本文主要研究内容
作者刘邦民（2019）在《腐胺调控仔猪肠黏膜上皮细胞增殖和损伤修复的机制研究》一文中研究指出：肠道是猪营养物质消化吸收和抵御外界病菌侵害的重要器官。断奶等应激引起的肠黏膜损伤会造成仔猪腹泻和生长性能下降。探索肠黏膜损伤修复的生物学机制对提高养猪生产效益具有重大科学意义。腐胺作为一种多胺类小分子具有类生长因子功能,能促进肠细胞增殖分化和损伤修复,但其作用机制尚不清楚。本研究通过体内体外试验来探究腐胺促进仔猪肠道损伤修复的分子机制。本研究共分为四个部分:(1)选取72头体重相近、健康的23日龄断奶仔猪(杜洛克×长白×大白),随机分成4个处理,每个处理6个重复,每个重复3头猪。试验周期14天,试验期间饲喂不同处理日粮,包括基础日粮(对照组)以及分别添加0.1%、0.2%和0.3%的腐胺二盐酸盐的日粮,研究日粮中添加不同水平腐胺对断奶仔猪生长性能、腹泻率和肠道形态的影响;(2)以新生仔猪空肠黏膜上皮细胞IPEC-J2为模型,通过阻断细胞内腐胺的生物合成或添加外源腐胺,研究腐胺对肠黏膜上皮细胞增殖和迁移的影响及其发挥作用的关键信号通路中节点基因MAPK/ERK和FAK表达的影响;(3)通过LPS刺激IPEC-J2建立肠黏膜上皮细胞炎症模型,观察炎症反应对细胞增殖和迁移的影响,以及腐胺对仔猪肠黏膜上皮细胞炎症反应的缓解作用;(4)通过测定饲养试验中采集的断奶仔猪空肠黏膜相关蛋白和基因的表达探究日粮中添加腐胺对断奶仔猪小肠黏膜迁移和增殖及炎症反应相关通路关键蛋白的调控。试验结果发现,日粮添加0.2%腐胺二盐酸盐可以显著降低断奶仔猪腹泻率,并且对小肠肠道形态有保护作用,0.2%腐胺二盐酸盐添加组显著提高ZO-1和Occludin的基因表达,对Claudin-1也有促进表达的作用,但差异不显著(P>0.05)。培养基中添加外源腐胺可以显著促进小肠黏膜细胞IPEC-J2的增殖和迁移,其中当腐胺添加浓度为200μmol/L时效果最佳。培养基中添加外源腐胺可以有效缓解DFMO对小肠黏膜细胞IPEC-J2增殖和迁移的抑制作用。并且进一步发现,腐胺主要是通过上调ERK蛋白和FAK蛋白的磷酸化来分别促进IPEC-J2细胞的增殖和迁移。LPS显著提高了IPEC-J2细胞中炎性因子TNF-α、IL-8、IL-6的表达,并且抑制了细胞的增殖和迁移。添加腐胺有效缓解LPS对细胞的增殖和迁移的抑制作用。日粮添加0.2%腐胺二盐酸盐也显著降低了断奶仔猪小肠黏膜中TNF-α、IL-8、IL-6及NF-κB P65的基因表达。我们的试验结果为断奶仔猪肠道健康提供了新的营养调控策略,对其它物种幼畜肠道发育的营养调控也有重要借鉴意义。
Abstract
chang dao shi zhu ying yang wu zhi xiao hua xi shou he di yu wai jie bing jun qin hai de chong yao qi guan 。duan nai deng ying ji yin qi de chang nian mo sun shang hui zao cheng zai zhu fu xie he sheng chang xing neng xia jiang 。tan suo chang nian mo sun shang xiu fu de sheng wu xue ji zhi dui di gao yang zhu sheng chan xiao yi ju you chong da ke xue yi yi 。fu an zuo wei yi chong duo an lei xiao fen zi ju you lei sheng chang yin zi gong neng ,neng cu jin chang xi bao zeng shi fen hua he sun shang xiu fu ,dan ji zuo yong ji zhi shang bu qing chu 。ben yan jiu tong guo ti nei ti wai shi yan lai tan jiu fu an cu jin zai zhu chang dao sun shang xiu fu de fen zi ji zhi 。ben yan jiu gong fen wei si ge bu fen :(1)shua qu 72tou ti chong xiang jin 、jian kang de 23ri ling duan nai zai zhu (du luo ke ×chang bai ×da bai ),sui ji fen cheng 4ge chu li ,mei ge chu li 6ge chong fu ,mei ge chong fu 3tou zhu 。shi yan zhou ji 14tian ,shi yan ji jian si wei bu tong chu li ri liang ,bao gua ji chu ri liang (dui zhao zu )yi ji fen bie tian jia 0.1%、0.2%he 0.3%de fu an er yan suan yan de ri liang ,yan jiu ri liang zhong tian jia bu tong shui ping fu an dui duan nai zai zhu sheng chang xing neng 、fu xie lv he chang dao xing tai de ying xiang ;(2)yi xin sheng zai zhu kong chang nian mo shang pi xi bao IPEC-J2wei mo xing ,tong guo zu duan xi bao nei fu an de sheng wu ge cheng huo tian jia wai yuan fu an ,yan jiu fu an dui chang nian mo shang pi xi bao zeng shi he qian yi de ying xiang ji ji fa hui zuo yong de guan jian xin hao tong lu zhong jie dian ji yin MAPK/ERKhe FAKbiao da de ying xiang ;(3)tong guo LPSci ji IPEC-J2jian li chang nian mo shang pi xi bao yan zheng mo xing ,guan cha yan zheng fan ying dui xi bao zeng shi he qian yi de ying xiang ,yi ji fu an dui zai zhu chang nian mo shang pi xi bao yan zheng fan ying de huan jie zuo yong ;(4)tong guo ce ding si yang shi yan zhong cai ji de duan nai zai zhu kong chang nian mo xiang guan dan bai he ji yin de biao da tan jiu ri liang zhong tian jia fu an dui duan nai zai zhu xiao chang nian mo qian yi he zeng shi ji yan zheng fan ying xiang guan tong lu guan jian dan bai de diao kong 。shi yan jie guo fa xian ,ri liang tian jia 0.2%fu an er yan suan yan ke yi xian zhe jiang di duan nai zai zhu fu xie lv ,bing ju dui xiao chang chang dao xing tai you bao hu zuo yong ,0.2%fu an er yan suan yan tian jia zu xian zhe di gao ZO-1he Occludinde ji yin biao da ,dui Claudin-1ye you cu jin biao da de zuo yong ,dan cha yi bu xian zhe (P>0.05)。pei yang ji zhong tian jia wai yuan fu an ke yi xian zhe cu jin xiao chang nian mo xi bao IPEC-J2de zeng shi he qian yi ,ji zhong dang fu an tian jia nong du wei 200μmol/Lshi xiao guo zui jia 。pei yang ji zhong tian jia wai yuan fu an ke yi you xiao huan jie DFMOdui xiao chang nian mo xi bao IPEC-J2zeng shi he qian yi de yi zhi zuo yong 。bing ju jin yi bu fa xian ,fu an zhu yao shi tong guo shang diao ERKdan bai he FAKdan bai de lin suan hua lai fen bie cu jin IPEC-J2xi bao de zeng shi he qian yi 。LPSxian zhe di gao le IPEC-J2xi bao zhong yan xing yin zi TNF-α、IL-8、IL-6de biao da ,bing ju yi zhi le xi bao de zeng shi he qian yi 。tian jia fu an you xiao huan jie LPSdui xi bao de zeng shi he qian yi de yi zhi zuo yong 。ri liang tian jia 0.2%fu an er yan suan yan ye xian zhe jiang di le duan nai zai zhu xiao chang nian mo zhong TNF-α、IL-8、IL-6ji NF-κB P65de ji yin biao da 。wo men de shi yan jie guo wei duan nai zai zhu chang dao jian kang di gong le xin de ying yang diao kong ce lve ,dui ji ta wu chong you chu chang dao fa yo de ying yang diao kong ye you chong yao jie jian yi yi 。
论文参考文献
论文详细介绍
论文作者分别是来自中国农业科学院的刘邦民，发表于刊物中国农业科学院2019-07-05论文，是一篇关于腐胺论文,断奶仔猪论文,小肠黏膜上皮细胞论文,黏膜损伤论文,炎症反应论文,中国农业科学院2019-07-05论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自中国农业科学院2019-07-05论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：腐胺论文; 断奶仔猪论文; 小肠黏膜上皮细胞论文; 黏膜损伤论文; 炎症反应论文; 中国农业科学院2019-07-05论文;