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作者唐少军,陈定安,邵晨霞,许隽,杨祎,靳磊,吴胜莲（2019）在《一株野生粗毛纤孔菌的分类鉴定及其发酵上清液的抗肿瘤活性》一文中研究指出：【目的】对一株野生粗毛纤孔菌进行分类学鉴定,并分析其发酵上清液抗肿瘤活性,为粗毛纤孔菌的开发利用及抗肿瘤活性机制研究提供理论依据。【方法】通过形态特性观察、ITS序列分析及系统发育进化树构建等方法对一株野生粗毛纤孔菌(简称IH3菌株)进行分类学鉴定,并利用B16、Hep-3B、Hela、MCF-7、HCT-116和4T1 6种肿瘤细胞及正常细胞HUVEC分析该菌株发酵上清液的抗肿瘤谱。从石油醚、乙酸乙酯和正丁醇中筛选出萃取抗肿瘤活性物质的最佳有机溶剂,利用液相色谱分离技术分离抗肿瘤活性组分,并利用MTT(四唑盐)法分析活性组分对不同肿瘤细胞的半致死浓度(IC50)。【结果】IH3菌株的形态特性与粗毛纤孔菌相似,且其ITS序列与粗毛纤孔菌(MF183947.1)的相似度最高,为98%,鉴定为粗毛纤孔菌。系统发育进化树分析结果也显示,IH3菌株属于纤层孔菌属类群,与3个粗毛纤孔菌菌株的亲缘关系较近。IH3菌株的发酵上清液对不同肿瘤细胞的抑制活性排序为:B16>Hep-3B>Hela>MCF-7>4T1=HCT-116。正丁醇萃取的IH3菌株发酵上清液抗肿瘤活性组分对B16肿瘤细胞的抑制率最高,达80%,说明正丁醇对发酵上清液中抗肿瘤活性组分的萃取效果最佳。利用液相色谱分离方法可有效获得纯度较高的单一抗肿瘤活性组分(WIH3),该活性组分对B16、Hep-3B、Hela和MCF-7肿瘤细胞的半致死浓度(IC50)分别为29.322、37.387、47.029和58.009μg/mL。【结论】IH3菌株被鉴定为粗毛纤孔菌,与已发表的粗毛纤孔菌菌株存在遗传差异,可能为粗毛纤孔菌的新亚种,其发酵上清液具有广谱的抗肿瘤活性。
Abstract
【mu de 】dui yi zhu ye sheng cu mao qian kong jun jin hang fen lei xue jian ding ,bing fen xi ji fa jiao shang qing ye kang zhong liu huo xing ,wei cu mao qian kong jun de kai fa li yong ji kang zhong liu huo xing ji zhi yan jiu di gong li lun yi ju 。【fang fa 】tong guo xing tai te xing guan cha 、ITSxu lie fen xi ji ji tong fa yo jin hua shu gou jian deng fang fa dui yi zhu ye sheng cu mao qian kong jun (jian chen IH3jun zhu )jin hang fen lei xue jian ding ,bing li yong B16、Hep-3B、Hela、MCF-7、HCT-116he 4T1 6chong zhong liu xi bao ji zheng chang xi bao HUVECfen xi gai jun zhu fa jiao shang qing ye de kang zhong liu pu 。cong dan you mi 、yi suan yi zhi he zheng ding chun zhong shai shua chu cui qu kang zhong liu huo xing wu zhi de zui jia you ji rong ji ,li yong ye xiang se pu fen li ji shu fen li kang zhong liu huo xing zu fen ,bing li yong MTT(si zuo yan )fa fen xi huo xing zu fen dui bu tong zhong liu xi bao de ban zhi si nong du (IC50)。【jie guo 】IH3jun zhu de xing tai te xing yu cu mao qian kong jun xiang shi ,ju ji ITSxu lie yu cu mao qian kong jun (MF183947.1)de xiang shi du zui gao ,wei 98%,jian ding wei cu mao qian kong jun 。ji tong fa yo jin hua shu fen xi jie guo ye xian shi ,IH3jun zhu shu yu qian ceng kong jun shu lei qun ,yu 3ge cu mao qian kong jun jun zhu de qin yuan guan ji jiao jin 。IH3jun zhu de fa jiao shang qing ye dui bu tong zhong liu xi bao de yi zhi huo xing pai xu wei :B16>Hep-3B>Hela>MCF-7>4T1=HCT-116。zheng ding chun cui qu de IH3jun zhu fa jiao shang qing ye kang zhong liu huo xing zu fen dui B16zhong liu xi bao de yi zhi lv zui gao ,da 80%,shui ming zheng ding chun dui fa jiao shang qing ye zhong kang zhong liu huo xing zu fen de cui qu xiao guo zui jia 。li yong ye xiang se pu fen li fang fa ke you xiao huo de chun du jiao gao de chan yi kang zhong liu huo xing zu fen (WIH3),gai huo xing zu fen dui B16、Hep-3B、Helahe MCF-7zhong liu xi bao de ban zhi si nong du (IC50)fen bie wei 29.322、37.387、47.029he 58.009μg/mL。【jie lun 】IH3jun zhu bei jian ding wei cu mao qian kong jun ,yu yi fa biao de cu mao qian kong jun jun zhu cun zai wei chuan cha yi ,ke neng wei cu mao qian kong jun de xin ya chong ,ji fa jiao shang qing ye ju you an pu de kang zhong liu huo xing 。
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论文作者分别是来自南方农业学报的唐少军,陈定安,邵晨霞,许隽,杨祎,靳磊,吴胜莲，发表于刊物南方农业学报2019年08期论文，是一篇关于粗毛纤孔菌论文,鉴定论文,抗肿瘤活性论文,液相色谱分离论文,半致死浓度论文,南方农业学报2019年08期论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自南方农业学报2019年08期论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：粗毛纤孔菌论文; 鉴定论文; 抗肿瘤活性论文; 液相色谱分离论文; 半致死浓度论文; 南方农业学报2019年08期论文;