本文主要研究内容
作者惠建超（2019）在《高活性核桃内生真菌筛选及其代谢产物抑菌机理研究（Ⅱ）》一文中研究指出：核桃内生真菌具有丰富的生物多样性,为了更好地利用这种微生物资源,本研究在核桃内生真菌活性菌株筛选、发酵条件优化、代谢产物分离纯化,以及抑菌机理等方面进行研究,主要结果如下:（1）采用生长速率法,以6种常见植物病原菌为指示菌,对16株核桃内生真菌进行活性筛选。结果表明,1株来源于良种核桃树根部的内生真菌G3,其发酵产物对6种病原菌均表现出一定的抑菌活性。其中对苹果腐烂病原菌抑制率达到100%,完全抑制了病原菌菌丝生长;对水稻纹枯、核桃炭疽病原菌抑制率均大于75%。通过形态学特征观察结合分子生物学鉴定,G3确定为弯角镰刀菌Fusarium camptoceras。（2）以接种量、发酵温度和发酵天数为影响因子,以G3发酵产物对苹果腐烂病原菌的抑制率为评价指标,通过单因素试验、正交试验对核桃内生真菌G3的发酵工艺条件进行优化。结果表明,G3发酵的最优工艺为:接种量10%,发酵温度28℃,发酵时间30 d;此工艺条件下,G3发酵产物对苹果腐烂病原菌抑制率为85.17%。影响因子对抑制率的影响依次为:发酵温度>发酵天数>接种量。（3）采用液液分配方法,对核桃内生真菌G3固体发酵产物的乙醇提取物依次用石油醚、乙酸乙酯、正丁醇分别进行萃取。结果表明,正丁醇相和乙酸乙酯相对苹果腐烂病原菌抑制的IC50值分别为3.3 mg/mL和4.3 mg/mL;结合各萃取相质量及其对苹果腐烂病原菌抑制效果综合考虑,选择正丁醇相和乙酸乙酯相作为深入研究的活性萃取相。（4）通过对各萃取相化学成分预试验,结果显示,正丁醇相主要含有有机酸类物质,乙酸乙酯相主要为酚类物质,推测G3发酵产物活性物质可能为有机酸与酚类物质。对乙酸乙酯萃取相进行柱色谱分离获得高活性组分Fry2-2,供试浓度为1 mg/mL时,对苹果腐烂病原菌抑制率达到89.36%;GC-MS分析共检测到15种化合物,主要为胺类、脂类、酚类与烷烃类。对Fry2-2进一步分离,在Fry2-2-2组分中析出化合物A,经过重结晶纯化和核磁共振等检测,化合物A鉴定为:2,2’-亚甲基双[6-（1,1-二甲基乙基）]-4-甲基-苯酚,抑制苹果腐烂病原菌的IC50值为0.11 mg/mL。（5）在体外和体内条件下,对G3高活性组分抑制苹果腐烂病的机理进行研究。结果表明:高活性组分处理下,病原菌菌丝出现破损、撕裂,或者顶端形成纺锤形,使其生长异常,菌丝量减少;病原菌菌液的电导率增加、麦角甾醇含量降低、几丁质酶活性增加;感病苹果的体内防御酶SOD、PPO、POD活性均明显升高,高活性组分与5 mmol/L水杨酸两者处理效果相近,防御酶活性都呈现先升高后降低的趋势,并能够明显抑制苹果腐烂病斑的扩展,最大抑制率为77.61%。
Abstract
he tao nei sheng zhen jun ju you feng fu de sheng wu duo yang xing ,wei le geng hao de li yong zhe chong wei sheng wu zi yuan ,ben yan jiu zai he tao nei sheng zhen jun huo xing jun zhu shai shua 、fa jiao tiao jian you hua 、dai xie chan wu fen li chun hua ,yi ji yi jun ji li deng fang mian jin hang yan jiu ,zhu yao jie guo ru xia :（1）cai yong sheng chang su lv fa ,yi 6chong chang jian zhi wu bing yuan jun wei zhi shi jun ,dui 16zhu he tao nei sheng zhen jun jin hang huo xing shai shua 。jie guo biao ming ,1zhu lai yuan yu liang chong he tao shu gen bu de nei sheng zhen jun G3,ji fa jiao chan wu dui 6chong bing yuan jun jun biao xian chu yi ding de yi jun huo xing 。ji zhong dui ping guo fu lan bing yuan jun yi zhi lv da dao 100%,wan quan yi zhi le bing yuan jun jun si sheng chang ;dui shui dao wen ku 、he tao tan ju bing yuan jun yi zhi lv jun da yu 75%。tong guo xing tai xue te zheng guan cha jie ge fen zi sheng wu xue jian ding ,G3que ding wei wan jiao lian dao jun Fusarium camptoceras。（2）yi jie chong liang 、fa jiao wen du he fa jiao tian shu wei ying xiang yin zi ,yi G3fa jiao chan wu dui ping guo fu lan bing yuan jun de yi zhi lv wei ping jia zhi biao ,tong guo chan yin su shi yan 、zheng jiao shi yan dui he tao nei sheng zhen jun G3de fa jiao gong yi tiao jian jin hang you hua 。jie guo biao ming ,G3fa jiao de zui you gong yi wei :jie chong liang 10%,fa jiao wen du 28℃,fa jiao shi jian 30 d;ci gong yi tiao jian xia ,G3fa jiao chan wu dui ping guo fu lan bing yuan jun yi zhi lv wei 85.17%。ying xiang yin zi dui yi zhi lv de ying xiang yi ci wei :fa jiao wen du >fa jiao tian shu >jie chong liang 。（3）cai yong ye ye fen pei fang fa ,dui he tao nei sheng zhen jun G3gu ti fa jiao chan wu de yi chun di qu wu yi ci yong dan you mi 、yi suan yi zhi 、zheng ding chun fen bie jin hang cui qu 。jie guo biao ming ,zheng ding chun xiang he yi suan yi zhi xiang dui ping guo fu lan bing yuan jun yi zhi de IC50zhi fen bie wei 3.3 mg/mLhe 4.3 mg/mL;jie ge ge cui qu xiang zhi liang ji ji dui ping guo fu lan bing yuan jun yi zhi xiao guo zeng ge kao lv ,shua ze zheng ding chun xiang he yi suan yi zhi xiang zuo wei shen ru yan jiu de huo xing cui qu xiang 。（4）tong guo dui ge cui qu xiang hua xue cheng fen yu shi yan ,jie guo xian shi ,zheng ding chun xiang zhu yao han you you ji suan lei wu zhi ,yi suan yi zhi xiang zhu yao wei fen lei wu zhi ,tui ce G3fa jiao chan wu huo xing wu zhi ke neng wei you ji suan yu fen lei wu zhi 。dui yi suan yi zhi cui qu xiang jin hang zhu se pu fen li huo de gao huo xing zu fen Fry2-2,gong shi nong du wei 1 mg/mLshi ,dui ping guo fu lan bing yuan jun yi zhi lv da dao 89.36%;GC-MSfen xi gong jian ce dao 15chong hua ge wu ,zhu yao wei an lei 、zhi lei 、fen lei yu wan ting lei 。dui Fry2-2jin yi bu fen li ,zai Fry2-2-2zu fen zhong xi chu hua ge wu A,jing guo chong jie jing chun hua he he ci gong zhen deng jian ce ,hua ge wu Ajian ding wei :2,2’-ya jia ji shuang [6-（1,1-er jia ji yi ji ）]-4-jia ji -ben fen ,yi zhi ping guo fu lan bing yuan jun de IC50zhi wei 0.11 mg/mL。（5）zai ti wai he ti nei tiao jian xia ,dui G3gao huo xing zu fen yi zhi ping guo fu lan bing de ji li jin hang yan jiu 。jie guo biao ming :gao huo xing zu fen chu li xia ,bing yuan jun jun si chu xian po sun 、si lie ,huo zhe ding duan xing cheng fang chui xing ,shi ji sheng chang yi chang ,jun si liang jian shao ;bing yuan jun jun ye de dian dao lv zeng jia 、mai jiao zai chun han liang jiang di 、ji ding zhi mei huo xing zeng jia ;gan bing ping guo de ti nei fang yu mei SOD、PPO、PODhuo xing jun ming xian sheng gao ,gao huo xing zu fen yu 5 mmol/Lshui yang suan liang zhe chu li xiao guo xiang jin ,fang yu mei huo xing dou cheng xian xian sheng gao hou jiang di de qu shi ,bing neng gou ming xian yi zhi ping guo fu lan bing ban de kuo zhan ,zui da yi zhi lv wei 77.61%。
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论文作者分别是来自西北农林科技大学的惠建超，发表于刊物西北农林科技大学2019-07-11论文，是一篇关于核桃论文,内生真菌论文,正交试验论文,西北农林科技大学2019-07-11论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自西北农林科技大学2019-07-11论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：核桃论文; 内生真菌论文; 正交试验论文; 西北农林科技大学2019-07-11论文;