本文主要研究内容
作者（2019）在《Fast mapping of a chlorophyll b synthesis-deficiency gene in barley(Hordeum vulgare L.) via bulked-segregant analysis with reduced-representation sequencing》一文中研究指出：Bulked-segregant analysis coupled with next-generation sequencing（BSA-seq） has emerged as an efficient tool for genetic mapping of single genes or major quantitative trait loci controlling（agronomic） traits of interest. However, such a mapping-by-sequencing approach usually relies on deep sequencing and advanced statistical methods. Application of BSA-Seq based on construction of reduced-representation libraries and allele frequency analysis permitted anchoring the barley pale-green（pg） gene on chromosome 3 HL. With further marker-assisted validation, pg was mapped to a 3.9 Mb physical-map interval. In the pg mutant a complete deletion of chlorophyllide a oxygenase（HvCAO） gene was identified.Because the product of this gene converts Chl a to Chl b, the pg mutant is deficient in Chl b.An independent Chl b-less mutant line M44372 carried a nonsynonymous substitution（F263 L） in the C domain of HvCAO. The study demonstrates an optimized pooling strategy for fast mapping of agronomically important genes using a segregating population.
Abstract
Bulked-segregant analysis coupled with next-generation sequencing（BSA-seq） has emerged as an efficient tool for genetic mapping of single genes or major quantitative trait loci controlling（agronomic） traits of interest. However, such a mapping-by-sequencing approach usually relies on deep sequencing and advanced statistical methods. Application of BSA-Seq based on construction of reduced-representation libraries and allele frequency analysis permitted anchoring the barley pale-green（pg） gene on chromosome 3 HL. With further marker-assisted validation, pg was mapped to a 3.9 Mb physical-map interval. In the pg mutant a complete deletion of chlorophyllide a oxygenase（HvCAO） gene was identified.Because the product of this gene converts Chl a to Chl b, the pg mutant is deficient in Chl b.An independent Chl b-less mutant line M44372 carried a nonsynonymous substitution（F263 L） in the C domain of HvCAO. The study demonstrates an optimized pooling strategy for fast mapping of agronomically important genes using a segregating population.
论文参考文献
论文详细介绍
论文作者分别是来自The Crop Journal的，发表于刊物The Crop Journal2019年01期论文，是一篇关于,The Crop Journal2019年01期论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自The Crop Journal2019年01期论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。