本文主要研究内容
作者薛珍珍（2019）在《基于次生代谢产物分析的厚朴道地性研究》一文中研究指出：道地药材是指在具有特定自然条件、生态环境的地域内所产的药材,且生产较为集中,栽培技术、采收加工也有一定的讲究,通常较同种药材在其他地区所产者品质佳、疗效好、为世所公认而久负盛名。几千年来,道地药材在人类防病治病过程中一直发挥着重要作用,是历代中医评价与控制中药材的一个独特的综合性标准。开展中药材的道地性研究是阐明道地药材与非道地药材在基因、性状、次生代谢产物等方面的品质差异、明确道地药材道地性成因的基础,并可为道地药材的引种栽培、可持续开发利用与保护提供参考。厚朴(Magnoliae Officinalis Cortex)是一味常见的理气化湿药,具有燥湿消痰、下气除满的功效。中药厚朴的来源较为复杂,2015版《中国药典》规定,木兰科植物厚朴Magnoliaofficinalis Rehd.et Wils.或凹叶厚朴M officinalis Rehd.et Wils.var.biloba Rehd.et Wils.为中药厚朴的基原植物,其干皮、根皮及枝皮均可入药。传统观点及历代本草文献认为,四川东部和湖北西部产的厚朴药材紫色油润,统称紫油厚朴或川朴,是著名的道地药材,质量最好。除此之外,浙江、福建、安徽、湖南等地也广布厚朴,且产量较大。目前对厚朴道地性的研究多是以挥发油和/或木脂素类成分厚朴酚、和厚朴酚为指标考察产地及种源对厚朴质量的影响,结论不一,但多数研究将产地及种源共同归结为引起挥发油及木脂素类成分差异的因素。而厚朴中除含有木脂素类成分和挥发油之外,还含有大量的苯乙醇苷、酚苷、生物碱等化学成分,仅以厚朴酚、和厚朴酚或者挥发油为指标难以体现中药的质量,进行道地性研究得出的结论也较片面。因此本论文采用2LC-ECD、UPLC-QTOF/MS以及GC-MS技术,分别对厚朴中水溶性成分-阿洛糖苯乙醇苷类成分、非挥发性次生代谢产物以及挥发油成分进行分析,比较厚朴道地产区与非道地产区厚朴样本中次生代谢产物的差异;结合产区土壤和气候因子的影响,探讨厚朴道地性形成的模式。1)厚朴水溶性化学成分及生物活性研究厚朴药材传统上多水煎入药,现代药理研究表明厚朴水煎剂能缓解实验性动物胃肠动力障碍。本论文采用D101大孔树脂、MCI、ODS、Sephadex LH-20以及高效液相制备色谱等技术对厚朴水溶性化学成分进行分离纯化,通过化学方法和波谱技术(UV,IR,MS,1HNMR,13CNMR,HSQC,HMBC,NOESY等)对化合物进行结构鉴定,从厚朴中分离并鉴定出阿洛糖苯乙醇苷类化合物10个,木脂素苷、酚苷类化合物7个。其中11个为新化合物,1个首次从该植物中分离得到。药理活性结果表明部分阿洛糖苯乙醇苷类化合物对α-葡萄糖苷酶有较好的抑制活性以及细胞毒性。2)基于2LC-ECD技术的厚朴阿洛糖苯乙醇苷成分定量分析首次建立了以高灵敏度的2LC-ECD色谱技术系统,测定厚朴中7个阿洛糖苯乙醇苷含量的方法,并以阿洛糖苯乙醇苷含量为指标,采用化学计量学方法分析了道地与非道地产区厚朴样本阿洛糖苯乙醇苷的异同。研究结果表明,道地产区的样本与非道地产区的样本(包括厚朴和凹叶厚朴)各自聚类,说明产地的影响要大于种源;道地产区厚朴样本中苯乙醇二糖苷的含量显著高于非道地产区者,苯乙醇三糖苷的含量显著低于非道地产区者;其中,木兰苷D和木兰苷M是区别道地与非道地产区厚朴样本的重要指标。3)基于UPLC-QTOF/MS技术的厚朴次生代谢产物分析采用UPLC-QTOF/MS技术,首次建立了诊断离子/中性丢失扫描结合化合物全碎片离子指纹图谱分析的方法,初步判定了厚朴苯乙醇苷类化合物101个,木脂素(苷)、酚苷类化合物69个,生物碱类化合物30个;建立了苯乙醇苷类化合物空间异构体及位置异构体的判别模型,快速有效地判定了厚朴中14个苯乙醇苷异构体。同时,建立了厚朴次生代谢产物数据库,对厚朴道地性进行了研究,发现道地产区与非道地产区厚朴样本中苯乙醇苷类成分、木脂素类成分以及生物碱类成分存在明显的差异,其中阿洛糖苯乙醇四糖苷(木兰苷N异构体)、木脂素化合物(magnolignan E)、生物碱化合物(1,2-hydrazinedicarboxamide)在厚朴道地与非道地产区样本中相对含量差异显著,道地产区的样本与非道地产区者(包括厚朴和凹叶厚朴)各自聚类。同时,此类差异也存在于厚朴花及厚朴叶样本中,进一步佐证了道地产区与非道地产区样本在化学成分上的差异。4)基于GC-MS技术的厚朴挥发油成分分析对课题组前期采集的厚朴挥发油GC-MS数据进行多变量统计分析,开展基于挥发油成分分析的厚朴道地性研究。研究结果表明,道地与非道地产区厚朴样本中挥发油成分存在显著差异,其中α-沉香呋喃、Y-桉叶醇及β-桉叶醇在非道地产区厚朴样本中的相对含量显著高于道地产区者。5)产区环境因子对化学成分的影响采用双变量相关分析的方法,对产区土壤、气候因子与厚朴样本各类成分的相关性进行分析,研究结果表明,不同产区厚朴样本中各次生代谢产物的差异与各产区的气温和降水量因子显著相关。同时,结合前期的研究结果,提出厚朴道地性形成的模式为多因子关联决定型,即受种源及产地二者的共同影响。综上,针对厚朴中化学成分的特点,建立了 2LC-ECD、UPLC-QTOF/MS、GC-MS方法,对厚朴中水溶性成分-阿洛糖苯乙醇苷类化合物、各非挥发性以及挥发性次生代谢产物进行了系统的研究,建立了厚朴次生代谢产物库;发现道地产区与非道地产区厚朴样本中次生代谢成分存在显著差异,道地产区与非道地产区样本各自聚类,阿洛糖苯乙醇二糖苷、三糖苷,阿洛糖苯乙醇四糖苷(木兰苷N异构体)、木脂素化合物(magnolignan E)、生物碱化合物(1,2-hydrazinedicarboxamide),以及挥发油(α-沉香呋喃、γ-桉叶醇、β-桉叶醇)在厚朴的道地性判别中贡献较大;且不同产区厚朴样本中各次生代谢产物的差异与各产区的气温和降水量因子显著相关。同时,结合前期的研究结果,提出厚朴道地性形成的模式为多因子关联决定型,即受种源及产地二者的共同影响。
Abstract
dao de yao cai shi zhi zai ju you te ding zi ran tiao jian 、sheng tai huan jing de de yu nei suo chan de yao cai ,ju sheng chan jiao wei ji zhong ,zai pei ji shu 、cai shou jia gong ye you yi ding de jiang jiu ,tong chang jiao tong chong yao cai zai ji ta de ou suo chan zhe pin zhi jia 、liao xiao hao 、wei shi suo gong ren er jiu fu cheng ming 。ji qian nian lai ,dao de yao cai zai ren lei fang bing zhi bing guo cheng zhong yi zhi fa hui zhao chong yao zuo yong ,shi li dai zhong yi ping jia yu kong zhi zhong yao cai de yi ge du te de zeng ge xing biao zhun 。kai zhan zhong yao cai de dao de xing yan jiu shi chan ming dao de yao cai yu fei dao de yao cai zai ji yin 、xing zhuang 、ci sheng dai xie chan wu deng fang mian de pin zhi cha yi 、ming que dao de yao cai dao de xing cheng yin de ji chu ,bing ke wei dao de yao cai de yin chong zai pei 、ke chi xu kai fa li yong yu bao hu di gong can kao 。hou piao (Magnoliae Officinalis Cortex)shi yi wei chang jian de li qi hua shi yao ,ju you zao shi xiao tan 、xia qi chu man de gong xiao 。zhong yao hou piao de lai yuan jiao wei fu za ,2015ban 《zhong guo yao dian 》gui ding ,mu lan ke zhi wu hou piao Magnoliaofficinalis Rehd.et Wils.huo ao xie hou piao M officinalis Rehd.et Wils.var.biloba Rehd.et Wils.wei zhong yao hou piao de ji yuan zhi wu ,ji gan pi 、gen pi ji zhi pi jun ke ru yao 。chuan tong guan dian ji li dai ben cao wen suo ren wei ,si chuan dong bu he hu bei xi bu chan de hou piao yao cai zi se you run ,tong chen zi you hou piao huo chuan piao ,shi zhe ming de dao de yao cai ,zhi liang zui hao 。chu ci zhi wai ,zhe jiang 、fu jian 、an hui 、hu na deng de ye an bu hou piao ,ju chan liang jiao da 。mu qian dui hou piao dao de xing de yan jiu duo shi yi hui fa you he /huo mu zhi su lei cheng fen hou piao fen 、he hou piao fen wei zhi biao kao cha chan de ji chong yuan dui hou piao zhi liang de ying xiang ,jie lun bu yi ,dan duo shu yan jiu jiang chan de ji chong yuan gong tong gui jie wei yin qi hui fa you ji mu zhi su lei cheng fen cha yi de yin su 。er hou piao zhong chu han you mu zhi su lei cheng fen he hui fa you zhi wai ,hai han you da liang de ben yi chun gan 、fen gan 、sheng wu jian deng hua xue cheng fen ,jin yi hou piao fen 、he hou piao fen huo zhe hui fa you wei zhi biao nan yi ti xian zhong yao de zhi liang ,jin hang dao de xing yan jiu de chu de jie lun ye jiao pian mian 。yin ci ben lun wen cai yong 2LC-ECD、UPLC-QTOF/MSyi ji GC-MSji shu ,fen bie dui hou piao zhong shui rong xing cheng fen -a luo tang ben yi chun gan lei cheng fen 、fei hui fa xing ci sheng dai xie chan wu yi ji hui fa you cheng fen jin hang fen xi ,bi jiao hou piao dao de chan ou yu fei dao de chan ou hou piao yang ben zhong ci sheng dai xie chan wu de cha yi ;jie ge chan ou tu rang he qi hou yin zi de ying xiang ,tan tao hou piao dao de xing xing cheng de mo shi 。1)hou piao shui rong xing hua xue cheng fen ji sheng wu huo xing yan jiu hou piao yao cai chuan tong shang duo shui jian ru yao ,xian dai yao li yan jiu biao ming hou piao shui jian ji neng huan jie shi yan xing dong wu wei chang dong li zhang ai 。ben lun wen cai yong D101da kong shu zhi 、MCI、ODS、Sephadex LH-20yi ji gao xiao ye xiang zhi bei se pu deng ji shu dui hou piao shui rong xing hua xue cheng fen jin hang fen li chun hua ,tong guo hua xue fang fa he bo pu ji shu (UV,IR,MS,1HNMR,13CNMR,HSQC,HMBC,NOESYdeng )dui hua ge wu jin hang jie gou jian ding ,cong hou piao zhong fen li bing jian ding chu a luo tang ben yi chun gan lei hua ge wu 10ge ,mu zhi su gan 、fen gan lei hua ge wu 7ge 。ji zhong 11ge wei xin hua ge wu ,1ge shou ci cong gai zhi wu zhong fen li de dao 。yao li huo xing jie guo biao ming bu fen a luo tang ben yi chun gan lei hua ge wu dui α-pu tao tang gan mei you jiao hao de yi zhi huo xing yi ji xi bao du xing 。2)ji yu 2LC-ECDji shu de hou piao a luo tang ben yi chun gan cheng fen ding liang fen xi shou ci jian li le yi gao ling min du de 2LC-ECDse pu ji shu ji tong ,ce ding hou piao zhong 7ge a luo tang ben yi chun gan han liang de fang fa ,bing yi a luo tang ben yi chun gan han liang wei zhi biao ,cai yong hua xue ji liang xue fang fa fen xi le dao de yu fei dao de chan ou hou piao yang ben a luo tang ben yi chun gan de yi tong 。yan jiu jie guo biao ming ,dao de chan ou de yang ben yu fei dao de chan ou de yang ben (bao gua hou piao he ao xie hou piao )ge zi ju lei ,shui ming chan de de ying xiang yao da yu chong yuan ;dao de chan ou hou piao yang ben zhong ben yi chun er tang gan de han liang xian zhe gao yu fei dao de chan ou zhe ,ben yi chun san tang gan de han liang xian zhe di yu fei dao de chan ou zhe ;ji zhong ,mu lan gan Dhe mu lan gan Mshi ou bie dao de yu fei dao de chan ou hou piao yang ben de chong yao zhi biao 。3)ji yu UPLC-QTOF/MSji shu de hou piao ci sheng dai xie chan wu fen xi cai yong UPLC-QTOF/MSji shu ,shou ci jian li le zhen duan li zi /zhong xing diu shi sao miao jie ge hua ge wu quan sui pian li zi zhi wen tu pu fen xi de fang fa ,chu bu pan ding le hou piao ben yi chun gan lei hua ge wu 101ge ,mu zhi su (gan )、fen gan lei hua ge wu 69ge ,sheng wu jian lei hua ge wu 30ge ;jian li le ben yi chun gan lei hua ge wu kong jian yi gou ti ji wei zhi yi gou ti de pan bie mo xing ,kuai su you xiao de pan ding le hou piao zhong 14ge ben yi chun gan yi gou ti 。tong shi ,jian li le hou piao ci sheng dai xie chan wu shu ju ku ,dui hou piao dao de xing jin hang le yan jiu ,fa xian dao de chan ou yu fei dao de chan ou hou piao yang ben zhong ben yi chun gan lei cheng fen 、mu zhi su lei cheng fen yi ji sheng wu jian lei cheng fen cun zai ming xian de cha yi ,ji zhong a luo tang ben yi chun si tang gan (mu lan gan Nyi gou ti )、mu zhi su hua ge wu (magnolignan E)、sheng wu jian hua ge wu (1,2-hydrazinedicarboxamide)zai hou piao dao de yu fei dao de chan ou yang ben zhong xiang dui han liang cha yi xian zhe ,dao de chan ou de yang ben yu fei dao de chan ou zhe (bao gua hou piao he ao xie hou piao )ge zi ju lei 。tong shi ,ci lei cha yi ye cun zai yu hou piao hua ji hou piao xie yang ben zhong ,jin yi bu zuo zheng le dao de chan ou yu fei dao de chan ou yang ben zai hua xue cheng fen shang de cha yi 。4)ji yu GC-MSji shu de hou piao hui fa you cheng fen fen xi dui ke ti zu qian ji cai ji de hou piao hui fa you GC-MSshu ju jin hang duo bian liang tong ji fen xi ,kai zhan ji yu hui fa you cheng fen fen xi de hou piao dao de xing yan jiu 。yan jiu jie guo biao ming ,dao de yu fei dao de chan ou hou piao yang ben zhong hui fa you cheng fen cun zai xian zhe cha yi ,ji zhong α-chen xiang fu nan 、Y-an xie chun ji β-an xie chun zai fei dao de chan ou hou piao yang ben zhong de xiang dui han liang xian zhe gao yu dao de chan ou zhe 。5)chan ou huan jing yin zi dui hua xue cheng fen de ying xiang cai yong shuang bian liang xiang guan fen xi de fang fa ,dui chan ou tu rang 、qi hou yin zi yu hou piao yang ben ge lei cheng fen de xiang guan xing jin hang fen xi ,yan jiu jie guo biao ming ,bu tong chan ou hou piao yang ben zhong ge ci sheng dai xie chan wu de cha yi yu ge chan ou de qi wen he jiang shui liang yin zi xian zhe xiang guan 。tong shi ,jie ge qian ji de yan jiu jie guo ,di chu hou piao dao de xing xing cheng de mo shi wei duo yin zi guan lian jue ding xing ,ji shou chong yuan ji chan de er zhe de gong tong ying xiang 。zeng shang ,zhen dui hou piao zhong hua xue cheng fen de te dian ,jian li le 2LC-ECD、UPLC-QTOF/MS、GC-MSfang fa ,dui hou piao zhong shui rong xing cheng fen -a luo tang ben yi chun gan lei hua ge wu 、ge fei hui fa xing yi ji hui fa xing ci sheng dai xie chan wu jin hang le ji tong de yan jiu ,jian li le hou piao ci sheng dai xie chan wu ku ;fa xian dao de chan ou yu fei dao de chan ou hou piao yang ben zhong ci sheng dai xie cheng fen cun zai xian zhe cha yi ,dao de chan ou yu fei dao de chan ou yang ben ge zi ju lei ,a luo tang ben yi chun er tang gan 、san tang gan ,a luo tang ben yi chun si tang gan (mu lan gan Nyi gou ti )、mu zhi su hua ge wu (magnolignan E)、sheng wu jian hua ge wu (1,2-hydrazinedicarboxamide),yi ji hui fa you (α-chen xiang fu nan 、γ-an xie chun 、β-an xie chun )zai hou piao de dao de xing pan bie zhong gong suo jiao da ;ju bu tong chan ou hou piao yang ben zhong ge ci sheng dai xie chan wu de cha yi yu ge chan ou de qi wen he jiang shui liang yin zi xian zhe xiang guan 。tong shi ,jie ge qian ji de yan jiu jie guo ,di chu hou piao dao de xing xing cheng de mo shi wei duo yin zi guan lian jue ding xing ,ji shou chong yuan ji chan de er zhe de gong tong ying xiang 。
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论文详细介绍
论文作者分别是来自中国中医科学院的薛珍珍，发表于刊物中国中医科学院2019-10-28论文，是一篇关于厚朴论文,道地性论文,阿洛糖苯乙醇苷论文,次生代谢产物论文,挥发油论文,电化学检测论文,中国中医科学院2019-10-28论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自中国中医科学院2019-10-28论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：厚朴论文; 道地性论文; 阿洛糖苯乙醇苷论文; 次生代谢产物论文; 挥发油论文; 电化学检测论文; 中国中医科学院2019-10-28论文;