林江波:基于转录组测序的铁皮石斛黄酮代谢途径及相关基因解析论文

林江波:基于转录组测序的铁皮石斛黄酮代谢途径及相关基因解析论文

本文主要研究内容

作者林江波,王伟英,邹晖,戴艺民(2019)在《基于转录组测序的铁皮石斛黄酮代谢途径及相关基因解析》一文中研究指出:【目的】分析铁皮石斛Dendrobium officinale黄酮类化合物的生物合成途径及相关基因,为铁皮石斛黄酮类化合物代谢调控、药用价值的开发研究提供参考。【方法】利用Illumina HiSeq 4000测序平台对铁皮石斛2个生长阶段茎、叶进行高通量转录组测序,对组装获得的unigenes进行功能注释和黄酮类化合物的生物合成相关基因解析。【结果】铁皮石斛黄酮类化合物代谢相关Unigenes 48个,涉及14个酶。5个CHS相关Unigenes具有CHSlike保守结构域,活性位点氨基酸残基(Cys-His-Asn)高度保守,丙二酰辅酶A结合位点和产物结合位点的部分氨基酸残基发生变异。CHI(Unigene0013781)属于类型I查尔酮异构酶,异构化6′-羟基查尔酮为5-羟基黄烷酮。表达分析表明,2个生长时期的茎和叶,CHS(Unigene0008250)、 CHI(Unigene0013781)和F3H的表达量都高于CHS(Unigene0012884)和C3′H。【结论】通过对转录组数据分析,共获得铁皮石斛黄酮类化合物代谢相关Unigenes48个,涉及14个酶;5个CHS相关Unigenes中,2个编码查尔酮合酶,3个编码联苄合酶;CHI(Unigene0013781)属于类型I查尔酮异构酶。

Abstract

【mu de 】fen xi tie pi dan hu Dendrobium officinalehuang tong lei hua ge wu de sheng wu ge cheng tu jing ji xiang guan ji yin ,wei tie pi dan hu huang tong lei hua ge wu dai xie diao kong 、yao yong jia zhi de kai fa yan jiu di gong can kao 。【fang fa 】li yong Illumina HiSeq 4000ce xu ping tai dui tie pi dan hu 2ge sheng chang jie duan jing 、xie jin hang gao tong liang zhuai lu zu ce xu ,dui zu zhuang huo de de unigenesjin hang gong neng zhu shi he huang tong lei hua ge wu de sheng wu ge cheng xiang guan ji yin jie xi 。【jie guo 】tie pi dan hu huang tong lei hua ge wu dai xie xiang guan Unigenes 48ge ,she ji 14ge mei 。5ge CHSxiang guan Unigenesju you CHSlikebao shou jie gou yu ,huo xing wei dian an ji suan can ji (Cys-His-Asn)gao du bao shou ,bing er xian fu mei Ajie ge wei dian he chan wu jie ge wei dian de bu fen an ji suan can ji fa sheng bian yi 。CHI(Unigene0013781)shu yu lei xing Icha er tong yi gou mei ,yi gou hua 6′-qiang ji cha er tong wei 5-qiang ji huang wan tong 。biao da fen xi biao ming ,2ge sheng chang shi ji de jing he xie ,CHS(Unigene0008250)、 CHI(Unigene0013781)he F3Hde biao da liang dou gao yu CHS(Unigene0012884)he C3′H。【jie lun 】tong guo dui zhuai lu zu shu ju fen xi ,gong huo de tie pi dan hu huang tong lei hua ge wu dai xie xiang guan Unigenes48ge ,she ji 14ge mei ;5ge CHSxiang guan Unigeneszhong ,2ge bian ma cha er tong ge mei ,3ge bian ma lian bian ge mei ;CHI(Unigene0013781)shu yu lei xing Icha er tong yi gou mei 。

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  • 论文详细介绍

    论文作者分别是来自福建农业学报的林江波,王伟英,邹晖,戴艺民,发表于刊物福建农业学报2019年09期论文,是一篇关于铁皮石斛论文,转录组论文,黄酮论文,功能注释论文,表达论文,代谢论文,福建农业学报2019年09期论文的文章。本文可供学术参考使用,各位学者可以免费参考阅读下载,文章观点不代表本站观点,资料来自福建农业学报2019年09期论文网站,若本站收录的文献无意侵犯了您的著作版权,请联系我们删除。

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    林江波:基于转录组测序的铁皮石斛黄酮代谢途径及相关基因解析论文
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