本文主要研究内容
作者曹宁（2019）在《转录组分析BR、JA和SA在玉米褪绿斑驳病毒侵染过程中的作用》一文中研究指出：玉米(Zea mays L.)是世界上种植最广泛的作物之一,而近年来玉米致死性坏死病(maize lethal necrosis,MLN)给玉米生产带来了毁灭性打击。玉米褪绿斑驳病毒(maize chlorotic mottle virus,MCMV)是MLN的必需病原,单独感染引起玉米叶片失绿、斑驳等症状,是威胁玉米产业发展的重大隐患。筛选在玉米-MCMV互作中起关键作用的激素途径以及鉴定该途径在MCMV侵染中的作用,对于深入解析MCMV致病机制和提出抗病毒策略具有重要意义。为了解玉米-MCMV的互作情况,本实验借助高通量测序技术从转录组水平上对未感染MCMV和感染MCMV玉米植株进行了基因表达分析。我们从实验组和对照组样品中共获得21,939个基因,从上述基因中搜索差异表达基因(differentially expressed genes,DEGs),共检测出206个DEGs,其中有86个基因较对照组显著上调,120个基因显著下调。对这些DEGs进行GO和KEGG pathway富集分析,分别得到107条显著富集的GO terms和8条显著富集的途径。转录组数据显示,MCMV侵染上调了第一片系统侵染叶(first systematically infected leaf,SL1)中油菜素类固醇(brassinosteroid,BR)合成相关基因的表达。RT-qPCR表明,MCMV侵染后玉米SL1中BR相关基因的表达上调;酶联免疫吸附分析(enzyme-linked immunosorbent assay,ELISA)证明,MCMV侵染后玉米SL1中BR的含量增加。叶面喷施BR诱导了玉米对MCMV的感病性,该过程伴随着一氧化氮(nitric oxide,NO)的积累。然而,当外施NO清除剂时,BR对玉米感病性的诱导消失,这表明BR诱导的玉米对MCMV的感病性依赖于NO积累。以上研究结果表明,BR通过依赖NO的方式诱导玉米对MCMV的感病性。转录组数据表明,接种MCMV的玉米中茉莉酸(jasmonic acid,JA)合成相关以及水杨酸(salicylic acid,SA)合成相关的基因表达上调,RT-qPCR证实了这一结果。高效液相色谱分析(high performance liquid chromatography,HPLC)表明,MCMV侵染后玉米SL1中JA和SA的含量均显著增加。叶面喷施JA或SA均能增强玉米对MCMV的抗病性。然而,同时喷施JA和SA时,玉米植株对MCMV抗性的诱导程度弱于单独喷施JA或SA,这表明JA、SA在介导玉米对MCMV抗病性的过程中存在明显的拮抗作用。此外,我们还发现BR能够在玉米感染MCMV过程中的激活SA,主要表现为叶面喷施BR后,SA途径的标记基因ZmPR1a以及SA合成基因ZmPALs的表达量均显著升高。综合MCMV侵染玉米后BR、JA和SA的响应以及这几种激素对MCMV积累的影响,我们提出了一种模型:MCMV的侵染诱导了玉米叶片中BR的积累,BR浓度的升高一方面促进了MCMV的积累,另一方面激活了植物体内的抗病毒激素信号SA,SA积累到一定水平便开始制衡MCMV,该过程需要NO参与。此外,MCMV侵染也诱导了玉米叶片中JA的积累,JA能诱导玉米对MCMV的抗性,但JA与SA存在显著的拮抗作用。复杂的激素体系维持了植株与病毒的势均力敌,本研究从激素角度解析了激素及其信号转导途径在MCMV侵染中的功能,为研究MCMV的致病机制提供了理论依据。
Abstract
yu mi (Zea mays L.)shi shi jie shang chong zhi zui an fan de zuo wu zhi yi ,er jin nian lai yu mi zhi si xing huai si bing (maize lethal necrosis,MLN)gei yu mi sheng chan dai lai le hui mie xing da ji 。yu mi tui lu ban bo bing du (maize chlorotic mottle virus,MCMV)shi MLNde bi xu bing yuan ,chan du gan ran yin qi yu mi xie pian shi lu 、ban bo deng zheng zhuang ,shi wei xie yu mi chan ye fa zhan de chong da yin huan 。shai shua zai yu mi -MCMVhu zuo zhong qi guan jian zuo yong de ji su tu jing yi ji jian ding gai tu jing zai MCMVqin ran zhong de zuo yong ,dui yu shen ru jie xi MCMVzhi bing ji zhi he di chu kang bing du ce lve ju you chong yao yi yi 。wei le jie yu mi -MCMVde hu zuo qing kuang ,ben shi yan jie zhu gao tong liang ce xu ji shu cong zhuai lu zu shui ping shang dui wei gan ran MCMVhe gan ran MCMVyu mi zhi zhu jin hang le ji yin biao da fen xi 。wo men cong shi yan zu he dui zhao zu yang pin zhong gong huo de 21,939ge ji yin ,cong shang shu ji yin zhong sou suo cha yi biao da ji yin (differentially expressed genes,DEGs),gong jian ce chu 206ge DEGs,ji zhong you 86ge ji yin jiao dui zhao zu xian zhe shang diao ,120ge ji yin xian zhe xia diao 。dui zhe xie DEGsjin hang GOhe KEGG pathwayfu ji fen xi ,fen bie de dao 107tiao xian zhe fu ji de GO termshe 8tiao xian zhe fu ji de tu jing 。zhuai lu zu shu ju xian shi ,MCMVqin ran shang diao le di yi pian ji tong qin ran xie (first systematically infected leaf,SL1)zhong you cai su lei gu chun (brassinosteroid,BR)ge cheng xiang guan ji yin de biao da 。RT-qPCRbiao ming ,MCMVqin ran hou yu mi SL1zhong BRxiang guan ji yin de biao da shang diao ;mei lian mian yi xi fu fen xi (enzyme-linked immunosorbent assay,ELISA)zheng ming ,MCMVqin ran hou yu mi SL1zhong BRde han liang zeng jia 。xie mian pen shi BRyou dao le yu mi dui MCMVde gan bing xing ,gai guo cheng ban sui zhao yi yang hua dan (nitric oxide,NO)de ji lei 。ran er ,dang wai shi NOqing chu ji shi ,BRdui yu mi gan bing xing de you dao xiao shi ,zhe biao ming BRyou dao de yu mi dui MCMVde gan bing xing yi lai yu NOji lei 。yi shang yan jiu jie guo biao ming ,BRtong guo yi lai NOde fang shi you dao yu mi dui MCMVde gan bing xing 。zhuai lu zu shu ju biao ming ,jie chong MCMVde yu mi zhong mo li suan (jasmonic acid,JA)ge cheng xiang guan yi ji shui yang suan (salicylic acid,SA)ge cheng xiang guan de ji yin biao da shang diao ,RT-qPCRzheng shi le zhe yi jie guo 。gao xiao ye xiang se pu fen xi (high performance liquid chromatography,HPLC)biao ming ,MCMVqin ran hou yu mi SL1zhong JAhe SAde han liang jun xian zhe zeng jia 。xie mian pen shi JAhuo SAjun neng zeng jiang yu mi dui MCMVde kang bing xing 。ran er ,tong shi pen shi JAhe SAshi ,yu mi zhi zhu dui MCMVkang xing de you dao cheng du ruo yu chan du pen shi JAhuo SA,zhe biao ming JA、SAzai jie dao yu mi dui MCMVkang bing xing de guo cheng zhong cun zai ming xian de jie kang zuo yong 。ci wai ,wo men hai fa xian BRneng gou zai yu mi gan ran MCMVguo cheng zhong de ji huo SA,zhu yao biao xian wei xie mian pen shi BRhou ,SAtu jing de biao ji ji yin ZmPR1ayi ji SAge cheng ji yin ZmPALsde biao da liang jun xian zhe sheng gao 。zeng ge MCMVqin ran yu mi hou BR、JAhe SAde xiang ying yi ji zhe ji chong ji su dui MCMVji lei de ying xiang ,wo men di chu le yi chong mo xing :MCMVde qin ran you dao le yu mi xie pian zhong BRde ji lei ,BRnong du de sheng gao yi fang mian cu jin le MCMVde ji lei ,ling yi fang mian ji huo le zhi wu ti nei de kang bing du ji su xin hao SA,SAji lei dao yi ding shui ping bian kai shi zhi heng MCMV,gai guo cheng xu yao NOcan yu 。ci wai ,MCMVqin ran ye you dao le yu mi xie pian zhong JAde ji lei ,JAneng you dao yu mi dui MCMVde kang xing ,dan JAyu SAcun zai xian zhe de jie kang zuo yong 。fu za de ji su ti ji wei chi le zhi zhu yu bing du de shi jun li di ,ben yan jiu cong ji su jiao du jie xi le ji su ji ji xin hao zhuai dao tu jing zai MCMVqin ran zhong de gong neng ,wei yan jiu MCMVde zhi bing ji zhi di gong le li lun yi ju 。
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论文详细介绍
论文作者分别是来自中国农业科学院的曹宁，发表于刊物中国农业科学院2019-07-05论文，是一篇关于转录组论文,玉米褪绿斑驳病毒论文,油菜素类固醇论文,茉莉酸论文,水杨酸论文,中国农业科学院2019-07-05论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自中国农业科学院2019-07-05论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：转录组论文; 玉米褪绿斑驳病毒论文; 油菜素类固醇论文; 茉莉酸论文; 水杨酸论文; 中国农业科学院2019-07-05论文;