本文主要研究内容
作者高晓燕（2019）在《几种天然产物的双水相体系萃取研究》一文中研究指出：天然产物无处不在,种类繁多,广泛应用于药物、食品、化妆品、保健品以及颜料等行业。经过多年研究,有机溶剂-盐双水相体系萃取天然产物已逐渐成熟。前人在寻找萃取某种天然产物最优实验条件需要大量的工作,且不同的研究者萃取同一物质所选的体系各不相同,故每次在萃取新植物中的某类天然产物时都需要诸多尝试,实验过程繁复,不利于大规模生产。因此,探求双水相萃取各类天然产物最优体系之间的规律性格外重要。本文在大量查阅现有文献的基础上,对多种天然产物及其双水相萃取体系进行了分析,以此为基础,以天然产物中最为广泛的三类物质皂苷类、黄酮类、生物碱类作为研究对象,选取桔梗皂苷、金银花黄酮和辣椒碱作为研究的具体物质。通过比较四种有机溶剂与多种无机盐构成的双水相体系成相能力,初选出乙醇、异丙醇、丙酮和无机盐（（NH4）2SO4、NaH2PO4、K2HPO4）作为双水相萃取此三种天然产物的待考察体系组成。通过双水相体系萃取三种天然产物的实验,得出最优萃取实验条件,实验结果表明:双水相萃取桔梗总皂苷的最优条件为:25%乙醇-24%NaH2PO4（质量分数）,pH3.92,料液比1:200,分配系数和回收率分别为13.25和94.85%,得率为43.75 mg/g;双水相萃取金银花中总黄酮的最优条件为:25%乙醇-28%NaH2PO4（质量分数）,pH=3.96,料液比1:100,分配系数和回收率分别为206.48和99.57%,得率为109.75 mg/g;双水相萃取辣椒中辣椒碱的最优条件为:25%丙酮-24%K2HPO4（质量分数）,pH=10.03,料液比1:100,分配系数和回收率分别为184.80和99.47%,得率为8.04 mg/g。通过分析天然产物结构与所选最优萃取体系的关系,发现:双水相体系组成中有机溶剂的选择与产物的极性有关,盐的选择多与本身的成相能力和待萃物酸碱性有关。通过液相色谱,分别对三种双水相萃取所得产品初步纯化分析。液相色谱分离纯化桔梗皂苷产品和金银花黄酮类物质均采用反相液相色谱乙腈-0.2%磷酸水溶液梯度洗脱方式。仅通过双水相萃取和制备色谱纯化,无法得到桔梗皂苷D纯品;由于有绿原酸类物质影响,无法得到芦丁和木犀草苷单品。液相色谱分离纯化辣椒碱类物质采用甲醇-水洗脱方式,通过双水相萃取和制备色谱纯化两步,辣椒碱在纯化后产品中纯度较高。
Abstract
tian ran chan wu mo chu bu zai ,chong lei fan duo ,an fan ying yong yu yao wu 、shi pin 、hua zhuang pin 、bao jian pin yi ji yan liao deng hang ye 。jing guo duo nian yan jiu ,you ji rong ji -yan shuang shui xiang ti ji cui qu tian ran chan wu yi zhu jian cheng shou 。qian ren zai xun zhao cui qu mou chong tian ran chan wu zui you shi yan tiao jian xu yao da liang de gong zuo ,ju bu tong de yan jiu zhe cui qu tong yi wu zhi suo shua de ti ji ge bu xiang tong ,gu mei ci zai cui qu xin zhi wu zhong de mou lei tian ran chan wu shi dou xu yao zhu duo chang shi ,shi yan guo cheng fan fu ,bu li yu da gui mo sheng chan 。yin ci ,tan qiu shuang shui xiang cui qu ge lei tian ran chan wu zui you ti ji zhi jian de gui lv xing ge wai chong yao 。ben wen zai da liang cha yue xian you wen suo de ji chu shang ,dui duo chong tian ran chan wu ji ji shuang shui xiang cui qu ti ji jin hang le fen xi ,yi ci wei ji chu ,yi tian ran chan wu zhong zui wei an fan de san lei wu zhi zao gan lei 、huang tong lei 、sheng wu jian lei zuo wei yan jiu dui xiang ,shua qu jie geng zao gan 、jin yin hua huang tong he la jiao jian zuo wei yan jiu de ju ti wu zhi 。tong guo bi jiao si chong you ji rong ji yu duo chong mo ji yan gou cheng de shuang shui xiang ti ji cheng xiang neng li ,chu shua chu yi chun 、yi bing chun 、bing tong he mo ji yan （（NH4）2SO4、NaH2PO4、K2HPO4）zuo wei shuang shui xiang cui qu ci san chong tian ran chan wu de dai kao cha ti ji zu cheng 。tong guo shuang shui xiang ti ji cui qu san chong tian ran chan wu de shi yan ,de chu zui you cui qu shi yan tiao jian ,shi yan jie guo biao ming :shuang shui xiang cui qu jie geng zong zao gan de zui you tiao jian wei :25%yi chun -24%NaH2PO4（zhi liang fen shu ）,pH3.92,liao ye bi 1:200,fen pei ji shu he hui shou lv fen bie wei 13.25he 94.85%,de lv wei 43.75 mg/g;shuang shui xiang cui qu jin yin hua zhong zong huang tong de zui you tiao jian wei :25%yi chun -28%NaH2PO4（zhi liang fen shu ）,pH=3.96,liao ye bi 1:100,fen pei ji shu he hui shou lv fen bie wei 206.48he 99.57%,de lv wei 109.75 mg/g;shuang shui xiang cui qu la jiao zhong la jiao jian de zui you tiao jian wei :25%bing tong -24%K2HPO4（zhi liang fen shu ）,pH=10.03,liao ye bi 1:100,fen pei ji shu he hui shou lv fen bie wei 184.80he 99.47%,de lv wei 8.04 mg/g。tong guo fen xi tian ran chan wu jie gou yu suo shua zui you cui qu ti ji de guan ji ,fa xian :shuang shui xiang ti ji zu cheng zhong you ji rong ji de shua ze yu chan wu de ji xing you guan ,yan de shua ze duo yu ben shen de cheng xiang neng li he dai cui wu suan jian xing you guan 。tong guo ye xiang se pu ,fen bie dui san chong shuang shui xiang cui qu suo de chan pin chu bu chun hua fen xi 。ye xiang se pu fen li chun hua jie geng zao gan chan pin he jin yin hua huang tong lei wu zhi jun cai yong fan xiang ye xiang se pu yi jing -0.2%lin suan shui rong ye ti du xi tuo fang shi 。jin tong guo shuang shui xiang cui qu he zhi bei se pu chun hua ,mo fa de dao jie geng zao gan Dchun pin ;you yu you lu yuan suan lei wu zhi ying xiang ,mo fa de dao lu ding he mu xi cao gan chan pin 。ye xiang se pu fen li chun hua la jiao jian lei wu zhi cai yong jia chun -shui xi tuo fang shi ,tong guo shuang shui xiang cui qu he zhi bei se pu chun hua liang bu ,la jiao jian zai chun hua hou chan pin zhong chun du jiao gao 。
论文参考文献
论文详细介绍
论文作者分别是来自中国矿业大学的高晓燕，发表于刊物中国矿业大学2019-07-18论文，是一篇关于桔梗皂苷论文,金银花总黄酮论文,辣椒碱类物质论文,双水相论文,萃取论文,中国矿业大学2019-07-18论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自中国矿业大学2019-07-18论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：桔梗皂苷论文; 金银花总黄酮论文; 辣椒碱类物质论文; 双水相论文; 萃取论文; 中国矿业大学2019-07-18论文;