论文摘要
背景与目的:小分子干扰 RNA（siRNAs）可以高效、特异地阻断体内同源基因的表达,促进同源 mRNA 降解,称为 RNA 干扰（RNAi）。本研究旨在探讨 Smad7 基因的 siRNAs 是否能抑制基因的表达。 方法:我们利用 RNA 干扰技术,设计并合成了针对 Smad7 基因的siRNAs,用脂质体转染法瞬时转染 BEP2D 和 BERP35T2 细胞,用Northern blot 法检测 RNAi 效应;同时设计并合成了绿色荧光蛋白（GFP）的 siRNAs,瞬时转染稳定表达绿色荧光蛋白的 BERP35T2 细胞,检测荧光强度有无改变。 结果:RNA 干扰技术能明显抑制 Smad7 基因的表达,并能显著减弱对照组绿色荧光的表达强度。 结论:RNA 干扰技术能高效抑制 Smad7 基因的表达,为进一步研究 Smad7 基因功能及 TGF-β 信号转导通路奠定了基础。
论文目录
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