本文主要研究内容
作者李继成（2019）在《外侧缰核在调节小鼠厌恶和奖赏行为中的作用及机制》一文中研究指出：奖赏和厌恶系统是调节动物机体“趋利避害”这一动机行为的重要保障,是生物生存、种族延续和生物进化的重要影响因素。奖赏刺激可产生愉悦、兴奋和欢快的感觉,进而导致趋向行为,并最终引导行为加强,诱发生物体有目的、有动机的行为反应,形成一种正性强化作用;相反地,厌恶刺激会导致生物产生恐惧、厌恶和反感等消极情绪,诱发回避行为,表现为一种负性强化效应。外侧缰核(LHb)作为脑内十分重要的负性行为调控中枢,也被称为“反奖赏中枢”,其与脑内重要的奖赏中枢——腹侧被盖区(VTA)之间不仅有着密切的纤维联系,而且二者均可对外界的各种奖赏或厌恶刺激产生反应,参与调节脑内奖赏和厌恶系统。但二者是如何汇聚奖赏和厌恶这两种相反的刺激信息,尤其是其中的单个神经元是否可汇聚并处理厌恶和奖赏这两种不同的传入信息,以及LHb中相关种类神经元对奖赏和厌恶行为的调节作用尚不清楚。在本研究中,结合多通道整体电生理和光遗传技术,我们记录了LHb和VTA中单个神经元分别对奖赏和厌恶刺激这两种不同的刺激信息的反应,分析了奖赏和厌恶信息在单个神经元中的汇聚情况,并进一步对LHb中对奖赏和厌恶刺激均有反应的神经元进行了鉴别,以明确其中谷氨酸神经元所占的比例。之后,结合光遗传技术和行为学检测,分别兴奋或抑制LHb中的谷氨酸神经元,或是VTA中来自LHb的谷氨酸神经纤维末梢,记录观察小鼠奖赏和厌恶相关行为的变化。结果显示,LHb神经元主要对奖赏刺激产生抑制反应,对厌恶刺激产生兴奋反应;而VTA神经元相反,其主要对奖赏刺激产生兴奋反应,对厌恶刺激产生抑制反应。在LHb和VTA中分别有75%和55%的单个神经元可汇聚奖赏和厌恶这两种信息的传入(汇聚神经元),但二者的反应趋势相反:LHb中77%的汇聚神经元为厌恶兴奋-奖赏抑制神经元,且主要是谷氨酸能神经元(88%);而VTA中51%的汇聚神经元是奖赏兴奋-厌恶抑制神经元。更为重要的是,兴奋LHb中的谷氨酸能神经元可明显抑制VTA中的这类汇聚神经元。行为学结果显示,不论是激活小鼠LHb谷氨酸神经元,抑或是激活LHb至VTA的谷氨酸能神经纤维投射均可引发厌恶行为;抑制LHb至VTA的谷氨酸神经纤维投射可产生奖赏行为,然而,抑制LHb谷氨酸神经元却未能诱发奖赏行为。我们的研究表明,厌恶中枢LHb中汇聚奖赏和厌恶这两种不同信息的单个谷氨酸神经元和奖赏中枢VTA中汇聚厌恶和奖赏信息的单个神经元间可能存在相互作用,并在机体奖赏和厌恶行为调节的过程中发挥重要作用。
Abstract
jiang shang he ya e ji tong shi diao jie dong wu ji ti “qu li bi hai ”zhe yi dong ji hang wei de chong yao bao zhang ,shi sheng wu sheng cun 、chong zu yan xu he sheng wu jin hua de chong yao ying xiang yin su 。jiang shang ci ji ke chan sheng yu yue 、xing fen he huan kuai de gan jiao ,jin er dao zhi qu xiang hang wei ,bing zui zhong yin dao hang wei jia jiang ,you fa sheng wu ti you mu de 、you dong ji de hang wei fan ying ,xing cheng yi chong zheng xing jiang hua zuo yong ;xiang fan de ,ya e ci ji hui dao zhi sheng wu chan sheng kong ju 、ya e he fan gan deng xiao ji qing xu ,you fa hui bi hang wei ,biao xian wei yi chong fu xing jiang hua xiao ying 。wai ce jiang he (LHb)zuo wei nao nei shi fen chong yao de fu xing hang wei diao kong zhong shu ,ye bei chen wei “fan jiang shang zhong shu ”,ji yu nao nei chong yao de jiang shang zhong shu ——fu ce bei gai ou (VTA)zhi jian bu jin you zhao mi qie de qian wei lian ji ,er ju er zhe jun ke dui wai jie de ge chong jiang shang huo ya e ci ji chan sheng fan ying ,can yu diao jie nao nei jiang shang he ya e ji tong 。dan er zhe shi ru he hui ju jiang shang he ya e zhe liang chong xiang fan de ci ji xin xi ,you ji shi ji zhong de chan ge shen jing yuan shi fou ke hui ju bing chu li ya e he jiang shang zhe liang chong bu tong de chuan ru xin xi ,yi ji LHbzhong xiang guan chong lei shen jing yuan dui jiang shang he ya e hang wei de diao jie zuo yong shang bu qing chu 。zai ben yan jiu zhong ,jie ge duo tong dao zheng ti dian sheng li he guang wei chuan ji shu ,wo men ji lu le LHbhe VTAzhong chan ge shen jing yuan fen bie dui jiang shang he ya e ci ji zhe liang chong bu tong de ci ji xin xi de fan ying ,fen xi le jiang shang he ya e xin xi zai chan ge shen jing yuan zhong de hui ju qing kuang ,bing jin yi bu dui LHbzhong dui jiang shang he ya e ci ji jun you fan ying de shen jing yuan jin hang le jian bie ,yi ming que ji zhong gu an suan shen jing yuan suo zhan de bi li 。zhi hou ,jie ge guang wei chuan ji shu he hang wei xue jian ce ,fen bie xing fen huo yi zhi LHbzhong de gu an suan shen jing yuan ,huo shi VTAzhong lai zi LHbde gu an suan shen jing qian wei mo sao ,ji lu guan cha xiao shu jiang shang he ya e xiang guan hang wei de bian hua 。jie guo xian shi ,LHbshen jing yuan zhu yao dui jiang shang ci ji chan sheng yi zhi fan ying ,dui ya e ci ji chan sheng xing fen fan ying ;er VTAshen jing yuan xiang fan ,ji zhu yao dui jiang shang ci ji chan sheng xing fen fan ying ,dui ya e ci ji chan sheng yi zhi fan ying 。zai LHbhe VTAzhong fen bie you 75%he 55%de chan ge shen jing yuan ke hui ju jiang shang he ya e zhe liang chong xin xi de chuan ru (hui ju shen jing yuan ),dan er zhe de fan ying qu shi xiang fan :LHbzhong 77%de hui ju shen jing yuan wei ya e xing fen -jiang shang yi zhi shen jing yuan ,ju zhu yao shi gu an suan neng shen jing yuan (88%);er VTAzhong 51%de hui ju shen jing yuan shi jiang shang xing fen -ya e yi zhi shen jing yuan 。geng wei chong yao de shi ,xing fen LHbzhong de gu an suan neng shen jing yuan ke ming xian yi zhi VTAzhong de zhe lei hui ju shen jing yuan 。hang wei xue jie guo xian shi ,bu lun shi ji huo xiao shu LHbgu an suan shen jing yuan ,yi huo shi ji huo LHbzhi VTAde gu an suan neng shen jing qian wei tou she jun ke yin fa ya e hang wei ;yi zhi LHbzhi VTAde gu an suan shen jing qian wei tou she ke chan sheng jiang shang hang wei ,ran er ,yi zhi LHbgu an suan shen jing yuan que wei neng you fa jiang shang hang wei 。wo men de yan jiu biao ming ,ya e zhong shu LHbzhong hui ju jiang shang he ya e zhe liang chong bu tong xin xi de chan ge gu an suan shen jing yuan he jiang shang zhong shu VTAzhong hui ju ya e he jiang shang xin xi de chan ge shen jing yuan jian ke neng cun zai xiang hu zuo yong ,bing zai ji ti jiang shang he ya e hang wei diao jie de guo cheng zhong fa hui chong yao zuo yong 。
论文参考文献
论文详细介绍
论文作者分别是来自吉林大学的李继成，发表于刊物吉林大学2019-06-25论文，是一篇关于外侧缰核论文,谷氨酸神经元论文,厌恶论文,奖赏论文,腹侧被盖区论文,光遗传论文,多通道电生理论文,吉林大学2019-06-25论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自吉林大学2019-06-25论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：外侧缰核论文; 谷氨酸神经元论文; 厌恶论文; 奖赏论文; 腹侧被盖区论文; 光遗传论文; 多通道电生理论文; 吉林大学2019-06-25论文;