本文主要研究内容
作者马聚泽（2019）在《山楂自交亲和性突变体‘紫珍珠’的遗传分析》一文中研究指出：山楂（Crataegus pinnatifida Bge.）是蔷薇科（Rosaceae）山楂属（Crataegus spp.）果树。作为我国重要的果树种质资源之一,其自交不亲和机制的研究尚未见报道,而‘紫珍珠’作为目前发现的唯一山楂自交亲和性的种质资源,为探究山楂自交亲不和性的分子机制及山楂属植物的自交不亲和机制提供了重要的研究材料。本研究一方面利用蔷薇科果树基因组数据库对核酸酶数据进行生物信息学分析,分别在5’UTR和3’UTR区域设计引物克隆‘紫珍珠’S-RNase基因,并通过NCBI数据库、Lasergene、MEGA7.0和SWISS MODLE软件进行同源比对、结构分析、系统进化树分析和蛋白三级结构分析;另一方面设计‘紫珍珠’的自交及杂交授粉试验,通过对自交和杂交后代群体的遗传分析,来讨论其自交亲和性突变的原因。结果如下:（1）从‘紫珍珠’中克隆出两条长度分别为960 bp和843 bp的全长S-RNase基因序列,分别命名为ZZZS1-RNase和ZZZS2-RNase。登录到GeneBank的ID分别为MG385836和MG385837。二者均属于RNase T2基因家族,推导的氨基酸序列具有与梨属、苹果属S-RNase相似的保守结构。系统进化树中,ZZZS1-RNase和ZZZS2-RNase与苹果属和梨属聚类在一起,提示山楂属自交不亲和类型可能与苹果属、梨属为同一类型。（2）‘紫珍珠’及其自交后代‘6号’均为S1S2基因型,‘紫珍珠’自花授粉亲和指数为1.27,表现自交亲和;自交后代‘6号’自花授粉亲和指数为0.57,表现自交不亲和,相互授粉发现‘紫珍珠’给‘6号’授粉时亲和指数为0,表现自交不亲和;而‘6号’做父本给‘紫珍珠’授粉时,亲和指数为0.965,表现自交亲和。初步判断‘紫珍珠’自交亲和性突变发生在花柱部分。（3）利用‘紫珍珠’两条等位基因的序列多态性,设计了2对特异性引物对自交及杂交后代群体S基因型进行鉴定,34个自交后代中出现了三种基因型S1S1、S1S2和S2S2（3:26:5）,出现了偏分离现象;‘紫珍珠×6号’34个杂交后代的三种基因型S1S1、S1S2和S2S2比例为7:16:11（≈1:2:1）,符合孟得尔遗传规律,综上所述,推断‘紫珍珠’自交亲和性是由花柱部分非S因子突变导致。（4）通过设计通用引物分别克隆到山楂‘大黄’和‘徐州大阔’S-RNase基因,命名为DHS3-RNase和XZS4-RNase,并鉴定它们的S基因型分别为S3S3和S4S4。理论预测‘紫珍珠×大黄’后代群体的基因型应该为S1S3和S2S3（1:1）,‘紫珍珠×徐州大货’后代群体的基因型应该为S1S4和S2S4（1:1）。然而根据山楂‘紫珍珠’、‘大黄’和‘徐州大货’的四条S-RNase基因的序列多态性,设计了4对特异性引物S1F/S1R、S2F/S2R、DHS3F/DHS3R和XZS4F/XZS4R,来鉴定‘紫珍珠×大黄’和‘紫珍珠×徐州大货’后代群体的基因型,发现这两个后代群体中均出现了S1S2基因型,与母本‘紫珍珠’基因型一致。因此,我们推测可能是‘紫珍珠’作为母本时,后代种子中存在无融合生殖的现象。
Abstract
shan cha （Crataegus pinnatifida Bge.）shi qiang wei ke （Rosaceae）shan cha shu （Crataegus spp.）guo shu 。zuo wei wo guo chong yao de guo shu chong zhi zi yuan zhi yi ,ji zi jiao bu qin he ji zhi de yan jiu shang wei jian bao dao ,er ‘zi zhen zhu ’zuo wei mu qian fa xian de wei yi shan cha zi jiao qin he xing de chong zhi zi yuan ,wei tan jiu shan cha zi jiao qin bu he xing de fen zi ji zhi ji shan cha shu zhi wu de zi jiao bu qin he ji zhi di gong le chong yao de yan jiu cai liao 。ben yan jiu yi fang mian li yong qiang wei ke guo shu ji yin zu shu ju ku dui he suan mei shu ju jin hang sheng wu xin xi xue fen xi ,fen bie zai 5’UTRhe 3’UTRou yu she ji yin wu ke long ‘zi zhen zhu ’S-RNaseji yin ,bing tong guo NCBIshu ju ku 、Lasergene、MEGA7.0he SWISS MODLEruan jian jin hang tong yuan bi dui 、jie gou fen xi 、ji tong jin hua shu fen xi he dan bai san ji jie gou fen xi ;ling yi fang mian she ji ‘zi zhen zhu ’de zi jiao ji za jiao shou fen shi yan ,tong guo dui zi jiao he za jiao hou dai qun ti de wei chuan fen xi ,lai tao lun ji zi jiao qin he xing tu bian de yuan yin 。jie guo ru xia :（1）cong ‘zi zhen zhu ’zhong ke long chu liang tiao chang du fen bie wei 960 bphe 843 bpde quan chang S-RNaseji yin xu lie ,fen bie ming ming wei ZZZS1-RNasehe ZZZS2-RNase。deng lu dao GeneBankde IDfen bie wei MG385836he MG385837。er zhe jun shu yu RNase T2ji yin jia zu ,tui dao de an ji suan xu lie ju you yu li shu 、ping guo shu S-RNasexiang shi de bao shou jie gou 。ji tong jin hua shu zhong ,ZZZS1-RNasehe ZZZS2-RNaseyu ping guo shu he li shu ju lei zai yi qi ,di shi shan cha shu zi jiao bu qin he lei xing ke neng yu ping guo shu 、li shu wei tong yi lei xing 。（2）‘zi zhen zhu ’ji ji zi jiao hou dai ‘6hao ’jun wei S1S2ji yin xing ,‘zi zhen zhu ’zi hua shou fen qin he zhi shu wei 1.27,biao xian zi jiao qin he ;zi jiao hou dai ‘6hao ’zi hua shou fen qin he zhi shu wei 0.57,biao xian zi jiao bu qin he ,xiang hu shou fen fa xian ‘zi zhen zhu ’gei ‘6hao ’shou fen shi qin he zhi shu wei 0,biao xian zi jiao bu qin he ;er ‘6hao ’zuo fu ben gei ‘zi zhen zhu ’shou fen shi ,qin he zhi shu wei 0.965,biao xian zi jiao qin he 。chu bu pan duan ‘zi zhen zhu ’zi jiao qin he xing tu bian fa sheng zai hua zhu bu fen 。（3）li yong ‘zi zhen zhu ’liang tiao deng wei ji yin de xu lie duo tai xing ,she ji le 2dui te yi xing yin wu dui zi jiao ji za jiao hou dai qun ti Sji yin xing jin hang jian ding ,34ge zi jiao hou dai zhong chu xian le san chong ji yin xing S1S1、S1S2he S2S2（3:26:5）,chu xian le pian fen li xian xiang ;‘zi zhen zhu ×6hao ’34ge za jiao hou dai de san chong ji yin xing S1S1、S1S2he S2S2bi li wei 7:16:11（≈1:2:1）,fu ge meng de er wei chuan gui lv ,zeng shang suo shu ,tui duan ‘zi zhen zhu ’zi jiao qin he xing shi you hua zhu bu fen fei Syin zi tu bian dao zhi 。（4）tong guo she ji tong yong yin wu fen bie ke long dao shan cha ‘da huang ’he ‘xu zhou da kuo ’S-RNaseji yin ,ming ming wei DHS3-RNasehe XZS4-RNase,bing jian ding ta men de Sji yin xing fen bie wei S3S3he S4S4。li lun yu ce ‘zi zhen zhu ×da huang ’hou dai qun ti de ji yin xing ying gai wei S1S3he S2S3（1:1）,‘zi zhen zhu ×xu zhou da huo ’hou dai qun ti de ji yin xing ying gai wei S1S4he S2S4（1:1）。ran er gen ju shan cha ‘zi zhen zhu ’、‘da huang ’he ‘xu zhou da huo ’de si tiao S-RNaseji yin de xu lie duo tai xing ,she ji le 4dui te yi xing yin wu S1F/S1R、S2F/S2R、DHS3F/DHS3Rhe XZS4F/XZS4R,lai jian ding ‘zi zhen zhu ×da huang ’he ‘zi zhen zhu ×xu zhou da huo ’hou dai qun ti de ji yin xing ,fa xian zhe liang ge hou dai qun ti zhong jun chu xian le S1S2ji yin xing ,yu mu ben ‘zi zhen zhu ’ji yin xing yi zhi 。yin ci ,wo men tui ce ke neng shi ‘zi zhen zhu ’zuo wei mu ben shi ,hou dai chong zi zhong cun zai mo rong ge sheng shi de xian xiang 。
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论文作者分别是来自河北科技师范学院的马聚泽，发表于刊物河北科技师范学院2019-07-08论文，是一篇关于山楂论文,自交亲和论文,基因型论文,克隆和分析论文,无融合生殖论文,河北科技师范学院2019-07-08论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自河北科技师范学院2019-07-08论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：山楂论文; 自交亲和论文; 基因型论文; 克隆和分析论文; 无融合生殖论文; 河北科技师范学院2019-07-08论文;