本文主要研究内容
作者张鑫（2019）在《两种树形对苹果幼树生长发育的影响及苹果MCU基因家族的鉴定》一文中研究指出：本试验以3年生‘烟富3号’高纺锤形和Y字形苹果幼树为材料,结合两种树形萌芽率、成花率、主干和主枝新梢长度、主干和主枝新梢粗度、主干和主枝新梢节间长度、主干短枝顶芽碳氮含量、主干短枝顶芽C/N、主干短枝顶芽内源激素含量及主干短枝顶芽内成花相关基因表达量的测定结果,对两种树形苹果幼树生长及成花的影响进行综合评价。本文还鉴定了5个苹果MCU基因,为进一步这研究这些MCU基因的功能奠定了基础。结果如下:1、高纺锤形苹果幼树长枝比例、主干新稍年生长量和主干新稍粗度显著高于Y字形,Y字形苹果幼树在主干的成花率和短枝比例显著高于高纺锤形。而两种树形主干和主枝上的萌芽率、主枝上的成花率、中枝比例、主枝新稍粗度、主枝新稍年生长量、主干新梢节间长度、主枝新梢节间长度无显著差异。整体上Y字形苹果幼树主干短枝顶芽C/N比高于高纺锤形。Y字形苹果树主干短枝顶芽内,ABA和ZT的含量在花后50d都高于高纺锤形,而在高纺锤形主干短枝顶芽内,GA3和IAA的含量在花后50d和70d都显著高于Y字形。Y字形主干短枝顶芽内MdFT的表达量在花后50d比高纺锤形显著高46.47%。高纺锤形主干短枝顶芽内MdAP1表达量在花后70d明显低于Y字形。Y字形主干短枝顶芽内MdLFY的表达量在花后30d、50d和70d比高纺锤形高分别显著高出40.25%、20.10%和23.53%。在花后50d和90d,Y字形主干短枝顶芽内MdSOC1的表达量比高纺锤形分别显著高出33.33%和96.30%。本文初步探讨高纺锤形和Y字形苹果幼树的差异比较,认为Y字形苹果幼树较高纺锤形易形成结果短枝,为筛选易成花树形提供依据。然而,未能深入研究两种树形在大小年相关指标的差异,以及幼树生长环境对成花的作用。2、本研究还鉴定了苹果MCU基因家族候选成员。该家族有5个MdMCU基因,含有2-5个外显子,1-4个内含子,7个共同的保守基序,且聚为2个亚家族。五个基因均含有有关光响应和脱落酸诱导有关的顺式作用调控元件。另外,基于公开的基因表达数据表明,该家族成员在不同组织中均广泛表达。这些都为将来进一步研究MdMCUs基因的功能奠定了基础。
Abstract
ben shi yan yi 3nian sheng ‘yan fu 3hao ’gao fang chui xing he Yzi xing ping guo you shu wei cai liao ,jie ge liang chong shu xing meng ya lv 、cheng hua lv 、zhu gan he zhu zhi xin sao chang du 、zhu gan he zhu zhi xin sao cu du 、zhu gan he zhu zhi xin sao jie jian chang du 、zhu gan duan zhi ding ya tan dan han liang 、zhu gan duan zhi ding ya C/N、zhu gan duan zhi ding ya nei yuan ji su han liang ji zhu gan duan zhi ding ya nei cheng hua xiang guan ji yin biao da liang de ce ding jie guo ,dui liang chong shu xing ping guo you shu sheng chang ji cheng hua de ying xiang jin hang zeng ge ping jia 。ben wen hai jian ding le 5ge ping guo MCUji yin ,wei jin yi bu zhe yan jiu zhe xie MCUji yin de gong neng dian ding le ji chu 。jie guo ru xia :1、gao fang chui xing ping guo you shu chang zhi bi li 、zhu gan xin shao nian sheng chang liang he zhu gan xin shao cu du xian zhe gao yu Yzi xing ,Yzi xing ping guo you shu zai zhu gan de cheng hua lv he duan zhi bi li xian zhe gao yu gao fang chui xing 。er liang chong shu xing zhu gan he zhu zhi shang de meng ya lv 、zhu zhi shang de cheng hua lv 、zhong zhi bi li 、zhu zhi xin shao cu du 、zhu zhi xin shao nian sheng chang liang 、zhu gan xin sao jie jian chang du 、zhu zhi xin sao jie jian chang du mo xian zhe cha yi 。zheng ti shang Yzi xing ping guo you shu zhu gan duan zhi ding ya C/Nbi gao yu gao fang chui xing 。Yzi xing ping guo shu zhu gan duan zhi ding ya nei ,ABAhe ZTde han liang zai hua hou 50ddou gao yu gao fang chui xing ,er zai gao fang chui xing zhu gan duan zhi ding ya nei ,GA3he IAAde han liang zai hua hou 50dhe 70ddou xian zhe gao yu Yzi xing 。Yzi xing zhu gan duan zhi ding ya nei MdFTde biao da liang zai hua hou 50dbi gao fang chui xing xian zhe gao 46.47%。gao fang chui xing zhu gan duan zhi ding ya nei MdAP1biao da liang zai hua hou 70dming xian di yu Yzi xing 。Yzi xing zhu gan duan zhi ding ya nei MdLFYde biao da liang zai hua hou 30d、50dhe 70dbi gao fang chui xing gao fen bie xian zhe gao chu 40.25%、20.10%he 23.53%。zai hua hou 50dhe 90d,Yzi xing zhu gan duan zhi ding ya nei MdSOC1de biao da liang bi gao fang chui xing fen bie xian zhe gao chu 33.33%he 96.30%。ben wen chu bu tan tao gao fang chui xing he Yzi xing ping guo you shu de cha yi bi jiao ,ren wei Yzi xing ping guo you shu jiao gao fang chui xing yi xing cheng jie guo duan zhi ,wei shai shua yi cheng hua shu xing di gong yi ju 。ran er ,wei neng shen ru yan jiu liang chong shu xing zai da xiao nian xiang guan zhi biao de cha yi ,yi ji you shu sheng chang huan jing dui cheng hua de zuo yong 。2、ben yan jiu hai jian ding le ping guo MCUji yin jia zu hou shua cheng yuan 。gai jia zu you 5ge MdMCUji yin ,han you 2-5ge wai xian zi ,1-4ge nei han zi ,7ge gong tong de bao shou ji xu ,ju ju wei 2ge ya jia zu 。wu ge ji yin jun han you you guan guang xiang ying he tuo la suan you dao you guan de shun shi zuo yong diao kong yuan jian 。ling wai ,ji yu gong kai de ji yin biao da shu ju biao ming ,gai jia zu cheng yuan zai bu tong zu zhi zhong jun an fan biao da 。zhe xie dou wei jiang lai jin yi bu yan jiu MdMCUsji yin de gong neng dian ding le ji chu 。
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论文详细介绍
论文作者分别是来自西北农林科技大学的张鑫，发表于刊物西北农林科技大学2019-07-11论文，是一篇关于苹果论文,幼树论文,树形论文,生长发育论文,基因论文,西北农林科技大学2019-07-11论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自西北农林科技大学2019-07-11论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。