论文摘要
研究目的本研究通过阻塞大鼠大脑中动脉,建立局灶性脑缺血的动物模型,并观察脑缺血时大脑皮质、海马区脑源性神经营养因子（BDNF）含量的表达变化。研究方法雄性SD大鼠随机分为假手术组和缺&6h组、缺血12h组、缺血24h组、缺血48h组,共5组,每组6只。用大鼠大脑中动脉线栓法（MCAO）建立脑缺血模型,采用免疫组织化学、RT-PCR和Western-blot方法观察BDNF在不同时间节点的表达变化。研究结果采用单因素方差分析组间差异,假手术组与局灶性缺血6h、12h组比较BDNF的表达没有显著性差异,但与局灶性缺血24h及48h组比较脑中BDNF表达明显升高（P＜0.05,P＜0.01）;模型组间比较缺血24h、48h组与缺血6h、12h组BDNF表达也有显著性差异（P＜0.05）。研究结论脑缺血后神经元内BDNF表达量增多,推测可能有利于受损神经元的修复。
论文目录
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