本文主要研究内容
作者赵晓晓（2019）在《柳枝稷幼穗分化分期与TIFY基因家族鉴定》一文中研究指出：柳枝稷(Panicum virgatum L.)为禾本科多年生C4草本植物,近年来国际上已将其作为模式能源作物进行深入研究,但对柳枝稷生殖生长特性的研究相对较少。幼穗分化是营养生长与生殖生长的转折点,正确判断幼穗分化关键期对作物的生长发育调控、栽培管理与育种研究意义重大,可目前国内外尚未开展柳枝稷幼穗分化过程分期的研究。生殖生长是个体发育的重要阶段,受外界坏境和内部因素的共同调控,TIFY基因家族作为转录因子或转录因子互作蛋白,参与调控植物的生长发育、激素应答及胁迫响应等过程,然而柳枝稷TIFY基因家族研究尚未见报道。本研究为探索柳枝稷幼穗分化规律及TIFY基因家族在柳枝稷生殖生长过程中的功能,根据柳枝稷幼穗分化各阶段组织形态显微观察结果将柳枝稷幼穗分化过程进行了分期,并记录了对应的大田植株形态以供判别分化进程;对柳枝稷基因组中的TIFY基因家族成员进行了鉴定,并分析了各成员的进化关系、结构组成及表达特性;选定水稻中两个与花序发育有关的JAZ亚家族成员进行了同源克隆,并对两者的分子特征及亚细胞定位进行了分析且构建了基因过表达载体。论文从形态观察与分子调控两方面对柳枝稷的花序发育进行了研究,为柳枝稷生殖生长特性的探索奠定基础。主要结果如下:1.柳枝稷幼穗分化是一个慢-快-慢的过程,可分为9个连续的时期:苞原基分化期、枝梗原基分化期、小穗分化期、小花原基分化期、雌雄蕊原基分化期、药隔形成期、花药四分期、花药伸长期、羽状柱头形成期;每个时期均具有与之对应的植株外观形态以供判别幼穗分化进程:苞原基分化期至药隔形成期,叶龄、叶龄余数及可见叶数可用来判断幼穗分化所处时期,花药四分期至羽状柱头形成期,花序与小穗的长度及形态可用来辨别幼穗分化进程;柳枝稷幼穗分化起始至分化完全历时约3个月,植株历经拔节、孕穗、抽穗而开花,花序从上到下依次开花,花期可达3-4周。2.柳枝稷中共有48个TIFY成员,分属于TIFY(1个)、ZML(7个)、JAZ(40个)3个亚家族;PviTIFY基因分布在除染色体3以外的各条染色体上,其中半数成员集中定位在染色体9;PviTIFY蛋白依据进化关系及结构特点可分为5组:JAZⅠ、JAZⅡ、JAZⅢ、TIFY、ZML,每组内亲缘关系较近的成员具有相似的基因结构及保守基序组成;PviTIFY启动子区存在多种胁迫响应元件与激素信号调控元件,经定量实验证实PviTIFY10成员在JA处理及盐与干旱胁迫下反应强烈;JAZ亚家族部分成员尤其是PviTIFY10b,在花序与根的发育过程中有较高表达。根据PviTIFY的表达特性推测其可能在柳枝稷激素信号调控、胁迫防御反应及生长发育过程等方面发挥功能。3.成功克隆OsJAZ1与OsTIFY10b的同源基因PviTIFY3a与PviTIFY10b;两个基因所编码蛋白的保守结构域组成、进化特点、理化性质及二级结构组成等分子特征相似;PviTIFY3a-GFP融合蛋白瞬时表达结果显示PviTIFY3a定位于细胞核与细胞膜,软件预测PviTIFY10b很可能定位于细胞质;两个基因的过表达载体均已成功构建。
Abstract
liu zhi ji (Panicum virgatum L.)wei he ben ke duo nian sheng C4cao ben zhi wu ,jin nian lai guo ji shang yi jiang ji zuo wei mo shi neng yuan zuo wu jin hang shen ru yan jiu ,dan dui liu zhi ji sheng shi sheng chang te xing de yan jiu xiang dui jiao shao 。you sui fen hua shi ying yang sheng chang yu sheng shi sheng chang de zhuai she dian ,zheng que pan duan you sui fen hua guan jian ji dui zuo wu de sheng chang fa yo diao kong 、zai pei guan li yu yo chong yan jiu yi yi chong da ,ke mu qian guo nei wai shang wei kai zhan liu zhi ji you sui fen hua guo cheng fen ji de yan jiu 。sheng shi sheng chang shi ge ti fa yo de chong yao jie duan ,shou wai jie huai jing he nei bu yin su de gong tong diao kong ,TIFYji yin jia zu zuo wei zhuai lu yin zi huo zhuai lu yin zi hu zuo dan bai ,can yu diao kong zhi wu de sheng chang fa yo 、ji su ying da ji xie pai xiang ying deng guo cheng ,ran er liu zhi ji TIFYji yin jia zu yan jiu shang wei jian bao dao 。ben yan jiu wei tan suo liu zhi ji you sui fen hua gui lv ji TIFYji yin jia zu zai liu zhi ji sheng shi sheng chang guo cheng zhong de gong neng ,gen ju liu zhi ji you sui fen hua ge jie duan zu zhi xing tai xian wei guan cha jie guo jiang liu zhi ji you sui fen hua guo cheng jin hang le fen ji ,bing ji lu le dui ying de da tian zhi zhu xing tai yi gong pan bie fen hua jin cheng ;dui liu zhi ji ji yin zu zhong de TIFYji yin jia zu cheng yuan jin hang le jian ding ,bing fen xi le ge cheng yuan de jin hua guan ji 、jie gou zu cheng ji biao da te xing ;shua ding shui dao zhong liang ge yu hua xu fa yo you guan de JAZya jia zu cheng yuan jin hang le tong yuan ke long ,bing dui liang zhe de fen zi te zheng ji ya xi bao ding wei jin hang le fen xi ju gou jian le ji yin guo biao da zai ti 。lun wen cong xing tai guan cha yu fen zi diao kong liang fang mian dui liu zhi ji de hua xu fa yo jin hang le yan jiu ,wei liu zhi ji sheng shi sheng chang te xing de tan suo dian ding ji chu 。zhu yao jie guo ru xia :1.liu zhi ji you sui fen hua shi yi ge man -kuai -man de guo cheng ,ke fen wei 9ge lian xu de shi ji :bao yuan ji fen hua ji 、zhi geng yuan ji fen hua ji 、xiao sui fen hua ji 、xiao hua yuan ji fen hua ji 、ci xiong rui yuan ji fen hua ji 、yao ge xing cheng ji 、hua yao si fen ji 、hua yao shen chang ji 、yu zhuang zhu tou xing cheng ji ;mei ge shi ji jun ju you yu zhi dui ying de zhi zhu wai guan xing tai yi gong pan bie you sui fen hua jin cheng :bao yuan ji fen hua ji zhi yao ge xing cheng ji ,xie ling 、xie ling yu shu ji ke jian xie shu ke yong lai pan duan you sui fen hua suo chu shi ji ,hua yao si fen ji zhi yu zhuang zhu tou xing cheng ji ,hua xu yu xiao sui de chang du ji xing tai ke yong lai bian bie you sui fen hua jin cheng ;liu zhi ji you sui fen hua qi shi zhi fen hua wan quan li shi yao 3ge yue ,zhi zhu li jing ba jie 、yun sui 、chou sui er kai hua ,hua xu cong shang dao xia yi ci kai hua ,hua ji ke da 3-4zhou 。2.liu zhi ji zhong gong you 48ge TIFYcheng yuan ,fen shu yu TIFY(1ge )、ZML(7ge )、JAZ(40ge )3ge ya jia zu ;PviTIFYji yin fen bu zai chu ran se ti 3yi wai de ge tiao ran se ti shang ,ji zhong ban shu cheng yuan ji zhong ding wei zai ran se ti 9;PviTIFYdan bai yi ju jin hua guan ji ji jie gou te dian ke fen wei 5zu :JAZⅠ、JAZⅡ、JAZⅢ、TIFY、ZML,mei zu nei qin yuan guan ji jiao jin de cheng yuan ju you xiang shi de ji yin jie gou ji bao shou ji xu zu cheng ;PviTIFYqi dong zi ou cun zai duo chong xie pai xiang ying yuan jian yu ji su xin hao diao kong yuan jian ,jing ding liang shi yan zheng shi PviTIFY10cheng yuan zai JAchu li ji yan yu gan han xie pai xia fan ying jiang lie ;JAZya jia zu bu fen cheng yuan you ji shi PviTIFY10b,zai hua xu yu gen de fa yo guo cheng zhong you jiao gao biao da 。gen ju PviTIFYde biao da te xing tui ce ji ke neng zai liu zhi ji ji su xin hao diao kong 、xie pai fang yu fan ying ji sheng chang fa yo guo cheng deng fang mian fa hui gong neng 。3.cheng gong ke long OsJAZ1yu OsTIFY10bde tong yuan ji yin PviTIFY3ayu PviTIFY10b;liang ge ji yin suo bian ma dan bai de bao shou jie gou yu zu cheng 、jin hua te dian 、li hua xing zhi ji er ji jie gou zu cheng deng fen zi te zheng xiang shi ;PviTIFY3a-GFProng ge dan bai shun shi biao da jie guo xian shi PviTIFY3ading wei yu xi bao he yu xi bao mo ,ruan jian yu ce PviTIFY10bhen ke neng ding wei yu xi bao zhi ;liang ge ji yin de guo biao da zai ti jun yi cheng gong gou jian 。
论文参考文献
论文详细介绍
论文作者分别是来自西北农林科技大学的赵晓晓，发表于刊物西北农林科技大学2019-07-11论文，是一篇关于柳枝稷论文,幼穗分化论文,基因家族论文,花序发育论文,基因克隆论文,西北农林科技大学2019-07-11论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自西北农林科技大学2019-07-11论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：柳枝稷论文; 幼穗分化论文; 基因家族论文; 花序发育论文; 基因克隆论文; 西北农林科技大学2019-07-11论文;