong ba jie ji dao cheng shou ji xian zhe gao yu fei zhuai ji yin yu mi 。BZ-136gen ji tu rang jian xing lin suan mei huo xing zai zhong xing tu rang zhong kai hua ji he cheng shou ji xian zhe di yu fei zhuai ji yin yu mi Zheng58he U8112。gen ji tu rang zhe tang mei 、guo yang hua qing mei 、suan xing lin suan mei he zhong xing lin suan mei huo xing ji hu mo xian zhe xing cha yi huo jin zai mou yi ge sheng chang shi ji cun zai xian zhe xing cha yi 。(3)zhuai BADHji yin yu mi dui tu rang xi jun qun la duo yang xing de ying xiang ji yu dui gen ji tu rang xi jun de 16S rRNAgao tong liang ce xu xian shi ,san ge yu mi pin ji xi jun qun la zu cheng xiang shi 。ji yu OTUshui ping de NMDShe ju lei fen xi xian shi ,gen ji tu rang zhong de xi jun qun la jie gou shou tu rang lei xing he sheng chang shi ji de ying xiang xian zhe ,yu mi pin ji dui tu rang xi jun qun la mo xian zhe ying xiang 。(4)zhuai BADHji yin yu mi dui gen bu cong zhi jun gen zhen jun qin ran lv de ying xiang zai zhong xing he jian xing tu rang zhong ,BADHzhuai ji yin yu mi BZ-136de jun si ,nang pao he zong AMFde qin ran lv jin zai ba jie ji xian zhe di yu fei zhuai ji yin qin ben yu mi Zheng58(p<0.05)。ji yu ANOVAfen xi xian shi ,zhuai BADHji yin yu mi dui gen bu cong zhi jun gen zhen jun de qin ran lv mo xian zhe ying xiang 。(5)zhuai BADHji yin yu mi dui tu rang cong zhi jun gen zhen jun qun la duo yang xing de ying xiang ji yu dui gen ji tu rang cong zhi jun gen zhen jun de 18S rRNAgao tong liang ce xu xian shi ,BZ-136yu Zheng58zai shu shui ping shang qun la zu cheng xiang shi ,zai chong shui ping shang cun zai yi xie cha yi 。ji yu OTUshui ping de NMDShe ju lei fen xi xian shi ,zhuai BADHji yin yu mi dui gen ji cong zhi jun gen zhen jun de qun la jie gou mo xian zhe ying xiang 。
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论文作者分别是来自东北农业大学的白鑫，发表于刊物东北农业大学2019-08-27论文，是一篇关于基因论文,玉米论文,风险评价论文,微生物多样性论文,酶活性论文,理化性质论文,东北农业大学2019-08-27论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自东北农业大学2019-08-27论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：基因论文; 玉米论文; 风险评价论文; 微生物多样性论文; 酶活性论文; 理化性质论文; 东北农业大学2019-08-27论文;