本文主要研究内容
作者李洪林（2019）在《贵州地方鸡种隐性白羽与慢羽分子标记研究与应用》一文中研究指出：为了准确鉴定兴义矮脚鸡白羽系的白羽性状,本文根据TYR基因序列和PMEL17序列各分别设计了特异性引物各1对,采用PCR直接测序法鉴定兴义矮脚鸡白羽系(以下简称XB)和罗曼蛋鸡(以下简称LB)的羽色控制基因,建立了地方鸡种显性白羽和隐性白羽分子鉴定方法,开展了罗曼蛋鸡与兴义矮脚白羽系杂交后代中隐性白羽纯合子(以下简称YXB)的鉴定和挑选工作。为了鉴定贵州黄鸡慢羽系、瑶山鸡慢羽系的慢羽性状,根据控制慢羽性状的基因序列,设计特异性引物1对,采用PCR直接测序法对表型鉴别的贵州黄鸡慢羽系(以下简称GHS)和瑶山鸡慢羽系(以下简称YSS)个体进行了分子鉴定,同时设计引物鉴定了慢羽公鸡纯合子,建立了两个鸡种慢羽性状分子标记鉴定方法;统计了贵州黄鸡、瑶山鸡不同世代慢羽羽型的变化规律,探讨了不同羽型对羽毛和体重的影响。结果如下:(1)经分子鉴定,检测到兴义矮脚鸡白羽系中TYR基因196bp的插入片段,测序后比对与原序列一致,未检测到PMEL17基因的160bp插入片段,判定兴义矮脚鸡白羽系为TYR基因控制的隐性白羽鸡,基因型为iicc;检测到罗曼蛋鸡PMEL17基因160bp的插入片段,测序后比对与原序列一致,判定罗曼蛋鸡为PMEL17基因控制的显性白羽,基因型为IICC;对罗曼蛋鸡和兴义矮脚鸡白羽系杂交2代208只鸡的TYR基因进行检测,发现存在IiCC、IICC等8种基因型,未检测到iiCC型。最终鉴定出iicc型个体9只,占总检测数的4.3%,经X~2检验,理论值与实际值相符(P>0.05)。(2)根据控制慢羽性状的基因序列,设计特异性引物1对,经PCR扩增在贵州黄鸡、瑶山鸡慢羽群体中发现1305bp的插入片段,测序比对后与原序列一致,此片段可作为慢羽性状检测的标记片段。以此片段为依据,对表型鉴定的贵州黄鸡慢羽和瑶山鸡慢羽进行分子鉴定,发现贵州黄鸡慢羽系表型鉴定的准确率为98.3%,瑶山鸡慢羽表型鉴定的准确率为97.9%。(3)为了验证慢羽个体分子鉴定的准确性,选择贵州黄鸡8只快羽公鸡,48只慢羽母鸡进行杂交,对146只杂交后代快慢羽性状进行表型鉴定,发现慢羽公鸡67只,快羽母鸡77只,X~2检验,理论值与实际值相符(P>0.05);选择瑶山鸡6只快羽公鸡,36只慢羽母鸡进行杂交,对131只杂交后代快慢羽性状进行表型鉴定,发现慢羽公鸡65只,快羽母鸡66只,X~2检验,理论值与实际值相符(P>0.05)。(4)研究发现,贵州黄鸡、瑶山鸡慢羽羽型在不同世代间变化较大,贵州黄鸡、瑶山鸡慢羽系原代以等长型和倒长型为主导羽型,分别占总慢羽羽型的94.07%和93.67%;经分子标记鉴定后,一世代以倒长型和未长出型为主导羽型,分别占总慢羽羽型数的95.07%和94.98%。为了研究不同羽型对羽毛生长和体重的影响,试验统计了贵州黄鸡0-49日龄羽毛生长和体重发育情况。在主翼羽中发现,28日龄前(除14日龄外),快羽型的主翼羽生长速度明显快于慢羽型的主翼羽(P>0.05);在尾羽中发现,21到42日龄之间,快羽羽型的主尾羽明显长于慢羽其他羽型(P<0.05),而49日龄时,未长出型个体覆主翼羽明显长于倒长型个体(P<0.05);慢羽羽型对体重影响不大,各羽型间差异不显著(P>0.05)。本文建立了隐性白羽和慢羽性状鉴定的分子标记,经验证准确,可用于生产实践。
Abstract
wei le zhun que jian ding xing yi ai jiao ji bai yu ji de bai yu xing zhuang ,ben wen gen ju TYRji yin xu lie he PMEL17xu lie ge fen bie she ji le te yi xing yin wu ge 1dui ,cai yong PCRzhi jie ce xu fa jian ding xing yi ai jiao ji bai yu ji (yi xia jian chen XB)he luo man dan ji (yi xia jian chen LB)de yu se kong zhi ji yin ,jian li le de fang ji chong xian xing bai yu he yin xing bai yu fen zi jian ding fang fa ,kai zhan le luo man dan ji yu xing yi ai jiao bai yu ji za jiao hou dai zhong yin xing bai yu chun ge zi (yi xia jian chen YXB)de jian ding he tiao shua gong zuo 。wei le jian ding gui zhou huang ji man yu ji 、yao shan ji man yu ji de man yu xing zhuang ,gen ju kong zhi man yu xing zhuang de ji yin xu lie ,she ji te yi xing yin wu 1dui ,cai yong PCRzhi jie ce xu fa dui biao xing jian bie de gui zhou huang ji man yu ji (yi xia jian chen GHS)he yao shan ji man yu ji (yi xia jian chen YSS)ge ti jin hang le fen zi jian ding ,tong shi she ji yin wu jian ding le man yu gong ji chun ge zi ,jian li le liang ge ji chong man yu xing zhuang fen zi biao ji jian ding fang fa ;tong ji le gui zhou huang ji 、yao shan ji bu tong shi dai man yu yu xing de bian hua gui lv ,tan tao le bu tong yu xing dui yu mao he ti chong de ying xiang 。jie guo ru xia :(1)jing fen zi jian ding ,jian ce dao xing yi ai jiao ji bai yu ji zhong TYRji yin 196bpde cha ru pian duan ,ce xu hou bi dui yu yuan xu lie yi zhi ,wei jian ce dao PMEL17ji yin de 160bpcha ru pian duan ,pan ding xing yi ai jiao ji bai yu ji wei TYRji yin kong zhi de yin xing bai yu ji ,ji yin xing wei iicc;jian ce dao luo man dan ji PMEL17ji yin 160bpde cha ru pian duan ,ce xu hou bi dui yu yuan xu lie yi zhi ,pan ding luo man dan ji wei PMEL17ji yin kong zhi de xian xing bai yu ,ji yin xing wei IICC;dui luo man dan ji he xing yi ai jiao ji bai yu ji za jiao 2dai 208zhi ji de TYRji yin jin hang jian ce ,fa xian cun zai IiCC、IICCdeng 8chong ji yin xing ,wei jian ce dao iiCCxing 。zui zhong jian ding chu iiccxing ge ti 9zhi ,zhan zong jian ce shu de 4.3%,jing X~2jian yan ,li lun zhi yu shi ji zhi xiang fu (P>0.05)。(2)gen ju kong zhi man yu xing zhuang de ji yin xu lie ,she ji te yi xing yin wu 1dui ,jing PCRkuo zeng zai gui zhou huang ji 、yao shan ji man yu qun ti zhong fa xian 1305bpde cha ru pian duan ,ce xu bi dui hou yu yuan xu lie yi zhi ,ci pian duan ke zuo wei man yu xing zhuang jian ce de biao ji pian duan 。yi ci pian duan wei yi ju ,dui biao xing jian ding de gui zhou huang ji man yu he yao shan ji man yu jin hang fen zi jian ding ,fa xian gui zhou huang ji man yu ji biao xing jian ding de zhun que lv wei 98.3%,yao shan ji man yu biao xing jian ding de zhun que lv wei 97.9%。(3)wei le yan zheng man yu ge ti fen zi jian ding de zhun que xing ,shua ze gui zhou huang ji 8zhi kuai yu gong ji ,48zhi man yu mu ji jin hang za jiao ,dui 146zhi za jiao hou dai kuai man yu xing zhuang jin hang biao xing jian ding ,fa xian man yu gong ji 67zhi ,kuai yu mu ji 77zhi ,X~2jian yan ,li lun zhi yu shi ji zhi xiang fu (P>0.05);shua ze yao shan ji 6zhi kuai yu gong ji ,36zhi man yu mu ji jin hang za jiao ,dui 131zhi za jiao hou dai kuai man yu xing zhuang jin hang biao xing jian ding ,fa xian man yu gong ji 65zhi ,kuai yu mu ji 66zhi ,X~2jian yan ,li lun zhi yu shi ji zhi xiang fu (P>0.05)。(4)yan jiu fa xian ,gui zhou huang ji 、yao shan ji man yu yu xing zai bu tong shi dai jian bian hua jiao da ,gui zhou huang ji 、yao shan ji man yu ji yuan dai yi deng chang xing he dao chang xing wei zhu dao yu xing ,fen bie zhan zong man yu yu xing de 94.07%he 93.67%;jing fen zi biao ji jian ding hou ,yi shi dai yi dao chang xing he wei chang chu xing wei zhu dao yu xing ,fen bie zhan zong man yu yu xing shu de 95.07%he 94.98%。wei le yan jiu bu tong yu xing dui yu mao sheng chang he ti chong de ying xiang ,shi yan tong ji le gui zhou huang ji 0-49ri ling yu mao sheng chang he ti chong fa yo qing kuang 。zai zhu yi yu zhong fa xian ,28ri ling qian (chu 14ri ling wai ),kuai yu xing de zhu yi yu sheng chang su du ming xian kuai yu man yu xing de zhu yi yu (P>0.05);zai wei yu zhong fa xian ,21dao 42ri ling zhi jian ,kuai yu yu xing de zhu wei yu ming xian chang yu man yu ji ta yu xing (P<0.05),er 49ri ling shi ,wei chang chu xing ge ti fu zhu yi yu ming xian chang yu dao chang xing ge ti (P<0.05);man yu yu xing dui ti chong ying xiang bu da ,ge yu xing jian cha yi bu xian zhe (P>0.05)。ben wen jian li le yin xing bai yu he man yu xing zhuang jian ding de fen zi biao ji ,jing yan zheng zhun que ,ke yong yu sheng chan shi jian 。
论文参考文献
论文详细介绍
论文作者分别是来自贵州大学的李洪林，发表于刊物贵州大学2019-07-16论文，是一篇关于贵州黄鸡论文,瑶山鸡论文,兴义矮脚鸡白羽系论文,分子标记论文,隐性白羽论文,快慢羽论文,贵州大学2019-07-16论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自贵州大学2019-07-16论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：贵州黄鸡论文; 瑶山鸡论文; 兴义矮脚鸡白羽系论文; 分子标记论文; 隐性白羽论文; 快慢羽论文; 贵州大学2019-07-16论文;