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作者梁峰豪,郎丽娜,刘亚萍,王静,张鹤,徐爱遐,黄镇（2019）在《抗根肿病种质资源的筛选及油菜抗根肿病种质的合成》一文中研究指出：为了筛选十字花科抗根肿病种质,将抗性基因转育到甘蓝型油菜中,利用四川成都根肿菌4号生理小种对西北农林科技大学油菜课题组搜集提供的30份十字花科种质进行接种筛选,并对病情指数进行显著性差异分析、欧式聚类分析及发病率与病情指数的相关性分析。最终获得7份高抗(HR)材料,包括3份白菜、3份芜菁和1份甘蓝,2份中感(MS)材料,2份感病(S)材料和19份高感(HS)材料,其中高感材料均为油菜;这30份材料的平均发病率为69. 13%,平均发病指数为51. 45;抗、感材料病情指数之间存在极显著差异,欧式聚类分析与病情指数分析结果相一致,发病率与病情指数之间存在线性相关。将筛选获得的抗病芜菁德白瑞与感病甘蓝型油菜育种骨干亲本徐09进行远缘杂交,利用形态学、抗性鉴定、SSR分子标记等方法对远缘杂交的F1进行了真假杂种鉴定。结果发现,获得的3株F1杂种叶色浓绿,叶片肥厚,叶缘刺毛较少,叶型修长呈椭圆形,整体性状表现介于两亲本之间且偏向于母本,接菌后无发病症状,并用筛选得到的15对具有较好扩增多态性的SSR引物扫描3株杂种及双亲,带型均同时表现父母本带型,最终确定这3份F1杂种均为真杂种。筛选的7份高抗材料和3份真杂种材料可以为根肿病抗性机理研究及油菜抗根肿病新品种的选育提供材料基础。
Abstract
wei le shai shua shi zi hua ke kang gen zhong bing chong zhi ,jiang kang xing ji yin zhuai yo dao gan lan xing you cai zhong ,li yong si chuan cheng dou gen zhong jun 4hao sheng li xiao chong dui xi bei nong lin ke ji da xue you cai ke ti zu sou ji di gong de 30fen shi zi hua ke chong zhi jin hang jie chong shai shua ,bing dui bing qing zhi shu jin hang xian zhe xing cha yi fen xi 、ou shi ju lei fen xi ji fa bing lv yu bing qing zhi shu de xiang guan xing fen xi 。zui zhong huo de 7fen gao kang (HR)cai liao ,bao gua 3fen bai cai 、3fen wu jing he 1fen gan lan ,2fen zhong gan (MS)cai liao ,2fen gan bing (S)cai liao he 19fen gao gan (HS)cai liao ,ji zhong gao gan cai liao jun wei you cai ;zhe 30fen cai liao de ping jun fa bing lv wei 69. 13%,ping jun fa bing zhi shu wei 51. 45;kang 、gan cai liao bing qing zhi shu zhi jian cun zai ji xian zhe cha yi ,ou shi ju lei fen xi yu bing qing zhi shu fen xi jie guo xiang yi zhi ,fa bing lv yu bing qing zhi shu zhi jian cun zai xian xing xiang guan 。jiang shai shua huo de de kang bing wu jing de bai rui yu gan bing gan lan xing you cai yo chong gu gan qin ben xu 09jin hang yuan yuan za jiao ,li yong xing tai xue 、kang xing jian ding 、SSRfen zi biao ji deng fang fa dui yuan yuan za jiao de F1jin hang le zhen jia za chong jian ding 。jie guo fa xian ,huo de de 3zhu F1za chong xie se nong lu ,xie pian fei hou ,xie yuan ci mao jiao shao ,xie xing xiu chang cheng tuo yuan xing ,zheng ti xing zhuang biao xian jie yu liang qin ben zhi jian ju pian xiang yu mu ben ,jie jun hou mo fa bing zheng zhuang ,bing yong shai shua de dao de 15dui ju you jiao hao kuo zeng duo tai xing de SSRyin wu sao miao 3zhu za chong ji shuang qin ,dai xing jun tong shi biao xian fu mu ben dai xing ,zui zhong que ding zhe 3fen F1za chong jun wei zhen za chong 。shai shua de 7fen gao kang cai liao he 3fen zhen za chong cai liao ke yi wei gen zhong bing kang xing ji li yan jiu ji you cai kang gen zhong bing xin pin chong de shua yo di gong cai liao ji chu 。
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论文作者分别是来自华北农学报的梁峰豪,郎丽娜,刘亚萍,王静,张鹤,徐爱遐,黄镇，发表于刊物华北农学报2019年01期论文，是一篇关于根肿病论文,十字花科抗性论文,种质筛选论文,远缘杂交论文,真假杂种鉴定论文,甘蓝型油菜论文,华北农学报2019年01期论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自华北农学报2019年01期论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：根肿病论文; 十字花科抗性论文; 种质筛选论文; 远缘杂交论文; 真假杂种鉴定论文; 甘蓝型油菜论文; 华北农学报2019年01期论文;