本文主要研究内容
作者马艳（2019）在《山核桃天牛植物源引诱剂的初步研究》一文中研究指出：桑天牛Apriona germari、星天牛Anoplophora chinensis和云斑天牛Batocera horsfieldi是3种为害山核桃Carya cathayensis最严重的天牛,其成虫啃食新枝嫩梢,幼虫蛀食山核桃树枝和枝干,为害时间长且隐蔽,难以防治,且目前对这3种天牛植物源引诱剂研究较少。故本文通过活体植物动态顶空套袋吸附法收集不同危害状态[健康(CK)以及天牛取食、产卵和钻蛀危害]下山核桃释放的挥发物,并利用气相色谱-质谱联用仪(GC-MS)、气相色谱-触角电位测量系统(GC-EAD)及Y型嗅觉仪分析鉴定出对桑天牛、星天牛和云斑天牛成虫产生电生理及行为反应的挥发物,最后通过林间诱捕试验来验证对这3种天牛具有行为反应的挥发物,为筛选桑天牛、星天牛和云斑天牛的植物源引诱剂的成分和比例提供理论支撑。研究发现:1、天牛胁迫对山核桃挥发物有一定的影响。不同危害状态下山核桃释放的挥发物分别鉴定出45,37,35和46种化合物,共7类51种,皆以萜烯类和芳香族化合物居多,其中7类33种是共有挥发物。但每种危害状态下山核桃释放的挥发物都有一种或几种特有的成分,且不同危害状态下山核桃释放的挥发物多种成分存在显著性差异,其中受到天牛幼虫钻蛀危害的山核桃释放的挥发物与健康山核桃的相似,而受到天牛成虫取食和产卵危害与健康山核桃存在较大差异。2、桑天牛、星天牛和云斑天牛对不同危害状态下山核桃挥发物的GC-EAD反应存在差异,其中桑天牛成虫仅对不同危害状态下山核桃释放的蒎烯、乙酸叶醇酯、壬醛、α-萜品醇、丙烯酸-2-乙基己酯及正十六烷6种挥发物产生明显的电生理反应;星天牛成虫仅对蒎烯、罗勒烯、萜品烯、对二乙苯、壬醛、α-萜品醇、五甲基苯、丙烯酸-2-乙基己酯、2,6-二甲基萘及正十六烷这10种挥发物有明显的电生理反应;而云斑天牛成虫仅对蒎烯、萜品烯、壬醛、α-萜品醇、五甲基苯、丙烯酸-2-乙基己酯、2,6-二甲基萘及正十六烷这8种挥发物有明显的电生理反应。3、嗅觉反应结果表明,乙酸叶醇酯、壬醛和丙烯酸-2-乙基己酯对桑天牛成虫有较强的引诱作用,而α-萜品醇产生较好的驱避作用;罗勒烯对星天牛雌成虫产生较强的引诱作用,而丙烯酸-2-乙基己酯产生较好的驱避作用,蒎烯和罗勒烯对星天牛雄成虫产生较强的引诱作用,而对二乙苯和壬醛产生较好的驱避作用;壬醛和α-萜品醇对云斑天牛雌成虫产生较强的引诱作用,而蒎烯产生较好的驱避作用,萜品烯和壬醛对云斑天牛雄成虫产生较强的引诱作用,而α-萜品醇产生较好的驱避作用。4、林间诱捕试验结果表明,乙酸叶醇酯对桑天牛的引诱效果最好,其次是壬醛和丙烯酸-2-乙基己酯,且诱捕效果没有显著差异(P<0.05),其中乙酸叶醇酯和壬醛诱捕到的雄虫比例更高,而丙烯酸-2-乙基己酯诱捕到的雌虫比例更高;罗勒烯对星天牛的诱捕效果显著高于其他诱芯(P<0.05),其次是β-蒎烯、壬醛、月桂烯和蒎烯,其中蒎烯、壬醛、β-蒎烯和月桂烯诱捕到的雄虫比例更高,而罗勒烯诱捕到的雌虫比例更高;壬醛和顺-3-己烯-1-醇对云斑天牛的诱捕效果显著高于其他诱芯(P<0.05),其次是α-萜品醇、萜品烯和E-2-辛烯醛,其中萜品烯诱捕到都是雄虫,E-2-辛烯醛诱捕到的雄虫比例更高,而壬醛、α-萜品醇和顺-3-己烯-1-醇诱捕到的雌虫比例更高。综上,仅有壬醛同时诱捕到桑天牛、星天牛和云斑天牛,说明壬醛是这3种天牛的共享植物源引诱挥发物。
Abstract
sang tian niu Apriona germari、xing tian niu Anoplophora chinensishe yun ban tian niu Batocera horsfieldishi 3chong wei hai shan he tao Carya cathayensiszui yan chong de tian niu ,ji cheng chong ken shi xin zhi nen sao ,you chong zhu shi shan he tao shu zhi he zhi gan ,wei hai shi jian chang ju yin bi ,nan yi fang zhi ,ju mu qian dui zhe 3chong tian niu zhi wu yuan yin you ji yan jiu jiao shao 。gu ben wen tong guo huo ti zhi wu dong tai ding kong tao dai xi fu fa shou ji bu tong wei hai zhuang tai [jian kang (CK)yi ji tian niu qu shi 、chan luan he zuan zhu wei hai ]xia shan he tao shi fang de hui fa wu ,bing li yong qi xiang se pu -zhi pu lian yong yi (GC-MS)、qi xiang se pu -chu jiao dian wei ce liang ji tong (GC-EAD)ji Yxing xiu jiao yi fen xi jian ding chu dui sang tian niu 、xing tian niu he yun ban tian niu cheng chong chan sheng dian sheng li ji hang wei fan ying de hui fa wu ,zui hou tong guo lin jian you bu shi yan lai yan zheng dui zhe 3chong tian niu ju you hang wei fan ying de hui fa wu ,wei shai shua sang tian niu 、xing tian niu he yun ban tian niu de zhi wu yuan yin you ji de cheng fen he bi li di gong li lun zhi cheng 。yan jiu fa xian :1、tian niu xie pai dui shan he tao hui fa wu you yi ding de ying xiang 。bu tong wei hai zhuang tai xia shan he tao shi fang de hui fa wu fen bie jian ding chu 45,37,35he 46chong hua ge wu ,gong 7lei 51chong ,jie yi tie xi lei he fang xiang zu hua ge wu ju duo ,ji zhong 7lei 33chong shi gong you hui fa wu 。dan mei chong wei hai zhuang tai xia shan he tao shi fang de hui fa wu dou you yi chong huo ji chong te you de cheng fen ,ju bu tong wei hai zhuang tai xia shan he tao shi fang de hui fa wu duo chong cheng fen cun zai xian zhe xing cha yi ,ji zhong shou dao tian niu you chong zuan zhu wei hai de shan he tao shi fang de hui fa wu yu jian kang shan he tao de xiang shi ,er shou dao tian niu cheng chong qu shi he chan luan wei hai yu jian kang shan he tao cun zai jiao da cha yi 。2、sang tian niu 、xing tian niu he yun ban tian niu dui bu tong wei hai zhuang tai xia shan he tao hui fa wu de GC-EADfan ying cun zai cha yi ,ji zhong sang tian niu cheng chong jin dui bu tong wei hai zhuang tai xia shan he tao shi fang de pai xi 、yi suan xie chun zhi 、ren quan 、α-tie pin chun 、bing xi suan -2-yi ji ji zhi ji zheng shi liu wan 6chong hui fa wu chan sheng ming xian de dian sheng li fan ying ;xing tian niu cheng chong jin dui pai xi 、luo le xi 、tie pin xi 、dui er yi ben 、ren quan 、α-tie pin chun 、wu jia ji ben 、bing xi suan -2-yi ji ji zhi 、2,6-er jia ji nai ji zheng shi liu wan zhe 10chong hui fa wu you ming xian de dian sheng li fan ying ;er yun ban tian niu cheng chong jin dui pai xi 、tie pin xi 、ren quan 、α-tie pin chun 、wu jia ji ben 、bing xi suan -2-yi ji ji zhi 、2,6-er jia ji nai ji zheng shi liu wan zhe 8chong hui fa wu you ming xian de dian sheng li fan ying 。3、xiu jiao fan ying jie guo biao ming ,yi suan xie chun zhi 、ren quan he bing xi suan -2-yi ji ji zhi dui sang tian niu cheng chong you jiao jiang de yin you zuo yong ,er α-tie pin chun chan sheng jiao hao de qu bi zuo yong ;luo le xi dui xing tian niu ci cheng chong chan sheng jiao jiang de yin you zuo yong ,er bing xi suan -2-yi ji ji zhi chan sheng jiao hao de qu bi zuo yong ,pai xi he luo le xi dui xing tian niu xiong cheng chong chan sheng jiao jiang de yin you zuo yong ,er dui er yi ben he ren quan chan sheng jiao hao de qu bi zuo yong ;ren quan he α-tie pin chun dui yun ban tian niu ci cheng chong chan sheng jiao jiang de yin you zuo yong ,er pai xi chan sheng jiao hao de qu bi zuo yong ,tie pin xi he ren quan dui yun ban tian niu xiong cheng chong chan sheng jiao jiang de yin you zuo yong ,er α-tie pin chun chan sheng jiao hao de qu bi zuo yong 。4、lin jian you bu shi yan jie guo biao ming ,yi suan xie chun zhi dui sang tian niu de yin you xiao guo zui hao ,ji ci shi ren quan he bing xi suan -2-yi ji ji zhi ,ju you bu xiao guo mei you xian zhe cha yi (P<0.05),ji zhong yi suan xie chun zhi he ren quan you bu dao de xiong chong bi li geng gao ,er bing xi suan -2-yi ji ji zhi you bu dao de ci chong bi li geng gao ;luo le xi dui xing tian niu de you bu xiao guo xian zhe gao yu ji ta you xin (P<0.05),ji ci shi β-pai xi 、ren quan 、yue gui xi he pai xi ,ji zhong pai xi 、ren quan 、β-pai xi he yue gui xi you bu dao de xiong chong bi li geng gao ,er luo le xi you bu dao de ci chong bi li geng gao ;ren quan he shun -3-ji xi -1-chun dui yun ban tian niu de you bu xiao guo xian zhe gao yu ji ta you xin (P<0.05),ji ci shi α-tie pin chun 、tie pin xi he E-2-xin xi quan ,ji zhong tie pin xi you bu dao dou shi xiong chong ,E-2-xin xi quan you bu dao de xiong chong bi li geng gao ,er ren quan 、α-tie pin chun he shun -3-ji xi -1-chun you bu dao de ci chong bi li geng gao 。zeng shang ,jin you ren quan tong shi you bu dao sang tian niu 、xing tian niu he yun ban tian niu ,shui ming ren quan shi zhe 3chong tian niu de gong xiang zhi wu yuan yin you hui fa wu 。
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论文作者分别是来自浙江农林大学的马艳，发表于刊物浙江农林大学2019-10-24论文，是一篇关于山核桃挥发物论文,桑天牛论文,星天牛论文,云斑天牛论文,型嗅觉仪论文,诱捕论文,浙江农林大学2019-10-24论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自浙江农林大学2019-10-24论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：山核桃挥发物论文; 桑天牛论文; 星天牛论文; 云斑天牛论文; 型嗅觉仪论文; 诱捕论文; 浙江农林大学2019-10-24论文;