本文主要研究内容
作者孟畅（2019）在《D~2型细胞质雄性不育小麦的鉴选及育性恢复的评价》一文中研究指出：植物雄性不育是高等植物一种非常普遍的生物学现象,在杂种优势的利用研究中具有重要意义。目前,细胞质雄性不育已成为杂种优势利用的重要手段。为了筛选理想的细胞质雄性不育小麦类型,获得恢复力较强的恢复系和强恢复组合,本研究以本课题组育成的D2型细胞质雄性不育系（Va706A、Ju706A、C6706A）为材料,通过对其进行花药碘化钾染色以及花药、小孢子的扫描电镜观察,明确不育系的败育特征及类型。以K型细胞质雄性不育系（K706A）为对照,将这4种小麦细胞质雄性不育系与本课题组筛选的128个恢复系进行杂交,通过对其F1代进行结实率和农艺性状的测定,比较D2型小麦雄性不育系与K型细胞质雄性不育系在育性恢复方面以及细胞质效应方面的异同点。获得以下试验结果:1.同核异质D2细胞质雄性不育小麦的生物学特征D2型、K型细胞质雄性不育系的花药与恢复系的花药在外形以及内部表型来看,都有明显的区别。恢复系LK783的花药外形挺直,基部开叉,外壁排布比较清晰有规律,小孢子的形状呈圆形,饱满,乌氏体排列紧密。花粉粒染色为均匀的黑色,形状规则、外形饱满,呈圆形。不育系的花药在外形方面形状较短、弯曲,花药的顶端是闭合的不散花粉,花药的外壁排布无序、杂乱无章褶皱,无条理,小孢子的形态不规则,不饱满,呈干瘪状,乌氏体排列稀疏。花粉粒染色不均匀不充分,C6706A属于典败,花粉粒形状不规则,干瘪,呈三角形。K706A、Ju706A、Va706A为染败或者典败。而恢复系LK783的花粉粒染色为均匀的黑色,形状规则、外形饱满,呈圆形。2.D2型细胞质雄性不育系的易恢性的测定筛选K型雄性不育系的F1平均结实率高于D2型细胞质雄性不育系。其中D2型细胞质雄性不育系:Ju706A的F1平均结实率最高,与父本223H2杂交后的结实率高达93.67%,其次是Va706A,其与R354-1杂交后的结实率为91.75%。平均结实率最低的是C6706A的F1,其与陕农33-1杂交结实率最高,为76.97%。从不育系恢复方面的稳定性来看,恢复能力最稳定的为C6706A,Ju706A的稳定性最差,居于中间位置是Va706A。由此可得出结论,相对于K型不育系而言,D2型细胞质雄性不育系其恢复稳定性均低于K型细胞质雄性不育系,易恢性表现为Ju706A>Va706A>C6706A,恢复稳定性表现为C6706A>Va706A>Ju706A。3.恢复系的筛选及评价通过恢复性异地鉴定,恢复系79107对四种不育系的平均结实率于三原、杨凌都是最高的,分别为75.14%、65.02%,该恢复系恢复能力比较强,也比较稳定,能较好的恢复不育系的育性。恢复系K460、A731/L783恢复能力也比较强,对于四个不育系的平均结实率分别为72.52%、75.51%。根据农艺性状（株高）与恢复能力综合筛选,两个恢复系79107、A731/L783可以恢复所有的不育系,有20个恢复系可以单独恢复K706A,有6个恢复系可以单独恢复Va706A,单独恢复Ju706A和C6706A的分别有4个。
Abstract
zhi wu xiong xing bu yo shi gao deng zhi wu yi chong fei chang pu bian de sheng wu xue xian xiang ,zai za chong you shi de li yong yan jiu zhong ju you chong yao yi yi 。mu qian ,xi bao zhi xiong xing bu yo yi cheng wei za chong you shi li yong de chong yao shou duan 。wei le shai shua li xiang de xi bao zhi xiong xing bu yo xiao mai lei xing ,huo de hui fu li jiao jiang de hui fu ji he jiang hui fu zu ge ,ben yan jiu yi ben ke ti zu yo cheng de D2xing xi bao zhi xiong xing bu yo ji （Va706A、Ju706A、C6706A）wei cai liao ,tong guo dui ji jin hang hua yao dian hua jia ran se yi ji hua yao 、xiao bao zi de sao miao dian jing guan cha ,ming que bu yo ji de bai yo te zheng ji lei xing 。yi Kxing xi bao zhi xiong xing bu yo ji （K706A）wei dui zhao ,jiang zhe 4chong xiao mai xi bao zhi xiong xing bu yo ji yu ben ke ti zu shai shua de 128ge hui fu ji jin hang za jiao ,tong guo dui ji F1dai jin hang jie shi lv he nong yi xing zhuang de ce ding ,bi jiao D2xing xiao mai xiong xing bu yo ji yu Kxing xi bao zhi xiong xing bu yo ji zai yo xing hui fu fang mian yi ji xi bao zhi xiao ying fang mian de yi tong dian 。huo de yi xia shi yan jie guo :1.tong he yi zhi D2xi bao zhi xiong xing bu yo xiao mai de sheng wu xue te zheng D2xing 、Kxing xi bao zhi xiong xing bu yo ji de hua yao yu hui fu ji de hua yao zai wai xing yi ji nei bu biao xing lai kan ,dou you ming xian de ou bie 。hui fu ji LK783de hua yao wai xing ting zhi ,ji bu kai cha ,wai bi pai bu bi jiao qing xi you gui lv ,xiao bao zi de xing zhuang cheng yuan xing ,bao man ,wu shi ti pai lie jin mi 。hua fen li ran se wei jun yun de hei se ,xing zhuang gui ze 、wai xing bao man ,cheng yuan xing 。bu yo ji de hua yao zai wai xing fang mian xing zhuang jiao duan 、wan qu ,hua yao de ding duan shi bi ge de bu san hua fen ,hua yao de wai bi pai bu mo xu 、za luan mo zhang zhe zhou ,mo tiao li ,xiao bao zi de xing tai bu gui ze ,bu bao man ,cheng gan bie zhuang ,wu shi ti pai lie xi shu 。hua fen li ran se bu jun yun bu chong fen ,C6706Ashu yu dian bai ,hua fen li xing zhuang bu gui ze ,gan bie ,cheng san jiao xing 。K706A、Ju706A、Va706Awei ran bai huo zhe dian bai 。er hui fu ji LK783de hua fen li ran se wei jun yun de hei se ,xing zhuang gui ze 、wai xing bao man ,cheng yuan xing 。2.D2xing xi bao zhi xiong xing bu yo ji de yi hui xing de ce ding shai shua Kxing xiong xing bu yo ji de F1ping jun jie shi lv gao yu D2xing xi bao zhi xiong xing bu yo ji 。ji zhong D2xing xi bao zhi xiong xing bu yo ji :Ju706Ade F1ping jun jie shi lv zui gao ,yu fu ben 223H2za jiao hou de jie shi lv gao da 93.67%,ji ci shi Va706A,ji yu R354-1za jiao hou de jie shi lv wei 91.75%。ping jun jie shi lv zui di de shi C6706Ade F1,ji yu shan nong 33-1za jiao jie shi lv zui gao ,wei 76.97%。cong bu yo ji hui fu fang mian de wen ding xing lai kan ,hui fu neng li zui wen ding de wei C6706A,Ju706Ade wen ding xing zui cha ,ju yu zhong jian wei zhi shi Va706A。you ci ke de chu jie lun ,xiang dui yu Kxing bu yo ji er yan ,D2xing xi bao zhi xiong xing bu yo ji ji hui fu wen ding xing jun di yu Kxing xi bao zhi xiong xing bu yo ji ,yi hui xing biao xian wei Ju706A>Va706A>C6706A,hui fu wen ding xing biao xian wei C6706A>Va706A>Ju706A。3.hui fu ji de shai shua ji ping jia tong guo hui fu xing yi de jian ding ,hui fu ji 79107dui si chong bu yo ji de ping jun jie shi lv yu san yuan 、yang ling dou shi zui gao de ,fen bie wei 75.14%、65.02%,gai hui fu ji hui fu neng li bi jiao jiang ,ye bi jiao wen ding ,neng jiao hao de hui fu bu yo ji de yo xing 。hui fu ji K460、A731/L783hui fu neng li ye bi jiao jiang ,dui yu si ge bu yo ji de ping jun jie shi lv fen bie wei 72.52%、75.51%。gen ju nong yi xing zhuang （zhu gao ）yu hui fu neng li zeng ge shai shua ,liang ge hui fu ji 79107、A731/L783ke yi hui fu suo you de bu yo ji ,you 20ge hui fu ji ke yi chan du hui fu K706A,you 6ge hui fu ji ke yi chan du hui fu Va706A,chan du hui fu Ju706Ahe C6706Ade fen bie you 4ge 。
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论文作者分别是来自西北农林科技大学的孟畅，发表于刊物西北农林科技大学2019-07-11论文，是一篇关于小麦论文,细胞质雄性不育论文,育性恢复论文,育性评价论文,西北农林科技大学2019-07-11论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自西北农林科技大学2019-07-11论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：小麦论文; 细胞质雄性不育论文; 育性恢复论文; 育性评价论文; 西北农林科技大学2019-07-11论文;