论文摘要
水稻具有重要的经济价值,是植物发育与分子生物学研究的模式植物。随着水稻基因组计划的完成,水稻发育的研究已逐步深入到分子水平。而精细胞作为雄配子在水稻双受精过程中占据着非常重要的地位。来源于同一个花粉的一对精细胞经花粉管传递进入胚囊中,一个与卵细胞融合产生合子,最终发育成胚,另一个与中央细胞融合发育成胚乳。因此,深入研究水稻精细胞发育相关基因的表达具有重要的理论意义和应用价值。RSSG8基因和RSSG58基因就是本实验室从水稻（Oryza sativa L.）精细胞cDNA文库中差减得到的两条精细胞优势表达基因。 本研究利用RSSG58基因cDNA全序列与NCBI中的粳稻基因组文库进行比对、定位;结合生物信息学方法分析预测基因上游的启动子序列。在此基础上设计引物,以粳稻日本晴Oryza sativa （japonica cultivar-group）黄化苗总DNA为模板采用DNA聚合酶链式反应（PCR）的方法扩增出基因上游1093bp和462bp两个不同长度的启动子缺失片段（分别命名为JP58B和JP58S）,测序结果显示其具有大多数高等植物启动子的保守元件。进一步构建该启动子片段的植物表达载体pBI121-JP58B和pBI121-JP58S,瞬时表达结果显示两个启动子片段对报告基因GUS均具有较强的启动活性。 对RSSG8基因结构进行生物信息学分析。发现RSSG8基因在水稻基因组中以单拷贝形式存在;含有众多的酶切位点;定位于水稻12号染色体上;但其cDNA序列的2046～3762bp区段在GenBank收录的水稻基因组现有数据库中没找到同源序列。RSSG8开放读框编码的蛋白质全长1161个氨基酸;预测其没有明显的疏水性;是一个分子量为129.7kD,等电点为8.305的可溶性蛋白;N-端没有信
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