本文主要研究内容
作者（2019）在《Novel Graphene Biosensor Based on the Functionalization of Multifunctional Nano-bovine Serum Albumin for the Highly Sensitive Detection of Cancer Biomarkers》一文中研究指出：A simple,convenient,and highly sensitive bio-interface for graphene field-effect transistors（GFETs） based on multifunctional nano-denatured bovine serum albumin（nano-dBSA） functionalization was developed to target cancer bio-markers.The novel graphene–protein bioelectronic interface was constructed by heating to denature native BSA on the graphene substrate surface.The formed nano-d BSA film served as the cross-linker to immobilize monoclonal antibody against car-cinoembryonic antigen（anti-CEA mAb） on the graphene channel activated by EDC and Sulfo-NHS.The nano-dBSA film worked as a self-protecting layer of graphene to prevent surface contamination by lithographic processing.The improved GFETbiosensor exhibited good specificity and high sensitivity toward the target at an ultralow concentration of 337.58 fg mL-1.The electrical detection of the binding of CEA followed the Hill model for ligand–receptor interaction,indicating the negative binding cooperativity between CEA and anti-CEA mAb with a dissociation constant of 6.82×10-10M.The multifunctional nano-dBSA functionalization can confer a new function to graphene-like 2D nanomaterials and provide a promising bio-functionalization method for clinical application in biosensing,nanomedicine,and drug delivery.
Abstract
A simple,convenient,and highly sensitive bio-interface for graphene field-effect transistors（GFETs） based on multifunctional nano-denatured bovine serum albumin（nano-dBSA） functionalization was developed to target cancer bio-markers.The novel graphene–protein bioelectronic interface was constructed by heating to denature native BSA on the graphene substrate surface.The formed nano-d BSA film served as the cross-linker to immobilize monoclonal antibody against car-cinoembryonic antigen（anti-CEA mAb） on the graphene channel activated by EDC and Sulfo-NHS.The nano-dBSA film worked as a self-protecting layer of graphene to prevent surface contamination by lithographic processing.The improved GFETbiosensor exhibited good specificity and high sensitivity toward the target at an ultralow concentration of 337.58 fg mL-1.The electrical detection of the binding of CEA followed the Hill model for ligand–receptor interaction,indicating the negative binding cooperativity between CEA and anti-CEA mAb with a dissociation constant of 6.82×10-10M.The multifunctional nano-dBSA functionalization can confer a new function to graphene-like 2D nanomaterials and provide a promising bio-functionalization method for clinical application in biosensing,nanomedicine,and drug delivery.
论文参考文献
[1].Polarographic catalytic wave of hydrogen——Parallel catalytic hydrogen wave of bovine serum albumin in the presence of oxidants[J]. 过玮,宋俊峰,刘利民,林洪. Science in China(Series B,Chemistry).2002(02)[2].Studies on Reaction Mechanism Between Sparfloxacin and Bovine Serum Albumin[J]. GUO Ming **, ZOU Jian-wei, YI Ping-gui, SHANG Zhi-cai, HU Gui-xiang and YU Qing-sen *** Department of Chemistry, Zhejiang University, Hangzhou 310027, P. R. China. Chemical Research in Chinese Universities.2004(01)[3].New Sorbent for Bilirubin Removal from Human Plasma: Albumin Immobilized Microporous Membranous PTFE Capillaries[J]. Lei ZHANG, Gu JIN* Department of Chemistry, University of Science and Technology of China, Hefei 230026. Chinese Chemical Letters.2005(11)[4].Synthesis of Metal Porphyrins Tailed with Salicylic Acid and their Interaction with Bovine Serum Albumin[J]. Tao JIA1, Kai WANG1,2, Yi Mei ZHAO2,Zao Ying LI1* 1 Department of Chemistry, Wuhan University, Wuhan 430072 2 Institute of RHK Co., Ltd., Wuhan 430070. Chinese Chemical Letters.2004(03)[5].Characterization of the Interaction between Bovine Serum Albumin and Lomefloxacin by Capillary Zone Electrophoresis[J]. Ming GUO1, Qing Sen YU1*, Jian Wei YAN2, Fei TAN1, Guo Zheng MA1 1 Department of Chemistry, Zhejiang University, Hangzhou 3100271 2 Department of Chinese Medicine Science & Engineering, Zhejiang University, Hangzhou 3100271. Chinese Chemical Letters.2004(11)[6].Human Serum Albumin Hybrid Incorporating Synthetic Hemes:A Novel O2-Carrying Hemoprotein[J]. Eishun TSUCHIDA *, Teruyuki KOMATSU, Yuping WU, Yubin HUANG(Department of Polymer Chemistry,Advanced Research Institute for Science and Engineering,Waseda University,Tokyo 169-8555,Japan) Dedicated to the Occasion of the 80th Birthday of Prof.HUANG. 应用化学.2001(05)[7].Voltammetric Studies of the Interaction of Tris (1,10-phenanthroline) Cobalt (III) with Bovine Serum Albumin[J]. Li Ping LU, Bao Kang JIN* Department of chemistry, Anhui University, Hefei 230039. Chinese Chemical Letters.2001(11)[8].Study on the Interaction between Strychnine and Bovine Serum Albumin by Capillary Electrophoretic Frontal Analysis[J]. Shao Ping FU, Xiao Zhe ZHANG, Feng ZHANG, Qing XU, Hong Bin XIAO, Xin Miao LIANG Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023. Chinese Chemical Letters.2005(09)[9].Sonocatalytic Damage of Bovine Serum Albumin （BSA） in the Presence of Nanometer Titanium Dioxide （TiO2） Catalyst[J]. Jun WANG*, Jing WU, Zhao Hong ZHANG, Xiang Dong ZHANG, Lei WANG, Liang XU, Bao Dong GU O, Hong LI, Jian TONG Department of Chemistry, Liaoning University, Shenyang 110036. Chinese Chemical Letters.2005(08)[10].Determination of Enantiomeric Compositions of Tryptophan by Chemometric Analysis of the Fluorescence Spectra of Bovine Serum Albumin Receptor-ligand Mixtures[J]. Yun Xia WANG1, 2, Fang ZHANG3, Jing LIANG3, Hua LI3, Ji Lie KONG1? 1 Department of Chemistry, Fudan University, Shanghai 200433 2 Department of Chemistry, Northwest University, Xi’an 710069 3 Institute of Analytical Science, Northwest University, Xi’an 710069. Chinese Chemical Letters.2006(12)
论文详细介绍
论文作者分别是来自Nano-Micro Letters的，发表于刊物Nano-Micro Letters2019年02期论文，是一篇关于,Nano-Micro Letters2019年02期论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自Nano-Micro Letters2019年02期论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：Nano-Micro Letters2019年02期论文;
：Novel Graphene Biosensor Based on the Functionalization of Multifunctional Nano-bovine Serum Albumin for the Highly Sensitive Detection of Cancer Biomarkers论文
下载Doc文档