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作者（2019）在《Involvement of sweet pepper Ca LOX2 in jasmonate-dependent induced defence against Western flower thrips》一文中研究指出：Insect herbivory can seriously hinder plant performance and reduce crop yield. Thrips are minute cell-content-feeding insects that are important vectors of viral plant pathogens, and are serious crop pests. We investigated the role of a sweet pepper(Capsicum annuum)lipoxygenase gene, Ca LOX2, in the defense of pepper plants against Western flower thrips(Frankliniella occidentalis).This was done through a combination of in-silico, transcriptional, behavioral and chemical analyses. Our data show that Ca LOX2 is involved in jasmonic acid(JA) biosynthesis and mediates plant resistance. Expression of the JA-related marker genes, Ca LOX2 and Ca PIN II, was induced by thrips feeding. Silencing of Ca LOX2 in pepper plants through virusinduced gene silencing(VIGS) resulted in low levels of Ca LOX2 transcripts, as well as significant reduction in theaccumulation of JA, and its derivatives, upon thrips feeding compared to control plants. Ca LOX2-silenced pepper plants exhibited enhanced susceptibility to thrips. This indicates that Ca LOX2 mediates JA-dependent signaling, resulting in defense against thrips. Furthermore, exogenous application of JA to pepper plants increased plant resistance to thrips,constrained thrips population development and made plants less attractive to thrips. Thus, a multidisciplinary approach shows that an intact lipoxygenase pathway mediates various components of sweet pepper defense against F. occidentalis.
Abstract
Insect herbivory can seriously hinder plant performance and reduce crop yield. Thrips are minute cell-content-feeding insects that are important vectors of viral plant pathogens, and are serious crop pests. We investigated the role of a sweet pepper(Capsicum annuum)lipoxygenase gene, Ca LOX2, in the defense of pepper plants against Western flower thrips(Frankliniella occidentalis).This was done through a combination of in-silico, transcriptional, behavioral and chemical analyses. Our data show that Ca LOX2 is involved in jasmonic acid(JA) biosynthesis and mediates plant resistance. Expression of the JA-related marker genes, Ca LOX2 and Ca PIN II, was induced by thrips feeding. Silencing of Ca LOX2 in pepper plants through virusinduced gene silencing(VIGS) resulted in low levels of Ca LOX2 transcripts, as well as significant reduction in theaccumulation of JA, and its derivatives, upon thrips feeding compared to control plants. Ca LOX2-silenced pepper plants exhibited enhanced susceptibility to thrips. This indicates that Ca LOX2 mediates JA-dependent signaling, resulting in defense against thrips. Furthermore, exogenous application of JA to pepper plants increased plant resistance to thrips,constrained thrips population development and made plants less attractive to thrips. Thus, a multidisciplinary approach shows that an intact lipoxygenase pathway mediates various components of sweet pepper defense against F. occidentalis.
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