本文主要研究内容
作者雷伟岩（2019）在《纳米铂(Ⅳ)-铁(Ⅲ)双金属配位聚合物用于肿瘤的化学和化学动力学联合治疗》一文中研究指出：化疗是应用最广泛的肿瘤治疗手段之一。作为一线化疗药物,顺铂对多种肿瘤都具有治疗效果,但因其存在副作用和耐药性等缺陷,严重限制了其临床应用。四价铂前药以其良好的稳定性、较好的脂溶性和较低的毒性等优势,克服了传统铂类药物的缺陷。然而,四价铂前药在肾脏中被快速消除,导致了有效治疗剂量的减少。除化疗外,其他的肿瘤治疗手段有光热治疗、光动力学治疗、化学动力学治疗和免疫治疗等。其中,化学动力学治疗利用铁基的纳米材料在肿瘤的微酸性条件下解离产生金属离子,通过芬顿或类芬顿反应,催化体内过量的过氧化氢分解,产生羟基自由基,导致细胞凋亡。将四价铂前药和化学动力学疗法结合起来,利用化学动力学疗法产生的羟基自由基和四价铂的还原产物二价铂共同杀伤肿瘤细胞,同时降低体内谷胱甘肽和过氧化氢的含量,可降低顺铂毒副作用和改善肿瘤微环境。为克服顺铂毒副作用大的缺点,同时增强化学动力学治疗效果,改善肿瘤微环境,以顺铂为原料,对苯二甲酸为轴向配体,利用氧化反应和亲核取代反应合成了配合物Pt（Ⅳ）-HBdc2,再以Pt（Ⅳ）-HBdc2和高氯酸铁为原料,通过溶剂热法合成了纳米铂（Ⅳ）-铁（Ⅲ）双金属配位聚合物Pt/Fe-Bdc。采用NMR、IR、MS等手段对Pt（Ⅳ）-HBdc2进行表征;采用SEM、TEM、XRD、BET、Raman和IR等手段对Pt/Fe-Bdc进行表征;采用分光光度法对Pt/Fe-Bdc在不同pH环境中的稳定性进行测定;采用MTT法测试了Pt（Ⅳ）-HBdc2和Pt/Fe-Bdc对细胞活力的影响,采用流式细胞分析法测试Pt/Fe-Bdc诱导肿瘤细胞凋亡的情况;采用亚甲基蓝降解法和共聚焦荧光成像法对Pt/Fe-Bdc产生活性氧能力进行了测定;通过分光光度法探究了Pt（Ⅳ）-HBdc2和Pt/Fe-Bdc处理后细胞内谷胱甘肽的水平;使用nu/nu nude小鼠建立了皮下荷瘤小鼠模型,对Pt/Fe-Bdc的肿瘤抑制率进行研究。结果表明:Pt/Fe-Bdc为90 nm左右的正八面体纳米晶体,其中Pt（Ⅳ）-HBdc2和Fe3+离子以1:1的比例配位;Pt/Fe-Bdc在弱酸性（pH=5.4）条件下可逐渐解离,释放出Fe3+离子和Pt（Ⅳ）-HBdc2,其中Fe3+离子可以在肿瘤组织发生类芬顿反应产生羟基自由基杀伤肿瘤细胞,Pt（Ⅳ）-HBdc2在肿瘤细胞中被谷胱甘肽还原成顺铂,杀伤肿瘤细胞。Pt（Ⅳ）-HBdc2和Pt/Fe-Bdc只对肿瘤细胞有较为明显的杀伤效果,对正常细胞几乎没有影响;Pt/Fe-Bdc在肿瘤细胞中催化过氧化氢产生羟基自由基,并降低细胞内谷胱甘肽含量;Pt/Fe-Bdc可以有效地抑制小鼠肿瘤生长,和顺铂相比毒副作用较低,抑瘤效果更好。Pt/Fe-Bdc结合化学治疗和化学动力学治疗,有较好的体内外抗肿瘤效果,此外,消耗了细胞内的谷胱甘肽和过氧化氢,可有效改善肿瘤微环境。Pt/Fe-Bdc具有对正常组织毒副作用低和抗肿瘤活性强等优势,为实现肿瘤的有效治疗提供了新思路。
Abstract
hua liao shi ying yong zui an fan de zhong liu zhi liao shou duan zhi yi 。zuo wei yi xian hua liao yao wu ,shun bo dui duo chong zhong liu dou ju you zhi liao xiao guo ,dan yin ji cun zai fu zuo yong he nai yao xing deng que xian ,yan chong xian zhi le ji lin chuang ying yong 。si jia bo qian yao yi ji liang hao de wen ding xing 、jiao hao de zhi rong xing he jiao di de du xing deng you shi ,ke fu le chuan tong bo lei yao wu de que xian 。ran er ,si jia bo qian yao zai shen zang zhong bei kuai su xiao chu ,dao zhi le you xiao zhi liao ji liang de jian shao 。chu hua liao wai ,ji ta de zhong liu zhi liao shou duan you guang re zhi liao 、guang dong li xue zhi liao 、hua xue dong li xue zhi liao he mian yi zhi liao deng 。ji zhong ,hua xue dong li xue zhi liao li yong tie ji de na mi cai liao zai zhong liu de wei suan xing tiao jian xia jie li chan sheng jin shu li zi ,tong guo fen du huo lei fen du fan ying ,cui hua ti nei guo liang de guo yang hua qing fen jie ,chan sheng qiang ji zi you ji ,dao zhi xi bao diao wang 。jiang si jia bo qian yao he hua xue dong li xue liao fa jie ge qi lai ,li yong hua xue dong li xue liao fa chan sheng de qiang ji zi you ji he si jia bo de hai yuan chan wu er jia bo gong tong sha shang zhong liu xi bao ,tong shi jiang di ti nei gu guang gan tai he guo yang hua qing de han liang ,ke jiang di shun bo du fu zuo yong he gai shan zhong liu wei huan jing 。wei ke fu shun bo du fu zuo yong da de que dian ,tong shi zeng jiang hua xue dong li xue zhi liao xiao guo ,gai shan zhong liu wei huan jing ,yi shun bo wei yuan liao ,dui ben er jia suan wei zhou xiang pei ti ,li yong yang hua fan ying he qin he qu dai fan ying ge cheng le pei ge wu Pt（Ⅳ）-HBdc2,zai yi Pt（Ⅳ）-HBdc2he gao lv suan tie wei yuan liao ,tong guo rong ji re fa ge cheng le na mi bo （Ⅳ）-tie （Ⅲ）shuang jin shu pei wei ju ge wu Pt/Fe-Bdc。cai yong NMR、IR、MSdeng shou duan dui Pt（Ⅳ）-HBdc2jin hang biao zheng ;cai yong SEM、TEM、XRD、BET、Ramanhe IRdeng shou duan dui Pt/Fe-Bdcjin hang biao zheng ;cai yong fen guang guang du fa dui Pt/Fe-Bdczai bu tong pHhuan jing zhong de wen ding xing jin hang ce ding ;cai yong MTTfa ce shi le Pt（Ⅳ）-HBdc2he Pt/Fe-Bdcdui xi bao huo li de ying xiang ,cai yong liu shi xi bao fen xi fa ce shi Pt/Fe-Bdcyou dao zhong liu xi bao diao wang de qing kuang ;cai yong ya jia ji lan jiang jie fa he gong ju jiao ying guang cheng xiang fa dui Pt/Fe-Bdcchan sheng huo xing yang neng li jin hang le ce ding ;tong guo fen guang guang du fa tan jiu le Pt（Ⅳ）-HBdc2he Pt/Fe-Bdcchu li hou xi bao nei gu guang gan tai de shui ping ;shi yong nu/nu nudexiao shu jian li le pi xia he liu xiao shu mo xing ,dui Pt/Fe-Bdcde zhong liu yi zhi lv jin hang yan jiu 。jie guo biao ming :Pt/Fe-Bdcwei 90 nmzuo you de zheng ba mian ti na mi jing ti ,ji zhong Pt（Ⅳ）-HBdc2he Fe3+li zi yi 1:1de bi li pei wei ;Pt/Fe-Bdczai ruo suan xing （pH=5.4）tiao jian xia ke zhu jian jie li ,shi fang chu Fe3+li zi he Pt（Ⅳ）-HBdc2,ji zhong Fe3+li zi ke yi zai zhong liu zu zhi fa sheng lei fen du fan ying chan sheng qiang ji zi you ji sha shang zhong liu xi bao ,Pt（Ⅳ）-HBdc2zai zhong liu xi bao zhong bei gu guang gan tai hai yuan cheng shun bo ,sha shang zhong liu xi bao 。Pt（Ⅳ）-HBdc2he Pt/Fe-Bdczhi dui zhong liu xi bao you jiao wei ming xian de sha shang xiao guo ,dui zheng chang xi bao ji hu mei you ying xiang ;Pt/Fe-Bdczai zhong liu xi bao zhong cui hua guo yang hua qing chan sheng qiang ji zi you ji ,bing jiang di xi bao nei gu guang gan tai han liang ;Pt/Fe-Bdcke yi you xiao de yi zhi xiao shu zhong liu sheng chang ,he shun bo xiang bi du fu zuo yong jiao di ,yi liu xiao guo geng hao 。Pt/Fe-Bdcjie ge hua xue zhi liao he hua xue dong li xue zhi liao ,you jiao hao de ti nei wai kang zhong liu xiao guo ,ci wai ,xiao hao le xi bao nei de gu guang gan tai he guo yang hua qing ,ke you xiao gai shan zhong liu wei huan jing 。Pt/Fe-Bdcju you dui zheng chang zu zhi du fu zuo yong di he kang zhong liu huo xing jiang deng you shi ,wei shi xian zhong liu de you xiao zhi liao di gong le xin sai lu 。
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论文作者分别是来自河北大学的雷伟岩，发表于刊物河北大学2019-07-25论文，是一篇关于化学动力学治疗论文,联合治疗论文,肿瘤微环境论文,铂类抗肿瘤药物论文,河北大学2019-07-25论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自河北大学2019-07-25论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：化学动力学治疗论文; 联合治疗论文; 肿瘤微环境论文; 铂类抗肿瘤药物论文; 河北大学2019-07-25论文;