本文主要研究内容
作者左正宇,邵洋,刘杨,卜怡然,王华林,李娜,刘志国（2019）在《虾青素对肝脂代谢与昼夜节律的调节作用》一文中研究指出：研究虾青素对非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)的干预作用及与代谢相关的昼夜节律紊乱的缓解作用。采用高脂/高胆固醇饲喂建立NAFLD小鼠模型和添加虾青素的干预模型。动物实验选用SPF级C57BL/6小鼠,随机分成正常组、高脂模型组和高脂添加虾青素组。采用常规酶联免疫吸附测定法测定血清中甘油三酯、总胆固醇(total cholesterol,TC)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)浓度及谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate transaminase,AST)活力等肝损伤指标,苏木精-伊红染色法观察肝组织形态学变化,荧光定量聚合酶链式反应(real-time quantitative polymerase chain reaction,qPCR)检测与肝脂代谢、胆固醇代谢、昼夜节律相关的基因表达及昼夜节律变化。结果显示:与正常组相比,高脂模型组小鼠体质量、肝脏指数、肥胖指数均高度显著增加(P<0.001),虾青素干预后肝脏指数、肥胖指数均极显著降低(P<0.01)。高脂饲喂导致小鼠血脂水平升高(HDL-C除外),尤其是LDL-C浓度升高明显,而虾青素干预可以有效降低TC和LDL-C浓度,并升高HDL-C浓度,改善脂质的代谢。高脂/高胆固醇饲喂会高度显著升高小鼠血清ALT、AST活力(P<0.001),造成肝损伤。与高脂模型组相比,虾青素干预可极显著降低小鼠ALT、AST活力(P<0.01),缓解肝脏损伤,并明显改善肝脏脂质代谢关键酶的基因表达,包括脂肪酸合成酶和胆固醇合成关键酶羟甲基戊二酸单酰辅酶A还原酶。同时,虾青素干预也增加了胆固醇7α-羟化酶的表达量,促进胆固醇氧化。虾青素调节上述基因的表达具有昼夜节律性,能够缓解或修正由高脂导致的生物钟节律相关因子时钟昼夜节律调节器及脑和肌肉芳香烃受体核转运体样蛋白1以及肝脏脂质代谢关键基因昼夜节律表达的紊乱。本研究表明虾青素具有改善高脂所致肝代谢紊乱的节律性调节作用。
Abstract
yan jiu ha qing su dui fei jiu jing xing zhi fang xing gan bing (non-alcoholic fatty liver disease,NAFLD)de gan yu zuo yong ji yu dai xie xiang guan de zhou ye jie lv wen luan de huan jie zuo yong 。cai yong gao zhi /gao dan gu chun si wei jian li NAFLDxiao shu mo xing he tian jia ha qing su de gan yu mo xing 。dong wu shi yan shua yong SPFji C57BL/6xiao shu ,sui ji fen cheng zheng chang zu 、gao zhi mo xing zu he gao zhi tian jia ha qing su zu 。cai yong chang gui mei lian mian yi xi fu ce ding fa ce ding xie qing zhong gan you san zhi 、zong dan gu chun (total cholesterol,TC)、gao mi du zhi dan bai dan gu chun (high density lipoprotein cholesterol,HDL-C)、di mi du zhi dan bai dan gu chun (low density lipoprotein cholesterol,LDL-C)nong du ji gu bing zhuai an mei (alanine aminotransferase,ALT)、gu cao zhuai an mei (aspartate transaminase,AST)huo li deng gan sun shang zhi biao ,su mu jing -yi gong ran se fa guan cha gan zu zhi xing tai xue bian hua ,ying guang ding liang ju ge mei lian shi fan ying (real-time quantitative polymerase chain reaction,qPCR)jian ce yu gan zhi dai xie 、dan gu chun dai xie 、zhou ye jie lv xiang guan de ji yin biao da ji zhou ye jie lv bian hua 。jie guo xian shi :yu zheng chang zu xiang bi ,gao zhi mo xing zu xiao shu ti zhi liang 、gan zang zhi shu 、fei pan zhi shu jun gao du xian zhe zeng jia (P<0.001),ha qing su gan yu hou gan zang zhi shu 、fei pan zhi shu jun ji xian zhe jiang di (P<0.01)。gao zhi si wei dao zhi xiao shu xie zhi shui ping sheng gao (HDL-Cchu wai ),you ji shi LDL-Cnong du sheng gao ming xian ,er ha qing su gan yu ke yi you xiao jiang di TChe LDL-Cnong du ,bing sheng gao HDL-Cnong du ,gai shan zhi zhi de dai xie 。gao zhi /gao dan gu chun si wei hui gao du xian zhe sheng gao xiao shu xie qing ALT、ASThuo li (P<0.001),zao cheng gan sun shang 。yu gao zhi mo xing zu xiang bi ,ha qing su gan yu ke ji xian zhe jiang di xiao shu ALT、ASThuo li (P<0.01),huan jie gan zang sun shang ,bing ming xian gai shan gan zang zhi zhi dai xie guan jian mei de ji yin biao da ,bao gua zhi fang suan ge cheng mei he dan gu chun ge cheng guan jian mei qiang jia ji wu er suan chan xian fu mei Ahai yuan mei 。tong shi ,ha qing su gan yu ye zeng jia le dan gu chun 7α-qiang hua mei de biao da liang ,cu jin dan gu chun yang hua 。ha qing su diao jie shang shu ji yin de biao da ju you zhou ye jie lv xing ,neng gou huan jie huo xiu zheng you gao zhi dao zhi de sheng wu zhong jie lv xiang guan yin zi shi zhong zhou ye jie lv diao jie qi ji nao he ji rou fang xiang ting shou ti he zhuai yun ti yang dan bai 1yi ji gan zang zhi zhi dai xie guan jian ji yin zhou ye jie lv biao da de wen luan 。ben yan jiu biao ming ha qing su ju you gai shan gao zhi suo zhi gan dai xie wen luan de jie lv xing diao jie zuo yong 。
论文参考文献
论文详细介绍
论文作者分别是来自食品科学的左正宇,邵洋,刘杨,卜怡然,王华林,李娜,刘志国，发表于刊物食品科学2019年03期论文，是一篇关于虾青素论文,非酒精性脂肪性肝病论文,昼夜节律论文,食品科学2019年03期论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自食品科学2019年03期论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：虾青素论文; 非酒精性脂肪性肝病论文; 昼夜节律论文; 食品科学2019年03期论文;