支柱蓼化学成分研究

论文摘要

蓼属（Polygonum）是蓼科中的药用大属,全世界大约200多种。我国约有120种,其中81种供药用,分布于全国各地,大多具有清热解毒、散结消肿、活血止痛、顺气解痉、收敛止泻、通经利尿等功效。从上世纪80年代开始,蓼属植物的化学成分及药理作用研究,逐渐受到人们的关注。研究发现,蓼属植物主要有黄酮类化合物、蒽醌及其衍生物、二苯乙烯类化合物、糖酯类化合物、木脂素类化合物、甾体以及其它类型的化合物。药理研究表明,本属植物大多数种具有抗炎、抗肿瘤、降脂、降压等生物活性。支柱蓼为民间常用药,但该植物的化学/活性成分研究报道较少,因此我们对该植物进行了较为系统的化学成分研究,以期明确其化学/活性成分。运用溶剂萃取、硅胶柱层析、聚酰胺柱层析、凝胶柱层析以及大孔吸附树脂柱层析、重结晶等分离纯化方法,同时配合硅胶和聚酰胺薄层层析跟踪分析,从支柱蓼乙醇提取物中分离得到11个化合物,并运用核磁共振波谱、质谱与对照品对照的方法鉴定了其中10个化合物的结构,分别为:neohop-13（18）-en-3β-ol（PSE-C1）、neohop-13（18）-en-3β-yl acetat（ePSE-C2a）、hop-17（21）-en-3β-yl acetate（PSE-C2b）、gallic acid（PSE-C3）、sitosterol（PSE-C4）、dauosterol（PSE-C5）、epifriedelinol（PSE-C7）、friedelin（PSE-C8）、neochlorogenic acid n-butyl ester（PSB50-C1）、myricetin-3-O-β-D-glucopyranoside（PSB50-C2）。以上10个化合物中除gallic acid（PSE-C3）外均为首次从支柱蓼中分离得到,其中PSE-C1、PSE-C2a、PSE-C2b、PSB50-C1、PSB50-C2系首次从该属植物中分离得到,PSE-C2a为新的天然产物。本论文还对近二十年来国内外蓼属植物化学成分和生物活性研究进行了综述。

论文目录

摘要

ABSTRACT

第一章蓼属植物化学成分及活性研究进展（综述）

1.1 概述

1.2 化学成分

1.2.1 黄酮类化合物

1.2.2 蒽醌类化合物及其衍生物

1.2.3 二苯乙烯类化合物

1.2.4 其它类型化合物

1.3 生理活性

1.3.1 抗炎、抗氧化和清除自由基作用

1.3.2 抗肿瘤作用

1.3.3 抗菌、杀虫活性

1.3.4 其它生理活性

1.4 小结

第二章支柱蓼化学成分研究

2.1 概述

2.2 实验部分

2.2.1 实验仪器与试剂

2.2.2 植物来源

2.2.3 提取

2.2.4 乙酸乙酯部分（PSE）分离与纯化

2.2.4.1 柱层析分离条件的选择

2.2.4.2 乙酸乙酯部分硅胶柱层析

2.2.4.3 硅胶柱层析洗脱后各流分的处理

2.2.5 正丁醇部分（PSB）的分离与纯化

2.2.5.1 正丁醇部分大孔树脂柱层析

2.2.5.2 正丁醇 50%乙醇部分柱层析条件的选择

2.2.5.3 正丁醇 50%乙醇部分凝胶柱层析

2.2.5.4 凝胶柱层析洗脱后各流分的处理

2.2.6 实验数据

2.2.6.1 化合物PSE-C1

2.2.6.2 化合物PSE-C2

2.2.6.3 化合物PSE-C3

2.2.6.4 化合物PSE-C4

2.2.6.5 化合物PSE-C5

2.2.6.6 化合物PSE-C7

2.2.6.7 化合物PSE-C8

2.2.6.8 化合物PSB50-C1

2.2.6.9 化合物PSB50-C2

2.3 结构鉴定

2.3.1 PSE-C1: neohop-13（18）-en-3β-ol

2.3.2 PSE-C2a: neohop-13（18）-en-3β-yl acetate

2.3.3 PSE-C2b: hop-17（21）-en-3β-yl acetate

2.3.4 PSE-C3: gallic acid

2.3.5 PSE-C4: sitosterol

2.3.6 PSE-C5: dauosterol

2.3.7 PSE-C7: epifriedelinol

2.3.8 PSE-C8: friedelin

2.3.9 PSB50-C1: neochlorogenic acid n-butyl ester

2.3.10 PSB50-C2: myricetin-3-O-β-D-glucopyranoside

2.4 小结

第三章总结与讨论

参考文献

发表论文和参加科研情况说明

附图

致谢

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标签：蓼属论文; 支柱蓼论文; 化学成分论文; 三萜论文; 黄酮论文;

支柱蓼化学成分研究

论文摘要

论文目录

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