本文主要研究内容
作者潘绿昌（2019）在《贵州不同生态区灰毡毛忍冬和黄褐毛忍冬生物学特性、品质及产量的研究》一文中研究指出：灰毡/黄褐毛忍冬是贵州规模化种植的主要山银花品种,近年来,由于山银花价格低迷,经济效益欠佳,质量和产量参差不齐,品种混杂,药农积极性不高,导致贵州山银花种植面积持续减少。由于对山银花生物学特性、化学成分及药理药效等方面认识不足,系统研究不够,盲目扩大种植区域致使山银花产、质量下降,无能力抵御市场风险。因此,系统研究山银花生物学特性、光合特性、品质及产量迫在眉睫。本试验为揭示贵州不同生态区灰毡毛忍冬和黄褐毛忍冬的生物学特性及其差异、品质及产量情况,首先对采集于贵州各地的灰毡毛忍冬和黄褐毛忍冬两个物种进行DNA条形码鉴定,其次以贵州黔北绥阳和黔中安顺种植的灰毡毛忍冬、黔西南安龙和黔中清镇种植的黄褐毛忍冬为材料,系统研究其在各生态点的生物学特性、光合特性、品质及产量表现情况。主要研究结果如下:1.DNA条形码鉴定研究表明,灰毡毛忍冬和黄褐毛忍冬ITS2共有序列长度分别为404 bp、395 bp,相似度达99.49%;psbA-trnH共有序列长度分别为409 bp、410 bp,相似度达97.52%,黄褐毛忍冬psbA-trnH共有序列在5′端多2个碱基,3′末端多5个碱基,中间部分少6个碱基以及10个碱基位点上与灰毡毛忍冬不一样。psbA-trnH序列可鉴定区分灰毡毛忍冬和黄褐毛忍冬,ITS2序列无法准确鉴定两者。将psbA-trnH序列作为鉴定灰毡毛忍冬和黄褐毛忍冬的DNA条形码,推荐ITS2序列作为psbA-trnH序列的辅助序列加以鉴定。2.黄褐毛忍冬安龙和清镇试验点土壤肥力均属肥沃,土壤综合肥力系数（P综）分别为2.34、2.20;灰毡毛忍冬安顺试验点土壤肥力属肥沃,绥阳试验点土壤肥力属一般,安顺和绥阳试验点土壤综合肥力系数（P综）分别为1.90、1.54。总体看来,黄褐毛忍冬安龙试验点土壤肥力高于清镇试验点,灰毡毛忍冬安顺试验点土壤肥力高于绥阳试验点。黄褐毛忍冬安龙试验点多项气象要素值均高于清镇试验点,灰毡毛忍冬绥阳试验点气象要素值与安顺试验点差异不大。3.灰毡毛忍冬营养枝条生长量在绥阳生态点远高于安顺生态点,花枝生长量无显著差异,黄褐毛忍冬营养枝生长量在安龙生态点高于清镇生态点,花枝生长量变化并无显著差异;贵州灰毡毛忍冬物候期划分为新梢萌发期、营养枝生长期、越冬期、花枝萌发期、花枝生长期、显蕾期和开花期;黄褐毛忍冬物候期划分为新梢萌发期、营养枝生长期、缓慢生长期、花枝萌发期、花枝生长期、显蕾期和开花期。贵州灰毡毛忍冬花发育时期分为米粒期、三青期、青白期、二白期、大白期、银花期和金花期七个时期;黄褐毛忍冬花发育时期分为米粒期、青色期、青白期、大白期、银花期和金花期六个时期。灰毡毛忍冬病虫害主要有白粉病、根腐病和胡萝卜微管蚜,对植株生长和产量有明显影响,且绥阳生态点较安顺生态点更严重,黄褐毛忍冬无明显病虫害。4.灰毡/黄褐毛忍冬净光合速率、气孔导度日变化均为双峰曲线,蒸腾速率呈单峰曲线,胞间CO2浓度早晨和傍晚时段高于中午和下午时段,水分利用效率上午时段高于下午时段。灰毡毛忍冬绥阳生态点光合能力强于安顺生态点,黄褐毛忍冬清镇生态点净光合速率最大,光合能力强于安龙生态点,安龙生态点利用弱光能力强,安龙生态点光合作用受到Rubisco催化的羧化作用最大催化速率、电子传递速率控制的RuBP最大再生速率以及由磷酸丙糖运输速率控制的RuBP再生的限制作用较大。5.灰毡毛忍冬绥阳生态点花蕾发育优于安顺生态点,黄褐毛忍冬花蕾发育在安龙和清镇两生态点无较大差异。灰毡毛忍冬和黄褐毛忍冬花蕾大白期品质高于花蕾银花期,绥阳生态点的灰毡毛忍冬品质高于安顺生态点,黄褐毛忍冬品质在安龙和清镇两生态点无较大差异。灰毡毛忍冬绥阳生态点亩产量为334.13kg,单株产量为4.22kg,安顺生态点亩产量为212.72kg,单株产量为2.25kg,绥阳生态点单株产量和亩产量均高于安顺生态点;黄褐毛忍冬安龙生态点亩产量为244.37kg,单株产量为2.12kg,清镇生态点亩产量为228.58kg,单株产量为2.02kg,安龙生态点单株产量和亩产量均高于清镇生态点。综上所述,灰毡毛忍冬在黔北生态点种植较黔中生态点更好,黄褐毛忍冬对环境适应能力较强,在黔西南和黔中生态点均能适应。
Abstract
hui zhan /huang he mao ren dong shi gui zhou gui mo hua chong zhi de zhu yao shan yin hua pin chong ,jin nian lai ,you yu shan yin hua jia ge di mi ,jing ji xiao yi qian jia ,zhi liang he chan liang can cha bu ji ,pin chong hun za ,yao nong ji ji xing bu gao ,dao zhi gui zhou shan yin hua chong zhi mian ji chi xu jian shao 。you yu dui shan yin hua sheng wu xue te xing 、hua xue cheng fen ji yao li yao xiao deng fang mian ren shi bu zu ,ji tong yan jiu bu gou ,mang mu kuo da chong zhi ou yu zhi shi shan yin hua chan 、zhi liang xia jiang ,mo neng li di yu shi chang feng xian 。yin ci ,ji tong yan jiu shan yin hua sheng wu xue te xing 、guang ge te xing 、pin zhi ji chan liang pai zai mei jie 。ben shi yan wei jie shi gui zhou bu tong sheng tai ou hui zhan mao ren dong he huang he mao ren dong de sheng wu xue te xing ji ji cha yi 、pin zhi ji chan liang qing kuang ,shou xian dui cai ji yu gui zhou ge de de hui zhan mao ren dong he huang he mao ren dong liang ge wu chong jin hang DNAtiao xing ma jian ding ,ji ci yi gui zhou qian bei sui yang he qian zhong an shun chong zhi de hui zhan mao ren dong 、qian xi na an long he qian zhong qing zhen chong zhi de huang he mao ren dong wei cai liao ,ji tong yan jiu ji zai ge sheng tai dian de sheng wu xue te xing 、guang ge te xing 、pin zhi ji chan liang biao xian qing kuang 。zhu yao yan jiu jie guo ru xia :1.DNAtiao xing ma jian ding yan jiu biao ming ,hui zhan mao ren dong he huang he mao ren dong ITS2gong you xu lie chang du fen bie wei 404 bp、395 bp,xiang shi du da 99.49%;psbA-trnHgong you xu lie chang du fen bie wei 409 bp、410 bp,xiang shi du da 97.52%,huang he mao ren dong psbA-trnHgong you xu lie zai 5′duan duo 2ge jian ji ,3′mo duan duo 5ge jian ji ,zhong jian bu fen shao 6ge jian ji yi ji 10ge jian ji wei dian shang yu hui zhan mao ren dong bu yi yang 。psbA-trnHxu lie ke jian ding ou fen hui zhan mao ren dong he huang he mao ren dong ,ITS2xu lie mo fa zhun que jian ding liang zhe 。jiang psbA-trnHxu lie zuo wei jian ding hui zhan mao ren dong he huang he mao ren dong de DNAtiao xing ma ,tui jian ITS2xu lie zuo wei psbA-trnHxu lie de fu zhu xu lie jia yi jian ding 。2.huang he mao ren dong an long he qing zhen shi yan dian tu rang fei li jun shu fei wo ,tu rang zeng ge fei li ji shu （Pzeng ）fen bie wei 2.34、2.20;hui zhan mao ren dong an shun shi yan dian tu rang fei li shu fei wo ,sui yang shi yan dian tu rang fei li shu yi ban ,an shun he sui yang shi yan dian tu rang zeng ge fei li ji shu （Pzeng ）fen bie wei 1.90、1.54。zong ti kan lai ,huang he mao ren dong an long shi yan dian tu rang fei li gao yu qing zhen shi yan dian ,hui zhan mao ren dong an shun shi yan dian tu rang fei li gao yu sui yang shi yan dian 。huang he mao ren dong an long shi yan dian duo xiang qi xiang yao su zhi jun gao yu qing zhen shi yan dian ,hui zhan mao ren dong sui yang shi yan dian qi xiang yao su zhi yu an shun shi yan dian cha yi bu da 。3.hui zhan mao ren dong ying yang zhi tiao sheng chang liang zai sui yang sheng tai dian yuan gao yu an shun sheng tai dian ,hua zhi sheng chang liang mo xian zhe cha yi ,huang he mao ren dong ying yang zhi sheng chang liang zai an long sheng tai dian gao yu qing zhen sheng tai dian ,hua zhi sheng chang liang bian hua bing mo xian zhe cha yi ;gui zhou hui zhan mao ren dong wu hou ji hua fen wei xin sao meng fa ji 、ying yang zhi sheng chang ji 、yue dong ji 、hua zhi meng fa ji 、hua zhi sheng chang ji 、xian lei ji he kai hua ji ;huang he mao ren dong wu hou ji hua fen wei xin sao meng fa ji 、ying yang zhi sheng chang ji 、huan man sheng chang ji 、hua zhi meng fa ji 、hua zhi sheng chang ji 、xian lei ji he kai hua ji 。gui zhou hui zhan mao ren dong hua fa yo shi ji fen wei mi li ji 、san qing ji 、qing bai ji 、er bai ji 、da bai ji 、yin hua ji he jin hua ji qi ge shi ji ;huang he mao ren dong hua fa yo shi ji fen wei mi li ji 、qing se ji 、qing bai ji 、da bai ji 、yin hua ji he jin hua ji liu ge shi ji 。hui zhan mao ren dong bing chong hai zhu yao you bai fen bing 、gen fu bing he hu luo bo wei guan ya ,dui zhi zhu sheng chang he chan liang you ming xian ying xiang ,ju sui yang sheng tai dian jiao an shun sheng tai dian geng yan chong ,huang he mao ren dong mo ming xian bing chong hai 。4.hui zhan /huang he mao ren dong jing guang ge su lv 、qi kong dao du ri bian hua jun wei shuang feng qu xian ,zheng teng su lv cheng chan feng qu xian ,bao jian CO2nong du zao chen he bang wan shi duan gao yu zhong wu he xia wu shi duan ,shui fen li yong xiao lv shang wu shi duan gao yu xia wu shi duan 。hui zhan mao ren dong sui yang sheng tai dian guang ge neng li jiang yu an shun sheng tai dian ,huang he mao ren dong qing zhen sheng tai dian jing guang ge su lv zui da ,guang ge neng li jiang yu an long sheng tai dian ,an long sheng tai dian li yong ruo guang neng li jiang ,an long sheng tai dian guang ge zuo yong shou dao Rubiscocui hua de suo hua zuo yong zui da cui hua su lv 、dian zi chuan di su lv kong zhi de RuBPzui da zai sheng su lv yi ji you lin suan bing tang yun shu su lv kong zhi de RuBPzai sheng de xian zhi zuo yong jiao da 。5.hui zhan mao ren dong sui yang sheng tai dian hua lei fa yo you yu an shun sheng tai dian ,huang he mao ren dong hua lei fa yo zai an long he qing zhen liang sheng tai dian mo jiao da cha yi 。hui zhan mao ren dong he huang he mao ren dong hua lei da bai ji pin zhi gao yu hua lei yin hua ji ,sui yang sheng tai dian de hui zhan mao ren dong pin zhi gao yu an shun sheng tai dian ,huang he mao ren dong pin zhi zai an long he qing zhen liang sheng tai dian mo jiao da cha yi 。hui zhan mao ren dong sui yang sheng tai dian mu chan liang wei 334.13kg,chan zhu chan liang wei 4.22kg,an shun sheng tai dian mu chan liang wei 212.72kg,chan zhu chan liang wei 2.25kg,sui yang sheng tai dian chan zhu chan liang he mu chan liang jun gao yu an shun sheng tai dian ;huang he mao ren dong an long sheng tai dian mu chan liang wei 244.37kg,chan zhu chan liang wei 2.12kg,qing zhen sheng tai dian mu chan liang wei 228.58kg,chan zhu chan liang wei 2.02kg,an long sheng tai dian chan zhu chan liang he mu chan liang jun gao yu qing zhen sheng tai dian 。zeng shang suo shu ,hui zhan mao ren dong zai qian bei sheng tai dian chong zhi jiao qian zhong sheng tai dian geng hao ,huang he mao ren dong dui huan jing kuo ying neng li jiao jiang ,zai qian xi na he qian zhong sheng tai dian jun neng kuo ying 。
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论文作者分别是来自贵州大学的潘绿昌，发表于刊物贵州大学2019-07-16论文，是一篇关于不同生态点论文,灰毡毛忍冬论文,黄褐毛忍冬论文,生物学特性论文,光合特性论文,产量及品质论文,贵州大学2019-07-16论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自贵州大学2019-07-16论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：不同生态点论文; 灰毡毛忍冬论文; 黄褐毛忍冬论文; 生物学特性论文; 光合特性论文; 产量及品质论文; 贵州大学2019-07-16论文;