本文主要研究内容
作者刘芸芸（2019）在《桦褐孔菌、忧遁草等中药化学成分分离纯化方法研究》一文中研究指出：由于中药所含成分复杂,有效成分含量低,而限制了其利用和发展,因此建立方便有效的分离纯化方法对于促进中药现代化研究及应用尤为重要。本文通过对桦褐孔菌等中草药化学成分分离纯化方法的研究,建立了桦褐孔菌、忧遁草、通关藤、黑老虎及陈皮中化学成分的高效液相色谱（HPLC）分析方法,并采用大孔吸附树脂法（MAR）对其中化学成分的纯化工艺进行了研究,从而建立了方便、高效的纯化方法。1.桦褐孔菌化学成分分离纯化方法研究对桦褐孔菌中的化学成分进行了提取工艺的优化,最佳提取工艺为:用95%乙醇（料液比1:20）回流提取2次,每次0.5 h。采用大孔吸附树脂法对桦褐孔菌三萜类成分的纯化工艺进行研究,以桦褐孔菌中三萜成分的量为指标,比较不同极性大孔树脂对桦褐孔菌三萜的吸附和解吸附性能,然后优化树脂纯化的工艺参数。结果显示,D101型大孔树脂具有较好的吸附和解吸性能,吸附行为更符合Langmuir模型。最佳工艺条件为:5 BV 4 mg·mL-1粗提物溶液上样后,用10 BV 40%乙醇和7 BV 95%乙醇以2 mL·min-1的流速分别进行除杂和纯化,经树脂纯化后的桦褐孔菌三萜含量由25.68%提高至82.00%,确定了本实验所建立的方法能有效纯化桦褐孔菌三萜类物质。2.忧遁草化学成分分离纯化方法研究采用大孔吸附树脂法对忧遁草黄酮类成分的纯化工艺进行研究。以忧遁草黄酮成分的量为指标,比较了不同极性大孔树脂对忧遁草黄酮的吸附和解吸附性能,然后优化树脂的工艺参数。结果显示,HPD-100型大孔树脂具有较好的吸附和解吸性能,吸附行为更符合Langmuir模型。最佳工艺条件为:6 BV 10 mg·mL-1粗提物溶液上样后,用10 BV水和7 BV 40%乙醇以4 mL·min-1的流速分别进行除杂和纯化,经树脂纯化后的忧遁草黄酮含量由61.28%提高至91.74%,确定了本实验所建立的方法能有效纯化忧遁草黄酮类物质。3.通关藤化学成分分离纯化方法研究采用大孔吸附树脂法对通关藤C21甾体皂苷类成分的纯化工艺进行研究。以C21甾体皂苷成分的量为指标,比较了不同极性大孔树脂对通关藤C21甾体皂苷的吸附和解吸附性能,然后优化树脂的工艺参数。结果显示,AB-8型大孔树脂具有较好的吸附和解吸性能,吸附行为更符合Langmuir模型。最佳工艺条件为:8 BV 10 mg·mL-1粗提物溶液上样后,用10 BV 20%乙醇和7 BV 80%乙醇以4 mL·min-1的流速分别进行除杂和纯化,经树脂纯化后的通关藤C21甾体皂苷含量由42.18%提高至88.59%,确定了本实验所建立的方法能有效纯化通关藤C21甾体皂苷类物质。4.黑老虎化学成分分离纯化方法研究采用大孔吸附树脂法对黑老虎化学成分的纯化工艺进行研究,比较了不同极性大孔树脂的吸附和解吸附性能,然后优化树脂的工艺参数。结果显示,HPD-400型大孔树脂具有较好的吸附和解吸性能,吸附行为更符合Langmuir模型。最佳工艺条件为:6 BV 10 mg·mL-1粗提物溶液上样后,用10 BV 20%乙醇、80 BV 40%乙醇和5 BV 95%乙醇以4 mL·min-1的流速分别除去杂质、纯化高极性和低极性成分,经树脂纯化后的黑老虎高极性含量由20.87%提高至88.41%,低极性含量由11.49%提高至81.05%,确定了本实验所建立的方法能有效纯化黑老虎化学成分。5.陈皮化学成分分离纯化方法研究采用大孔吸附树脂法对陈皮黄酮类成分的纯化工艺进行研究。以陈皮黄酮成分的量为指标,比较不同极性的大孔树脂对陈皮黄酮的吸附和解吸附性能,然后优化树脂的工艺参数。结果显示,HPD-400型大孔树脂具有较好的吸附和解吸性能,吸附行为更符合Langmuir模型。最佳工艺条件为:5 BV 80 mg·mL-1粗提物溶液上样后,用10BV水和8 BV 80%乙醇以4 mL·min-1的流速分别进行除杂和纯化,经树脂纯化后的陈皮黄酮含量由42.18%提高至88.59%,确定了本实验所建立的方法能有效纯化陈皮黄酮类物质。
Abstract
you yu zhong yao suo han cheng fen fu za ,you xiao cheng fen han liang di ,er xian zhi le ji li yong he fa zhan ,yin ci jian li fang bian you xiao de fen li chun hua fang fa dui yu cu jin zhong yao xian dai hua yan jiu ji ying yong you wei chong yao 。ben wen tong guo dui hua he kong jun deng zhong cao yao hua xue cheng fen fen li chun hua fang fa de yan jiu ,jian li le hua he kong jun 、you dun cao 、tong guan teng 、hei lao hu ji chen pi zhong hua xue cheng fen de gao xiao ye xiang se pu （HPLC）fen xi fang fa ,bing cai yong da kong xi fu shu zhi fa （MAR）dui ji zhong hua xue cheng fen de chun hua gong yi jin hang le yan jiu ,cong er jian li le fang bian 、gao xiao de chun hua fang fa 。1.hua he kong jun hua xue cheng fen fen li chun hua fang fa yan jiu dui hua he kong jun zhong de hua xue cheng fen jin hang le di qu gong yi de you hua ,zui jia di qu gong yi wei :yong 95%yi chun （liao ye bi 1:20）hui liu di qu 2ci ,mei ci 0.5 h。cai yong da kong xi fu shu zhi fa dui hua he kong jun san tie lei cheng fen de chun hua gong yi jin hang yan jiu ,yi hua he kong jun zhong san tie cheng fen de liang wei zhi biao ,bi jiao bu tong ji xing da kong shu zhi dui hua he kong jun san tie de xi fu he jie xi fu xing neng ,ran hou you hua shu zhi chun hua de gong yi can shu 。jie guo xian shi ,D101xing da kong shu zhi ju you jiao hao de xi fu he jie xi xing neng ,xi fu hang wei geng fu ge Langmuirmo xing 。zui jia gong yi tiao jian wei :5 BV 4 mg·mL-1cu di wu rong ye shang yang hou ,yong 10 BV 40%yi chun he 7 BV 95%yi chun yi 2 mL·min-1de liu su fen bie jin hang chu za he chun hua ,jing shu zhi chun hua hou de hua he kong jun san tie han liang you 25.68%di gao zhi 82.00%,que ding le ben shi yan suo jian li de fang fa neng you xiao chun hua hua he kong jun san tie lei wu zhi 。2.you dun cao hua xue cheng fen fen li chun hua fang fa yan jiu cai yong da kong xi fu shu zhi fa dui you dun cao huang tong lei cheng fen de chun hua gong yi jin hang yan jiu 。yi you dun cao huang tong cheng fen de liang wei zhi biao ,bi jiao le bu tong ji xing da kong shu zhi dui you dun cao huang tong de xi fu he jie xi fu xing neng ,ran hou you hua shu zhi de gong yi can shu 。jie guo xian shi ,HPD-100xing da kong shu zhi ju you jiao hao de xi fu he jie xi xing neng ,xi fu hang wei geng fu ge Langmuirmo xing 。zui jia gong yi tiao jian wei :6 BV 10 mg·mL-1cu di wu rong ye shang yang hou ,yong 10 BVshui he 7 BV 40%yi chun yi 4 mL·min-1de liu su fen bie jin hang chu za he chun hua ,jing shu zhi chun hua hou de you dun cao huang tong han liang you 61.28%di gao zhi 91.74%,que ding le ben shi yan suo jian li de fang fa neng you xiao chun hua you dun cao huang tong lei wu zhi 。3.tong guan teng hua xue cheng fen fen li chun hua fang fa yan jiu cai yong da kong xi fu shu zhi fa dui tong guan teng C21zai ti zao gan lei cheng fen de chun hua gong yi jin hang yan jiu 。yi C21zai ti zao gan cheng fen de liang wei zhi biao ,bi jiao le bu tong ji xing da kong shu zhi dui tong guan teng C21zai ti zao gan de xi fu he jie xi fu xing neng ,ran hou you hua shu zhi de gong yi can shu 。jie guo xian shi ,AB-8xing da kong shu zhi ju you jiao hao de xi fu he jie xi xing neng ,xi fu hang wei geng fu ge Langmuirmo xing 。zui jia gong yi tiao jian wei :8 BV 10 mg·mL-1cu di wu rong ye shang yang hou ,yong 10 BV 20%yi chun he 7 BV 80%yi chun yi 4 mL·min-1de liu su fen bie jin hang chu za he chun hua ,jing shu zhi chun hua hou de tong guan teng C21zai ti zao gan han liang you 42.18%di gao zhi 88.59%,que ding le ben shi yan suo jian li de fang fa neng you xiao chun hua tong guan teng C21zai ti zao gan lei wu zhi 。4.hei lao hu hua xue cheng fen fen li chun hua fang fa yan jiu cai yong da kong xi fu shu zhi fa dui hei lao hu hua xue cheng fen de chun hua gong yi jin hang yan jiu ,bi jiao le bu tong ji xing da kong shu zhi de xi fu he jie xi fu xing neng ,ran hou you hua shu zhi de gong yi can shu 。jie guo xian shi ,HPD-400xing da kong shu zhi ju you jiao hao de xi fu he jie xi xing neng ,xi fu hang wei geng fu ge Langmuirmo xing 。zui jia gong yi tiao jian wei :6 BV 10 mg·mL-1cu di wu rong ye shang yang hou ,yong 10 BV 20%yi chun 、80 BV 40%yi chun he 5 BV 95%yi chun yi 4 mL·min-1de liu su fen bie chu qu za zhi 、chun hua gao ji xing he di ji xing cheng fen ,jing shu zhi chun hua hou de hei lao hu gao ji xing han liang you 20.87%di gao zhi 88.41%,di ji xing han liang you 11.49%di gao zhi 81.05%,que ding le ben shi yan suo jian li de fang fa neng you xiao chun hua hei lao hu hua xue cheng fen 。5.chen pi hua xue cheng fen fen li chun hua fang fa yan jiu cai yong da kong xi fu shu zhi fa dui chen pi huang tong lei cheng fen de chun hua gong yi jin hang yan jiu 。yi chen pi huang tong cheng fen de liang wei zhi biao ,bi jiao bu tong ji xing de da kong shu zhi dui chen pi huang tong de xi fu he jie xi fu xing neng ,ran hou you hua shu zhi de gong yi can shu 。jie guo xian shi ,HPD-400xing da kong shu zhi ju you jiao hao de xi fu he jie xi xing neng ,xi fu hang wei geng fu ge Langmuirmo xing 。zui jia gong yi tiao jian wei :5 BV 80 mg·mL-1cu di wu rong ye shang yang hou ,yong 10BVshui he 8 BV 80%yi chun yi 4 mL·min-1de liu su fen bie jin hang chu za he chun hua ,jing shu zhi chun hua hou de chen pi huang tong han liang you 42.18%di gao zhi 88.59%,que ding le ben shi yan suo jian li de fang fa neng you xiao chun hua chen pi huang tong lei wu zhi 。
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论文详细介绍
论文作者分别是来自聊城大学的刘芸芸，发表于刊物聊城大学2019-09-30论文，是一篇关于大孔吸附树脂论文,纯化工艺论文,桦褐孔菌论文,忧遁草论文,通关藤论文,黑老虎论文,陈皮论文,聊城大学2019-09-30论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自聊城大学2019-09-30论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：大孔吸附树脂论文; 纯化工艺论文; 桦褐孔菌论文; 忧遁草论文; 通关藤论文; 黑老虎论文; 陈皮论文; 聊城大学2019-09-30论文;