本文主要研究内容
作者章旖旎（2019）在《Klebsiella sp. 2e对粗甘油不同杂质耐受性及转化1，3-丙二醇关键酶研究》一文中研究指出：本研究以一株筛选出的粗甘油耐受型菌株Klebsiella sp.2e为研究对象,通过对细菌各种生理生化指标进行检测,及对KCl、K2SO4、甲醇、油酸四种杂质发酵液与粗甘油和纯甘油发酵液中菌株生长量、甘油消耗量和1,3-丙二醇的产率检测,确定不同杂质及其不同浓度参数对菌株生长发酵合成1,3-丙二醇的影响,了解反应发生过程与趋势;同时,结合甘油转化过程中三种关键酶:即甘油脱氢酶(GDH)、甘油脱水酶(GDHt)及1,3-丙二醇氧化酶(PDOR)的活性分析,探究不同杂质对酶活性的影响,进一步探究Klebsiella sp.2e对粗甘油的耐受性特点。结果表明:1.用不同分离筛选培养基培养测定Klebsiella sp.2e菌株生长量时,低浓度的KCl杂质对菌株生长发酵没有影响,KC1与培养基质液比高于5%时会对菌株生长有抑制作用,可见过量的无机盐离子会影响细菌外的渗透压从而影响其生长繁殖;低于10 g/L的甲醇溶液对菌株生长没有影响,与在纯甘油发酵液中生长情况一致;而K2SO4、油酸即使是少量也会抑制菌株的生长,证明-SO4O42-与有机酸对Klebsiella sp.2e的生长影响较大,在发酵前可对粗甘油进行抽样检测,以预处理去除-SO42-与有机酸。2.在用不同的分离筛选培养基发酵培养Klebsiella sp.2e的过程中,发酵液中甘油消耗量与1,3-丙二醇生成量成正比,甘油转化效率与菌株生长量有关,浓度低于5%的KCl杂质能促进菌株发酵生产1,3-丙二醇,高于这个值则开始出现抑制作用;低于10g/L的甲醇杂质对菌株的发酵作用没有影响,1,3-丙二醇产率接近纯甘油发酵产率;在纯甘油培养基中甘油转化1,3-PDO转化率高达46.4%,超过一般甘油转化菌株,而在粗甘油培养基中转化率在36 h后仅为36.8%,可见粗甘油中的一些杂质虽然可以促进菌株生长,但却抑制了甘油反应转化成1,3-丙二醇。3.在酶活性分析中,甘油脱氢酶(GDH)、甘油脱水酶(GDHt)及1,3-丙二醇氧化还原酶(PDOR)在对数期活性较大,一般高于稳定期与衰退期,此时菌株活性最大,反应时碳源主要流向还原途径,使发酵前期能保持较高的3-羟基丙醛浓度,较少的乙酸等反应副产物,促进甘油转化生成1,3-丙二醇。但在发酵后期会生成一些性质相似的中间产物的代谢酶,导致酶活性测定值会增高,而实际上三种甘油转化酶的酶活性降低。4.利用Klebsiella sp.2e直接发酵粗甘油废料,该废料是以光皮树油为原料生产生物柴油时产生的副产物,36h后1,3-丙二醇产率接近于添加同等质量纯甘油的发酵液,菌株Klebsiella sp.2e对粗甘油有良好的耐受性。
Abstract
ben yan jiu yi yi zhu shai shua chu de cu gan you nai shou xing jun zhu Klebsiella sp.2ewei yan jiu dui xiang ,tong guo dui xi jun ge chong sheng li sheng hua zhi biao jin hang jian ce ,ji dui KCl、K2SO4、jia chun 、you suan si chong za zhi fa jiao ye yu cu gan you he chun gan you fa jiao ye zhong jun zhu sheng chang liang 、gan you xiao hao liang he 1,3-bing er chun de chan lv jian ce ,que ding bu tong za zhi ji ji bu tong nong du can shu dui jun zhu sheng chang fa jiao ge cheng 1,3-bing er chun de ying xiang ,le jie fan ying fa sheng guo cheng yu qu shi ;tong shi ,jie ge gan you zhuai hua guo cheng zhong san chong guan jian mei :ji gan you tuo qing mei (GDH)、gan you tuo shui mei (GDHt)ji 1,3-bing er chun yang hua mei (PDOR)de huo xing fen xi ,tan jiu bu tong za zhi dui mei huo xing de ying xiang ,jin yi bu tan jiu Klebsiella sp.2edui cu gan you de nai shou xing te dian 。jie guo biao ming :1.yong bu tong fen li shai shua pei yang ji pei yang ce ding Klebsiella sp.2ejun zhu sheng chang liang shi ,di nong du de KClza zhi dui jun zhu sheng chang fa jiao mei you ying xiang ,KC1yu pei yang ji zhi ye bi gao yu 5%shi hui dui jun zhu sheng chang you yi zhi zuo yong ,ke jian guo liang de mo ji yan li zi hui ying xiang xi jun wai de shen tou ya cong er ying xiang ji sheng chang fan shi ;di yu 10 g/Lde jia chun rong ye dui jun zhu sheng chang mei you ying xiang ,yu zai chun gan you fa jiao ye zhong sheng chang qing kuang yi zhi ;er K2SO4、you suan ji shi shi shao liang ye hui yi zhi jun zhu de sheng chang ,zheng ming -SO4O42-yu you ji suan dui Klebsiella sp.2ede sheng chang ying xiang jiao da ,zai fa jiao qian ke dui cu gan you jin hang chou yang jian ce ,yi yu chu li qu chu -SO42-yu you ji suan 。2.zai yong bu tong de fen li shai shua pei yang ji fa jiao pei yang Klebsiella sp.2ede guo cheng zhong ,fa jiao ye zhong gan you xiao hao liang yu 1,3-bing er chun sheng cheng liang cheng zheng bi ,gan you zhuai hua xiao lv yu jun zhu sheng chang liang you guan ,nong du di yu 5%de KClza zhi neng cu jin jun zhu fa jiao sheng chan 1,3-bing er chun ,gao yu zhe ge zhi ze kai shi chu xian yi zhi zuo yong ;di yu 10g/Lde jia chun za zhi dui jun zhu de fa jiao zuo yong mei you ying xiang ,1,3-bing er chun chan lv jie jin chun gan you fa jiao chan lv ;zai chun gan you pei yang ji zhong gan you zhuai hua 1,3-PDOzhuai hua lv gao da 46.4%,chao guo yi ban gan you zhuai hua jun zhu ,er zai cu gan you pei yang ji zhong zhuai hua lv zai 36 hhou jin wei 36.8%,ke jian cu gan you zhong de yi xie za zhi sui ran ke yi cu jin jun zhu sheng chang ,dan que yi zhi le gan you fan ying zhuai hua cheng 1,3-bing er chun 。3.zai mei huo xing fen xi zhong ,gan you tuo qing mei (GDH)、gan you tuo shui mei (GDHt)ji 1,3-bing er chun yang hua hai yuan mei (PDOR)zai dui shu ji huo xing jiao da ,yi ban gao yu wen ding ji yu cui tui ji ,ci shi jun zhu huo xing zui da ,fan ying shi tan yuan zhu yao liu xiang hai yuan tu jing ,shi fa jiao qian ji neng bao chi jiao gao de 3-qiang ji bing quan nong du ,jiao shao de yi suan deng fan ying fu chan wu ,cu jin gan you zhuai hua sheng cheng 1,3-bing er chun 。dan zai fa jiao hou ji hui sheng cheng yi xie xing zhi xiang shi de zhong jian chan wu de dai xie mei ,dao zhi mei huo xing ce ding zhi hui zeng gao ,er shi ji shang san chong gan you zhuai hua mei de mei huo xing jiang di 。4.li yong Klebsiella sp.2ezhi jie fa jiao cu gan you fei liao ,gai fei liao shi yi guang pi shu you wei yuan liao sheng chan sheng wu chai you shi chan sheng de fu chan wu ,36hhou 1,3-bing er chun chan lv jie jin yu tian jia tong deng zhi liang chun gan you de fa jiao ye ,jun zhu Klebsiella sp.2edui cu gan you you liang hao de nai shou xing 。
论文参考文献
论文详细介绍
论文作者分别是来自中南林业科技大学的章旖旎，发表于刊物中南林业科技大学2019-09-29论文，是一篇关于粗甘油论文,酶活性论文,丙二醇论文,耐受性论文,中南林业科技大学2019-09-29论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自中南林业科技大学2019-09-29论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：粗甘油论文; 酶活性论文; 丙二醇论文; 耐受性论文; 中南林业科技大学2019-09-29论文;