本文主要研究内容
作者向明,李媛媛,郭乾鹏,任亚雪,梁世忠,黄怡（2019）在《屎肠球菌对哺乳期仔猪结肠微生物群落优势门属的影响》一文中研究指出：【目的】分析屎肠球菌(Enterococcus faecium)活菌对哺乳期仔猪结肠微生物群落优势门属的持续影响,为揭示益生菌调节仔猪肠道健康的作用机制及人为调控仔猪肠道微生物以促进其健康提供参考依据。【方法】给健康新生仔猪灌服无菌脱脂乳(对照组)或屎肠球菌制剂(益生菌组),持续灌服6 d,分别采集7和21日龄仔猪结肠内容物,提取总细菌DNA后进行PCR扩增,采用Illumina MiSeq高通量测序技术检测16S rRNA序列的V3～V4可变区,分析肠道微生物群落α多样性及其在门和属分类水平上的结构特点。【结果】给新生仔猪灌服屎肠球菌并不影响7和21日龄仔猪结肠微生物群落α多样性。与对照组相比,益生菌组7日龄仔猪结肠微生物群落优势门的相对丰度无显著变化(P>0.05,下同),21日龄仔猪拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes)的相对丰度也无显著差异,但变形杆菌门(Proteobacteria)相对丰度呈显著增加趋势(P<0.05,下同);在属水平上,益生菌组7日龄仔猪结肠微生物群落中相对丰度变化明显的优势属均隶属于拟杆菌门,其中,普雷沃氏菌属(Prevotella)相对丰度显著增加,而拟杆菌属(Bacteroides)相对丰度极显著减少(P<0.01,下同),丁酸单胞菌属(Butyricimonas)相对丰度显著减少;21日龄仔猪结肠微生物群落中66.67%优势属的相对丰度呈减少趋势,其中以厚壁菌门4个属的变化尤其明显,即巨型球菌属(Megasphaera)相对丰度极显著减少,乳杆菌属(Lactobacillus)、产琥珀酸菌属(Succiniclasticum)和粪杆菌属(Faecalibacterium)相对丰度显著减少。【结论】新生仔猪口服屎肠球菌能对其结肠微生物群落优势门或属组成产生持续影响,表现为促进有益于结肠健康菌属的生长,而抑制具有潜在致病性菌属的增殖,为今后定向研究调控仔猪肠道健康的肠道共生微生物提供明确范围。
Abstract
【mu de 】fen xi shi chang qiu jun (Enterococcus faecium)huo jun dui bu ru ji zai zhu jie chang wei sheng wu qun la you shi men shu de chi xu ying xiang ,wei jie shi yi sheng jun diao jie zai zhu chang dao jian kang de zuo yong ji zhi ji ren wei diao kong zai zhu chang dao wei sheng wu yi cu jin ji jian kang di gong can kao yi ju 。【fang fa 】gei jian kang xin sheng zai zhu guan fu mo jun tuo zhi ru (dui zhao zu )huo shi chang qiu jun zhi ji (yi sheng jun zu ),chi xu guan fu 6 d,fen bie cai ji 7he 21ri ling zai zhu jie chang nei rong wu ,di qu zong xi jun DNAhou jin hang PCRkuo zeng ,cai yong Illumina MiSeqgao tong liang ce xu ji shu jian ce 16S rRNAxu lie de V3～V4ke bian ou ,fen xi chang dao wei sheng wu qun la αduo yang xing ji ji zai men he shu fen lei shui ping shang de jie gou te dian 。【jie guo 】gei xin sheng zai zhu guan fu shi chang qiu jun bing bu ying xiang 7he 21ri ling zai zhu jie chang wei sheng wu qun la αduo yang xing 。yu dui zhao zu xiang bi ,yi sheng jun zu 7ri ling zai zhu jie chang wei sheng wu qun la you shi men de xiang dui feng du mo xian zhe bian hua (P>0.05,xia tong ),21ri ling zai zhu ni gan jun men (Bacteroidetes)he hou bi jun men (Firmicutes)de xiang dui feng du ye mo xian zhe cha yi ,dan bian xing gan jun men (Proteobacteria)xiang dui feng du cheng xian zhe zeng jia qu shi (P<0.05,xia tong );zai shu shui ping shang ,yi sheng jun zu 7ri ling zai zhu jie chang wei sheng wu qun la zhong xiang dui feng du bian hua ming xian de you shi shu jun li shu yu ni gan jun men ,ji zhong ,pu lei wo shi jun shu (Prevotella)xiang dui feng du xian zhe zeng jia ,er ni gan jun shu (Bacteroides)xiang dui feng du ji xian zhe jian shao (P<0.01,xia tong ),ding suan chan bao jun shu (Butyricimonas)xiang dui feng du xian zhe jian shao ;21ri ling zai zhu jie chang wei sheng wu qun la zhong 66.67%you shi shu de xiang dui feng du cheng jian shao qu shi ,ji zhong yi hou bi jun men 4ge shu de bian hua you ji ming xian ,ji ju xing qiu jun shu (Megasphaera)xiang dui feng du ji xian zhe jian shao ,ru gan jun shu (Lactobacillus)、chan hu po suan jun shu (Succiniclasticum)he fen gan jun shu (Faecalibacterium)xiang dui feng du xian zhe jian shao 。【jie lun 】xin sheng zai zhu kou fu shi chang qiu jun neng dui ji jie chang wei sheng wu qun la you shi men huo shu zu cheng chan sheng chi xu ying xiang ,biao xian wei cu jin you yi yu jie chang jian kang jun shu de sheng chang ,er yi zhi ju you qian zai zhi bing xing jun shu de zeng shi ,wei jin hou ding xiang yan jiu diao kong zai zhu chang dao jian kang de chang dao gong sheng wei sheng wu di gong ming que fan wei 。
论文参考文献
论文详细介绍
论文作者分别是来自南方农业学报的向明,李媛媛,郭乾鹏,任亚雪,梁世忠,黄怡，发表于刊物南方农业学报2019年03期论文，是一篇关于仔猪论文,屎肠球菌论文,结肠论文,微生物群落论文,多样性论文,高通量测序论文,南方农业学报2019年03期论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自南方农业学报2019年03期论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：仔猪论文; 屎肠球菌论文; 结肠论文; 微生物群落论文; 多样性论文; 高通量测序论文; 南方农业学报2019年03期论文;